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Method for controlling injurious insects

A technology for pests and Spodoptera litura, applied in the direction of botany equipment and methods, biochemical equipment and methods, insecticides, etc.

Active Publication Date: 2014-04-16
BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Plants transgenic for Cry1A have been shown to be resistant to Lepidoptera pests such as corn borer, cotton bollworm, and fall armyworm (Fall Armyworm). Reports on Harm of Noctuid Moth to Plants

Method used

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  • Method for controlling injurious insects
  • Method for controlling injurious insects
  • Method for controlling injurious insects

Examples

Experimental program
Comparison scheme
Effect test

no. 1 example

[0092] The first embodiment, the acquisition and synthesis of Cry1A gene

[0093] 1. Obtain the Cry1A nucleotide sequence

[0094] The amino acid sequence (818 amino acids) of the Cry1Ab-01 insecticidal protein, as shown in SEQ ID NO: 1 in the sequence listing; Cry1Ab-01 encoding the amino acid sequence (818 amino acids) corresponding to the Cry1Ab-01 insecticidal protein Nucleotide sequence (2457 nucleotides), as shown in SEQ ID NO:4 in the sequence listing. The amino acid sequence (615 amino acids) of the Cry1Ab-02 insecticidal protein, as shown in SEQ ID NO: 2 in the sequence listing; Cry1Ab-02 encoding the amino acid sequence (615 amino acids) corresponding to the Cry1Ab-02 insecticidal protein Nucleotide sequence (1848 nucleotides), as shown in SEQ ID NO:5 in the sequence listing.

[0095] The amino acid sequence (609 amino acids) of the Cry1Ab / Ac insecticidal protein, as shown in SEQ ID NO:28 in the sequence listing; Cry1Ab / Ac encoding the amino acid sequence (609 amin...

no. 2 example

[0103] The second embodiment, construction of recombinant expression vector and transformation of recombinant expression vector into Agrobacterium

[0104] 1. Construction of a recombinant cloning vector containing the Cry1A gene

[0105] The synthesized Cry1Ab-01 nucleotide sequence was connected to the cloning vector pGEM-T (Promega, Madison, USA, CAT: A3600), and the operation steps were carried out according to the instructions of the pGEM-T vector produced by Promega Company to obtain the recombinant cloning vector DBN01-T , its construction process is as follows figure 1 Shown (wherein, Amp represents the ampicillin resistance gene; f1 represents the replication origin of phage f1; LacZ is the LacZ start codon; SP6 is the promoter of SP6 RNA polymerase; T7 is the promoter of T7 RNA polymerase; Cry1Ab-01 is the promoter of Cry1Ab -01 nucleotide sequence (SEQ ID NO: 4); MCS is a multiple cloning site).

[0106]Then, the recombinant cloning vector DBN01-T was transformed ...

no. 3 example

[0129] The third embodiment, the acquisition and verification of corn plants transferred to the Cry1A gene

[0130] 1. Obtaining corn plants transferred to the Cry1A gene

[0131] According to the commonly used Agrobacterium infection method, the immature embryos of the aseptically cultured maize variety Zong 31 (Z31) were co-cultured with the Agrobacterium described in 3 in the second example, so that the T-DNA in recombinant expression vectors DBN100124, DBN100053, DBN100056, DBN100003, DBN100029 and DBN100076 (including the promoter sequence of corn Ubiquitin gene, Cry1Ab-01 nucleotide sequence, Cry1Ab-02 nucleotide sequence, Cry1Ab / Ac nucleoside Acid sequence, Cry1A.105 nucleotide sequence, Vip3Aa nucleotide sequence, Cry2Ab nucleotide sequence, PMI gene and Nos terminator sequence) were transferred into the maize genome, and the transferred Cry1Ab-01 nucleotide sequence was obtained Corn plants transferred to Cry1Ab-02 nucleotide sequence, corn plants transferred to Cry1...

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Abstract

The invention relates to a method for controlling spodoptera litura. The method for controlling the spodoptera litura comprises the step that the spodoptera litura is made to make contact with CrylA protein. According to the method for controlling the spodoptera litura, the spodoptera litura is controlled through the CrylA protein which is generated by bodies of plants and is capable of killing the spodoptera litura. Compared with an agricultural control method, a chemical control method and a physical control method adopted in the prior art, the method for controlling the spodoptera litura carries out protection on all growth periods and all plants to control the infringement of the spodoptera litura, and is free of pollution and residues, stable and thorough in effect, simple, convenient to implement and economical.

Description

technical field [0001] The invention relates to a method for controlling pests, in particular to a method for controlling Spodoptera litura from damaging plants by using Cry1A protein expressed in plants. Background technique [0002] Spodoptera litura belongs to Lepidoptera Noctuidae. It is an omnivorous and gluttonous pest. It damages a wide range of hosts. In addition to corn and soybeans, it can also damage melons, eggplants, beans, green onions, leeks, spinach and There are nearly 100 families and more than 300 kinds of plants including cruciferous vegetables, grains, and cash crops. Spodoptera litura is distributed worldwide and occurs in all parts of the country, mainly in the Yangtze River Basin and the Yellow River Basin. Spodoptera litura mainly damages the whole plant with larvae, and gathers and eats on the back of leaves when it is young; after the third instar, it disperses and damages leaves, tender stems, and old larvae can eat fruits. [0003] Corn and soyb...

Claims

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Application Information

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IPC IPC(8): A01G13/00A01H5/00A01N47/44A01P7/04
CPCA01N37/46C12N15/8286Y02A40/146
Inventor 庞洁丁德荣田聪杨旭张云珠
Owner BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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