Purpose of insecticidal protein

A protein and pest technology, applied in the field of insecticidal protein

Active Publication Date: 2015-09-09
BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Plants transgenic for Vip3A have been shown to be resistant to Lepidoptera pests such as cutworms, fall armyworm, Sorrel borer, and corn armyworm. Reports on Harm to Plants by the Strip Borer

Method used

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  • Purpose of insecticidal protein
  • Purpose of insecticidal protein
  • Purpose of insecticidal protein

Examples

Experimental program
Comparison scheme
Effect test

no. 1 example

[0082] The first embodiment, the acquisition and synthesis of genes

[0083] 1. Obtain the nucleotide sequence

[0084] The amino acid sequence (789 amino acids) of Vip3A-01 insecticidal protein, as shown in SEQ ID NO:1 in the sequence listing; The Vip3A-01 nucleotide sequence ( 2370 nucleotides), as shown in SEQ ID NO:2 in the sequence listing. Amino acid sequence (789 amino acids) of Vip3A-02 insecticidal protein, as shown in SEQ ID NO:3 in the sequence listing; Vip3A-02 nucleotide sequence ( 2370 nucleotides), as shown in SEQ ID NO:4 in the sequence listing.

[0085] The amino acid sequence (615 amino acids) of Cry1Ab insecticidal protein, as shown in SEQ ID NO:7 in the sequence listing; Cry1Ab nucleotide sequence (1848 nucleotides) of encoding corresponding to the amino acid sequence of said Cry1Ab insecticidal protein , as shown in SEQ ID NO:8 in the sequence listing.

[0086] The amino acid sequence (1436 amino acids) of Cry1Ab+Vip3A insecticidal protein, as shown in...

no. 2 example

[0089] The second embodiment, construction of recombinant expression vector and transformation of recombinant expression vector into Agrobacterium

[0090] 1. Construction of a recombinant cloning vector containing the Vip3A gene

[0091] The synthetic Vip3A-01 nucleotide sequence was connected to the cloning vector pGEM-T (Promega, Madison, USA, CAT: A3600), and the operation steps were carried out according to the instructions of the pGEM-T vector produced by Promega Company to obtain the recombinant cloning vector DBN01-T , its construction process is as follows figure 1 Shown (wherein, Amp represents the ampicillin resistance gene; f1 represents the replication origin of phage f1; LacZ is the LacZ initiation codon; SP6 is the SP6 RNA polymerase promoter; T7 is the T7 RNA polymerase promoter; Vip3A-01 is the Vip3A -01 nucleotide sequence (SEQ ID NO: 2); MCS is the multiple cloning site).

[0092]Then, the recombinant cloning vector DBN01-T was transformed into Escherichia...

no. 3 example

[0105] The third embodiment, the acquisition of transgenic plants

[0106] 1. Obtaining transgenic corn plants

[0107] According to the routinely used Agrobacterium infection method, the calli of the aseptically cultured corn variety Zong 31 (Z31) was co-cultured with the Agrobacterium described in 3 in the second embodiment to construct 2 in the second embodiment T-DNA in the recombinant expression vectors DBN100002 and DBN100003, DBN100740 and DBN100736 (comprising the promoter sequence of corn Ubiquitin gene, Vip3A-01 nucleotide sequence, Vip3A-02 nucleotide sequence, Cry1Ab+Vip3A nucleotide sequence, The Cry1Ab nucleotide sequence, the Hpt gene and the Nos terminator sequence) were transferred into the corn genome, and the corn plants transferred to the Vip3A-01 nucleotide sequence, the corn plant transferred to the Vip3A-02 nucleotide sequence, and the transferred Maize plants with Vip3A-01-Cry1Ab nucleotide sequence and maize plants transformed with Cry1Ab+Vip3A nucleo...

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Abstract

The invention relates to a purpose of an insecticidal protein. A method for controlling an injurious insect of proceras venosatus comprises at least contacting the injurious insect of proceras venosatus with a Vip3A protein. The Vip3A protein, which can kill the proceras venosatus, is generated in a plant body to control the injurious insect of proceras venosatus. Compared with an agricultural control method, a chemical control method and a physical control method in the prior art, the insecticidal protein can protect a whole plant in the whole growth period against the injurious insect of proceras venosatus. The insecticidal protein is free of pollution and residue, is stable and thorough in effect, and is simple, convenient and economic.

Description

technical field [0001] The invention relates to an application of an insecticidal protein, in particular to an application of a Vip3A protein expressed in plants to control damage to plants by the sorghum borer. Background technique [0002] The sorghum bar borer Chilo sacchariphagus belongs to the order Lepidoptera and belongs to the Mothidae family. Also known as sugarcane borer, commonly known as sorghum borer. It is mainly distributed in East Asia, South Asia and the Indian Ocean region such as China, India, Indonesia and Malaysia. The domestic distribution is very wide, and it occurs in most parts of Northeast, North China, East China and South China. Can harm corn, sorghum, millet, hemp, sugar cane and other crops. In dry grain areas in the north, it mainly damages crops such as corn, sorghum and millet, and often occurs in combination with corn borer, causing dead seedlings; it is also the main pest on sugarcane in the south. [0003] Corn is an important food cro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N47/44A01N57/16A01P7/04C12N15/82C12N15/32A01H1/02A01H5/00
CPCA01N47/44C12N15/82A01H1/123A01H1/127
Inventor 张爱红杨旭
Owner BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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