Pest control method

A technology for pests and Spodoptera frugiperda, applied in the field of pest control

Active Publication Date: 2013-03-27
BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] It has been proved that plants transgenic for Cry1Ab gene can resist the attack of Lepid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example

[0078] The first example, the acquisition and synthesis of Cry1Ab gene

[0079] 1. Obtain the nucleotide sequence of Cry1Ab

[0080] The amino acid sequence (818 amino acids) of Cry1Ab-01 insecticidal protein, as shown in SEQ ID NO:1 in the sequence table; encoding Cry1Ab-01 corresponding to the amino acid sequence (818 amino acids) of the Cry1Ab-01 insecticidal protein The nucleotide sequence (2457 nucleotides) is shown in SEQ ID NO: 3 in the sequence listing.

[0081] The amino acid sequence (615 amino acids) of the Cry1Ab-02 insecticidal protein, as shown in SEQ ID NO: 2 in the sequence table; encodes Cry1Ab-02 corresponding to the amino acid sequence (615 amino acids) of the Cry1Ab-02 insecticidal protein The nucleotide sequence (1848 nucleotides) is shown in SEQ ID NO: 4 in the sequence listing.

[0082] 2. Synthesize the above Cry1Ab nucleotide sequence

[0083] The nucleotide sequence of Cry1Ab-01 (shown in SEQ ID NO: 3 in the sequence listing) and the nucleotide sequence of Cr...

Example

[0084] The second embodiment, construction of recombinant expression vector and transformation of Agrobacterium with recombinant expression vector

[0085] 1. Construction of a recombinant cloning vector containing Cry1Ab gene

[0086] Connect the synthesized Cry1Ab-01 nucleotide sequence to the cloning vector pGEM-T (Promega, Madison, USA, CAT: A3600), and proceed according to the instructions of Promega product pGEM-T vector to obtain the recombinant cloning vector DBN01-T , Its construction process is as figure 1 As shown (where Amp represents the ampicillin resistance gene; f1 represents the origin of replication of phage f1; LacZ is the LacZ start codon; SP6 is the SP6 RNA polymerase promoter; T7 is the T7 RNA polymerase promoter; Cry1Ab-01 It is the nucleotide sequence of Cry1Ab-01 (SEQ ID NO: 3); MCS is the multiple cloning site).

[0087] Then the recombinant cloning vector DBN01-T was transformed into E. coli T1 competent cells (Transgen, Beijing, China, CAT: CD501) by the ...

Example

[0096] The third embodiment, the acquisition and verification of corn plants transformed with Cry1Ab gene

[0097] 1. Obtain the corn plant transformed into Cry1Ab gene

[0098] According to the conventional Agrobacterium infection method, the aseptically cultivated immature embryos of maize variety Z31 (Z31) were co-cultured with the Agrobacterium described in 3 in the second embodiment to replace The T-DNA (including the promoter sequence of the maize Ubiquitin gene, the nucleotide sequence of Cry1Ab-01, the nucleotide sequence of Cry1Ab-02, the PMI gene and the Nos terminator sequence) in the recombinant expression vector DBN100124 and DBN100106 was transferred into the maize chromosome In the group, corn plants transformed with Cry1Ab-01 nucleotide sequence and corn plants transformed with Cry1Ab-02 nucleotide sequence were obtained; at the same time, wild-type corn plants were used as controls.

[0099] For Agrobacterium-mediated maize transformation, briefly, immature immature...

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Abstract

The invention relates to a method for controlling athetis lepigone pest, which comprises a step of enabling contact between athetis lepigone pest and Cry1A protein. According to the invention, Cry1A protein capable of killing athetis lepigone is generated in a plant body to control the athetis lepigone pest. Compared with the agricultural control method and chemical control method of the prior art, the method provided by the invention protects the full child-bearing period and full plant to control the invasion of the athetis lepigone pest, avoids pollution and residue, realizes a stable and thorough effect, and is simple, convenient and economical.

Description

technical field [0001] The invention relates to a method for controlling pests, in particular to a method for using Cry1A protein expressed in plants to control the damage of plants by Spodoptera spp. Background technique [0002] Athetis lepigone belongs to Lepidoptera Noctuidae and is an omnivorous pest. At present, it is found that it mainly harms corn crops. It is mainly distributed in the summer corn planting areas of Huanghuaihai in my country, and it is mainly distributed in Japan, North Korea, Russia, Europe and other places. The two-spot moth mainly harms the corn roots at the soil surface of the aerial roots of corn, and bites off the corn stems or superficial roots of the corn. A large area of ​​blank land appears, seriously endangering plots and even destroying species. [0003] Corn is the main food crop in China. On July 9, 2011, CCTV News Network reported for the first time the occurrence of Noctuid moth in China. From the autumn of 2011 to May 31, 2012, the ...

Claims

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Application Information

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IPC IPC(8): A01N47/44A01P7/04A01N63/50
CPCA01N63/02C07K14/325C12N15/8286A01N37/46A01H5/00Y02A40/146A01N63/50A01N63/23
Inventor 康越景庞洁张爱红程鹏杨旭牛丽红贾志伟安罗旭田康乐姜自芹
Owner BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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