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Pest control method

A technology for pests and Spodoptera litura, applied in the direction of botany equipment and methods, biochemical equipment and methods, insecticides, etc.

Inactive Publication Date: 2014-04-16
BEIJING DABEINONG TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] It has been proved that the plants transgenic of Vip3A gene can resist the attack of Lepidoptera pests such as cutworm, fall armyworm, large borer, corn armyworm, etc. Reports on Controlling Spodoptera litura Harms to Plants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

no. 1 example

[0088] The first embodiment, the acquisition and synthesis of Vip3A gene

[0089] 1. Obtain the nucleotide sequence of Vip3A

[0090] Amino acid sequence (789 amino acids) of Vip3Aa-01 insecticidal protein, as shown in SEQ ID NO: 1 in the sequence listing; Vip3Aa-01 encoding the amino acid sequence (789 amino acids) corresponding to said Vip3Aa-01 insecticidal protein Nucleotide sequence (2370 nucleotides), as shown in SEQ ID NO:3 in the sequence listing. Amino acid sequence (789 amino acids) of the Vip3Aa-02 insecticidal protein, as shown in SEQ ID NO: 2 in the sequence listing; Vip3Aa-02 encoding the amino acid sequence (789 amino acids) corresponding to the Vip3Aa-02 insecticidal protein Nucleotide sequence (2370 nucleotides), as shown in SEQ ID NO:4 in the sequence listing.

[0091] 2. Obtain the nucleotide sequences of Cry1A and Cry1F

[0092] The Cry1Ab nucleotide sequence (2457 nucleotides) encoding the amino acid sequence (818 amino acids) of the Cry1Ab insecticidal...

no. 2 example

[0095] The second embodiment, construction of recombinant expression vector and transformation of recombinant expression vector into Agrobacterium

[0096] 1. Construction of a recombinant cloning vector containing the Vip3A gene

[0097] The synthetic Vip3Aa-01 nucleotide sequence was ligated into the cloning vector pGEM-T (Promega, Madison, USA, CAT: A3600), and the operation steps were carried out according to the instructions of the pGEM-T vector produced by Promega Company to obtain the recombinant cloning vector DBN01-T , its construction process is as follows figure 1 Shown (wherein, Amp represents the ampicillin resistance gene; f1 represents the replication origin of phage f1; LacZ is the LacZ start codon; SP6 is the promoter of SP6 RNA polymerase; T7 is the promoter of T7 RNA polymerase; Vip3Aa-01 is the promoter of Vip3Aa -01 nucleotide sequence (SEQ ID NO: 3); MCS is a multiple cloning site).

[0098] Then, the recombinant cloning vector DBN01-T was transformed i...

no. 3 example

[0113] The third embodiment, the acquisition and verification of corn plants transferred to Vip3A gene

[0114] 1. Obtaining corn plants transferred to the Vip3A gene

[0115] According to the commonly used Agrobacterium infection method, the immature embryos of the aseptically cultured maize variety Zong 31 (Z31) were co-cultured with the Agrobacterium described in 3 in the second example, so that the T-DNA in recombinant expression vectors DBN100066, DBN100003 and DBN100276 (including the promoter sequence of corn Ubiquitin gene, Vip3Aa-01 nucleotide sequence, Vip3Aa-02 nucleotide sequence, Cry1Ab nucleotide sequence, Cry1Fa nucleotide sequence , PMI gene and Nos terminator sequence) were transferred into the maize genome, and the maize plants transferred to the Vip3Aa-01 nucleotide sequence, the maize plants transferred to the Vip3Aa-01-Cry1Ab nucleotide sequence and the Vip3Aa -02-Cry1Fa nucleotide sequence of maize plants; meanwhile wild-type maize plants are used as con...

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Abstract

The invention relates to a method for controlling prodenia litura pests. The method comprises the step that the prodenia litura pests are contacted with Vip3A protein. According to the invention, the prodenia litura pests are controlled by Vip3A protein which is generated in plants and can kill prodenia litura; and compared with an agricultural control method, a chemical control method and a physical control method used in the prior art, the pest control method can be used for protecting whole plants at the whole growth periods so as to prevent and control attack of the prodenia litura pests, and is further free of pollution and residue, stable and thorough in effect, simple, convenient and economical.

Description

technical field [0001] The invention relates to a method for controlling pests, in particular to a method for using Vip3A protein expressed in plants to control Spodoptera litura from damaging plants. Background technique [0002] Spodoptera litura belongs to Lepidoptera Noctuidae. It is an omnivorous and gluttonous pest. It damages a wide range of hosts. In addition to corn and soybeans, it can also damage melons, eggplants, beans, green onions, leeks, spinach and There are nearly 100 families and more than 300 kinds of plants including cruciferous vegetables, grains, and cash crops. Spodoptera litura is distributed worldwide and occurs in all parts of the country, mainly in the Yangtze River Basin and the Yellow River Basin. Spodoptera litura mainly damages the whole plant with larvae, and gathers and eats on the back of leaves when it is young; after the third instar, it disperses and damages leaves, tender stems, and old larvae can eat fruits. [0003] Corn and soybeans...

Claims

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Application Information

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IPC IPC(8): A01N47/44A01N57/16A01P7/04C12N15/84A01H5/00
CPCA01N37/46C07K14/325C12N15/8286Y02A40/146
Inventor 康越景王登元焦国伟田聪张云珠
Owner BEIJING DABEINONG TECH GRP CO LTD
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