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Method for controlling pests

A pest, protein technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, pesticides, etc.

Active Publication Date: 2014-04-16
BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] It has been proved that the plants transgenic for Cry1A can resist the attack of Lepidoptera pests such as corn borer, cotton bollworm, and fall armyworm. However, there are few reports on the control of plant damage caused by Cry1A protein by producing transgenic plants expressing Cry1A protein

Method used

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  • Method for controlling pests
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Examples

Experimental program
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Effect test

no. 1 example

[0089] The first embodiment, the acquisition and synthesis of Cry1A gene

[0090] 1. Obtain the Cry1A nucleotide sequence

[0091] The amino acid sequence (818 amino acids) of the Cry1Ab-01 insecticidal protein, as shown in SEQ ID NO: 1 in the sequence listing; Cry1Ab-01 encoding the amino acid sequence (818 amino acids) corresponding to the Cry1Ab-01 insecticidal protein Nucleotide sequence (2457 nucleotides), as shown in SEQ ID NO:4 in the sequence listing. The amino acid sequence (615 amino acids) of the Cry1Ab-02 insecticidal protein, as shown in SEQ ID NO: 2 in the sequence listing; Cry1Ab-02 encoding the amino acid sequence (615 amino acids) corresponding to the Cry1Ab-02 insecticidal protein Nucleotide sequence (1848 nucleotides), as shown in SEQ ID NO:5 in the sequence listing.

[0092] The amino acid sequence (1177 amino acids) of the Cry1A.105 insecticidal protein, as shown in SEQ ID NO: 3 in the sequence listing; Cry1A.105 encoding the amino acid sequence (1177 am...

no. 2 example

[0099] The second embodiment, construction of recombinant expression vector and transformation of recombinant expression vector into Agrobacterium

[0100] 1. Construction of a recombinant cloning vector containing the Cry1A gene

[0101] The synthesized Cry1Ab-01 nucleotide sequence was connected to the cloning vector pGEM-T (Promega, Madison, USA, CAT: A3600), and the operation steps were carried out according to the instructions of the pGEM-T vector produced by Promega Company to obtain the recombinant cloning vector DBN01-T , its construction process is as follows figure 1 Shown (wherein, Amp represents the ampicillin resistance gene; f1 represents the replication origin of phage f1; LacZ is the LacZ start codon; SP6 is the promoter of SP6 RNA polymerase; T7 is the promoter of T7 RNA polymerase; Cry1Ab-01 is the promoter of Cry1Ab -01 nucleotide sequence (SEQ ID NO: 4); MCS is a multiple cloning site).

[0102] Then, the recombinant cloning vector DBN01-T was transformed...

no. 3 example

[0117] The third embodiment, the acquisition and verification of corn plants transferred to the Cry1A gene

[0118] 1. Obtaining corn plants transferred to the Cry1A gene

[0119] According to the commonly used Agrobacterium infection method, the immature embryos of the aseptically cultured maize variety Zong 31 (Z31) were co-cultured with the Agrobacterium described in 3 in the second example, so that the T-DNA in recombinant expression vectors DBN100124, DBN100053, DBN100003, DBN100029 and DBN100076 (including the promoter sequence of corn Ubiquitin gene, Cry1Ab-01 nucleotide sequence, Cry1Ab-02 nucleotide sequence, Cry1A.105 nucleotide sequence , Vip3Aa nucleotide sequence, Cry2Ab nucleotide sequence, PMI gene and Nos terminator sequence) were transferred into the maize genome, and the maize plants transferred to the Cry1Ab-01 nucleotide sequence and the Cry1Ab-02 nuclear The corn plant of nucleotide sequence, the corn plant of Cry1Ab-01-Vip3Aa nucleotide sequence, the cor...

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Abstract

The invention relates to a method for controlling pests. The method includes the step that the pests, namely sesamia inferens, are in contact with CrylA protein. The sesamia inferens are controlled in the mode that the CrylA protein capable of killing the sesamia inferens is provided in plants. Compared with an agricultural prevention method, a chemical prevention method and a biological prevention method in the prior art, the method can protect the whole plants during the whole growth period so as to prevent attack of the sesamia inferens, and is free of pollution, free of residual pesticide, stable and complete in effect, simple, convenient to carry out and economical.

Description

technical field [0001] The invention relates to a method for controlling pests, in particular to a method for using Cry1A protein expressed in plants to control plants caused by the large borer. Background technique [0002] Sesamia inferens belongs to Lepidoptera Noctuidae and is an omnivorous pest. In addition to maize, it also harms rice, sugarcane, wheat, sorghum and other gramineous crops. It is widely distributed in central and southeastern my country and Southeast Asian rice, Sugar cane producing area. The larvae of the large borer bore into the stems of the crops and cause damage, which can cause the death of corn seedlings or the whole plant, and the phenomenon of dead hearts or white ears of rice. Especially in recent years due to global warming, the occurrence of the large borer has been increasing year by year and moving northward. [0003] Corn and rice are important food crops in China. The annual loss of food due to the large borer is huge, and even affects t...

Claims

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Application Information

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IPC IPC(8): A01G13/00A01H5/00A01N47/44A01P7/04
CPCA01N37/46C07K14/325C12N15/8286Y02A40/146
Inventor 韩超于彩虹丁德荣张欣馨岳健婷
Owner BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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