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Method for controlling insect pest

A pest and protein technology, applied in chemical instruments and methods, botany equipment and methods, biochemical equipment and methods, etc.

Active Publication Date: 2013-07-10
BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] It has been proved that plants transgenic for Cry1B gene can resist Lepidoptera (Lepidoptera) pests such as corn borer and diamondback moth.

Method used

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  • Method for controlling insect pest
  • Method for controlling insect pest
  • Method for controlling insect pest

Examples

Experimental program
Comparison scheme
Effect test

no. 1 example

[0087] The first embodiment, the acquisition and synthesis of Cry1Ba gene

[0088] 1. Obtain the Cry1Ba nucleotide sequence

[0089] The amino acid sequence (1228 amino acids) of the Cry1Ba-01 insecticidal protein, as shown in SEQ ID NO: 1 in the sequence listing; Cry1Ba-01 encoding the amino acid sequence (1228 amino acids) corresponding to the Cry1Ba-01 insecticidal protein Nucleotide sequence (3687 nucleotides), as shown in SEQ ID NO:3 in the sequence listing; amino acid sequence (731 amino acids) of Cry1Ba-02 insecticidal protein, as shown in SEQ ID NO:2 in the sequence listing ; The Cry1Ba-02 nucleotide sequence (2196 nucleotides) encoding the amino acid sequence (731 amino acids) corresponding to the Cry1Ba-02 insecticidal protein, as shown in SEQ ID NO:4 in the sequence listing.

[0090] 2. Obtain the nucleotide sequences of Cry1Ab / Ac and Vip3A

[0091] The Cry1Ab / Ac nucleotide sequence (1830 nucleotides) of the amino acid sequence (609 amino acids) of coding Cry1Ab / A...

no. 2 example

[0094] The second embodiment, construction of recombinant expression vector and transformation of recombinant expression vector into Agrobacterium

[0095] 1. Construction of a recombinant cloning vector containing the Cry1B gene

[0096] The synthesized Cry1Ba-01 nucleotide sequence was connected to the cloning vector pGEM-T (Promega, Madison, USA, CAT: A3600), and the operation steps were carried out according to the instructions of the pGEM-T vector produced by Promega Company to obtain the recombinant cloning vector DBN01-T , its construction process is as follows figure 1 Shown (wherein, Amp represents the ampicillin resistance gene; f1 represents the replication origin of phage f1; LacZ is the LacZ initiation codon; SP6 is the SP6 RNA polymerase promoter; T7 is the T7 RNA polymerase promoter; Cry1Ba-01 is the Cry1Ba -01 nucleotide sequence (SEQ ID NO: 3); MCS is the multiple cloning site).

[0097] Then, the recombinant cloning vector DBN01-T was transformed into Esche...

no. 3 example

[0109] The third embodiment, the acquisition and verification of corn plants transferred to the Cry1B gene

[0110] 1. Obtaining corn plants transferred to the Cry1B gene

[0111] According to the commonly used Agrobacterium infection method, the immature embryos of the aseptically cultured corn variety Zong 31 (Z31) were co-cultured with the Agrobacterium described in 3 in the second embodiment, so that the T-DNA in recombinant expression vectors DBN100072, DBN100056 and DBN100083 (comprising the promoter sequence of corn Ubiquitin gene, Cry1Ba-01 nucleotide sequence, Cry1Ba-02 nucleotide sequence, Cry1Ab / Ac nucleotide sequence, Vip3A nucleotide sequence Acid sequence, PMI gene and Nos terminator sequence) were transferred into the maize genome, and the maize plant transferred to the Cry1Ba-01 nucleotide sequence and the maize plant transferred to the Cry1Ba-01-Cry1Ab / Ac nucleotide sequence were obtained and the corn plant transformed with the Cry1Ba-02-Vip3A nucleotide sequ...

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Abstract

The invention relates to a method for controlling pink rice borer pests, which is characterized by enabling the pink rice borer pests to be contacted with the Cry1B proteins. The method controls the pink rice borer pests through the Cry1B proteins which are produced in the plants and can kill pink rice borer pests. Compared with the agricultural control method, the chemical control method and the biological control method used in the prior art, the method performs whole period of duration and whole plant protection on the plants to prevent invasion of the pink rice borer pests; and the method has no pollution and no residue, and is stable and complete in effect, and simple, convenient and economical.

Description

technical field [0001] The invention relates to a method for controlling pests, in particular to a method for using Cry1B protein expressed in plants to control the plant damage caused by the large borer. Background technique [0002] Sesamia inferens belongs to Lepidoptera Noctuidae and is an omnivorous pest. In addition to maize, it also damages rice, sugarcane, sorghum and other gramineous crops. It is widely distributed in central and southeastern my country, especially in Shaanxi and Henan. Most of the rice areas to the south. The borer larvae bore into the stems of crops and cause damage to dead core seedlings or the whole plant. The boreholes are generally large, and a large amount of insect feces are discharged out of the stems. Occurs heavier than spring corn. [0003] Corn and sorghum are important food crops in China. The annual loss of food due to the large borer is huge, and even affects the living conditions of the local population. In order to prevent and co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G13/00C07K14/325C12N15/32A01N47/44A01N57/16A01P7/04A01N63/50
CPCC07K14/325C12N15/8286Y02A40/146A01N63/50
Inventor 丁德荣庞洁韩超
Owner BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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