Method for controlling pests

A pest and protein technology, applied in the fields of botany equipment and methods, biochemical equipment and methods, angiosperms/flowering plants, etc.

Active Publication Date: 2013-03-20
BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] It has been proved that the plants transgenic for Cry1F can resist the attack of Lepidoptera pests such as the small cutworm. However, there is no report on the control of the plant damage caused by the large borer by producing transgenic plants expressing the Cry1F protein.

Method used

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  • Method for controlling pests
  • Method for controlling pests
  • Method for controlling pests

Examples

Experimental program
Comparison scheme
Effect test

no. 1 example

[0084] The first embodiment, the acquisition and synthesis of Cry1Fa gene

[0085] 1. Obtain the Cry1Fa nucleotide sequence

[0086] The amino acid sequence (605 amino acids) of the Cry1Fa-01 insecticidal protein, as shown in SEQ ID NO: 1 in the sequence listing; Cry1Fa-01 encoding the amino acid sequence (605 amino acids) corresponding to the Cry1Fa-01 insecticidal protein Nucleotide sequence (1818 nucleotides), as shown in SEQ ID NO:3 in the sequence listing; amino acid sequence (1148 amino acids) of Cry1Fa-02 insecticidal protein, as shown in SEQ ID NO:2 in the sequence listing ; Cry1Fa-02 nucleotide sequence (3447 nucleotides) encoding the amino acid sequence (1148 amino acids) corresponding to the Cry1Fa-02 insecticidal protein, as shown in SEQ ID NO:4 in the sequence listing.

[0087] 2. Obtain the nucleotide sequences of Cry1Ab and Vip3A

[0088] The Cry1Ab nucleotide sequence (1848 nucleotides) encoding the amino acid sequence (615 amino acids) of the Cry1Ab insectic...

no. 2 example

[0091] The second embodiment, construction of recombinant expression vector and transformation of recombinant expression vector into Agrobacterium

[0092] 1. Construction of a recombinant cloning vector containing the Cry1F gene

[0093] The synthesized Cry1Fa-01 nucleotide sequence was connected to the cloning vector pGEM-T (Promega, Madison, USA, CAT: A3600), and the operation steps were carried out according to the instructions of the pGEM-T vector produced by Promega Company to obtain the recombinant cloning vector DBN01-T , its construction process is as follows figure 1 Shown (wherein, Amp represents the ampicillin resistance gene; f1 represents the replication origin of phage f1; LacZ is the LacZ start codon; SP6 is the SP6 RNA polymerase promoter; T7 is the T7 RNA polymerase promoter; Cry1Fa-01 is the Cry1Fa-01 nucleotide sequence (SEQ ID NO: 3); MCS is the multiple cloning site).

[0094] Then, the recombinant cloning vector DBN01-T was transformed into Escherichia...

no. 3 example

[0106] The third embodiment, the acquisition and verification of corn plants transferred to the Cry1F gene

[0107] 1. Obtaining corn plants transferred to the Cry1F gene

[0108] According to the commonly used Agrobacterium infection method, the immature embryos of the aseptically cultured maize variety Zong 31 (Z31) were co-cultured with the Agrobacterium described in 3 in the second example, so that the T-DNA in recombinant expression vectors DBN100014, DBN100012 and DBN100276 (including the promoter sequence of corn Ubiquitin gene, Cry1Fa-01 nucleotide sequence, Cry1Fa-02 nucleotide sequence, Cry1Ab nucleotide sequence, Vip3A nucleotide sequence , PMI gene and Nos terminator sequence) were transferred into the maize genome, and the maize plants transferred to the Cry1Fa-01 nucleotide sequence, the maize plants transferred to the Cry1Fa-01-Cry1Ab nucleotide sequence and the transferred Cry1Fa - the maize plant of 02-Vip3A nucleotide sequence; meanwhile, the wild type maize...

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Abstract

The invention relates to a method for controlling Sesamia inferens, comprising a step of contacting the Sesamia inferens with a Cry1F protein. By the method for controlling pests, the Sesamia inferens is controlled by the Cry1F protein which can be generated in plant bodies and can kill the Sesamia inferens. Compared with the agricultural control methods, the chemical control methods and the biological control methods used in the prior art, the method disclosed by the invention protects the whole plant during a whole growth period so as to control the disoperation of the Sesamia inferens, and is pollution-free, residue-free, stable and thorough in effect, simple, convenient and economical.

Description

technical field [0001] The invention relates to a method for controlling pests, in particular to a method for using Cry1F protein expressed in plants to control the plant damage caused by the large borer. Background technique [0002] Sesamia inferens belongs to Lepidoptera Noctuidae. It is an omnivorous pest. In addition to corn, it also damages rice, sugarcane, sorghum and other gramineous crops. It is widely distributed in central and southeastern my country, especially in Shaanxi and Henan. Most of the rice areas to the south. The borer larvae bore into the stems of crops and cause damage to dead core seedlings or the whole plant. The boreholes are generally large, and a large amount of insect feces are discharged out of the stems. Occurs heavier than spring corn. [0003] Corn and sorghum are important food crops in China. The annual loss of food due to the large borer is huge, and even affects the living conditions of the local population. In order to prevent and con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G13/00C12N15/84A01H5/00A01N63/50
CPCA01N63/02C12N15/8286Y02A40/146A01N63/50A01N25/00
Inventor 韩超庞洁康越景刘海利张云珠张成伟许春苹魏玫黄金存田康乐王乾钦
Owner BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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