This invention discloses the use of an insecticidal
protein, specifically a method and its application for controlling lepidopteran pests. The method includes contacting the lepidopteran pests with the insecticidal
protein, or introducing a
nucleic acid molecule encoding the insecticidal
protein into a
plant, causing the lepidopteran pests to feed on the
plant. The insecticidal protein contains the
amino acid sequence shown in SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, or SEQ ID NO:20. In this invention, the Cry1Da1 proteins BD1-002, BD1-018, BD1-019, BD1-021, BD1-022, and BD1-022S exhibit good insecticidal activity against the soybean pest, the silver-striped armyworm.