Pest control method

A kind of pest, peach borer technology, applied in the field of pest control

Active Publication Date: 2013-03-20
BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] It has been proved that the plants transgenic for Cry1A can resist the attack of Lepidoptera pests such as corn borer, cotton bollworm, and rice stem borer. Hazard Reporting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

no. 1 example

[0087] The first embodiment, the acquisition and synthesis of Cry1A gene

[0088] 1. Obtain the Cry1A nucleotide sequence

[0089] The amino acid sequence (818 amino acids) of the Cry1Ab-01 insecticidal protein, as shown in SEQ ID NO: 1 in the sequence listing; Cry1Ab-01 encoding the amino acid sequence (818 amino acids) corresponding to the Cry1Ab-01 insecticidal protein Nucleotide sequence (2457 nucleotides), as shown in SEQ ID NO:4 in the sequence listing. The amino acid sequence (615 amino acids) of the Cry1Ab-02 insecticidal protein, as shown in SEQ ID NO: 2 in the sequence listing; Cry1Ab-02 encoding the amino acid sequence (615 amino acids) corresponding to the Cry1Ab-02 insecticidal protein Nucleotide sequence (1848 nucleotides), as shown in SEQ ID NO:5 in the sequence listing.

[0090] The amino acid sequence (699 amino acids) of the Cry1Ah insecticidal protein, as shown in SEQ ID NO:3 in the sequence listing; the Cry1Ah-01 nucleotide sequence encoding the amino aci...

no. 2 example

[0095] The second embodiment, construction of recombinant expression vector and transformation of recombinant expression vector into Agrobacterium

[0096] 1. Construction of a recombinant cloning vector containing the Cry1A gene

[0097] The synthesized Cry1Ab-01 nucleotide sequence was connected to the cloning vector pGEM-T (Promega, Madison, USA, CAT: A3600), and the operation steps were carried out according to the instructions of the pGEM-T vector produced by Promega Company to obtain the recombinant cloning vector DBN01-T , its construction process is as follows figure 1 Shown (wherein, Amp represents the ampicillin resistance gene; f1 represents the replication origin of phage f1; LacZ is the LacZ initiation codon; SP6 is the SP6 RNA polymerase promoter; T7 is the T7 RNA polymerase promoter; Cry1Ab-01 is the Cry1Ab-01 nucleotide sequence (SEQ ID NO: 4); MCS is the multiple cloning site).

[0098] Then, the recombinant cloning vector DBN01-T was transformed into Escheric...

no. 3 example

[0113] The third embodiment, the acquisition and verification of corn plants transferred to the Cry1A gene

[0114] 1. Obtaining corn plants transferred to the Cry1A gene

[0115] According to the commonly used Agrobacterium infection method, the immature embryos of the aseptically cultured maize variety Zong 31 (Z31) were co-cultured with the Agrobacterium described in 3 in the second example, so that the T-DNA in recombinant expression vectors DBN100124, DBN100075, DBN100071 and DBN100147 (including the promoter sequence of corn Ubiquitin gene, Cry1Ab-01 nucleotide sequence, Cry1Ab-02 nucleotide sequence, Cry1Ah-01 nucleotide sequence, Cry1Ah -02 nucleotide sequence, Cry1Fa nucleotide sequence, Cry1Ie nucleotide sequence, PMI gene and Nos terminator sequence) were transferred into the corn genome, and the corn plants transferred to the Cry1Ab-01 nucleotide sequence were obtained, Corn plants transferred to the Cry1Ab-02-Cry1Fa nucleotide sequence, corn plants transferred to...

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Abstract

The invention relates to a method for controlling conogethes punctiferalis pests. The method comprises the following step of: enabling the conogethes punctiferalis to be in contact with Cry1A protein. The method can be used for controlling the conogethes punctiferalis pests by using the Cry1A protein which is produced in plant bodies and is capable of killing the conogethes punctiferalis. Compared with the agricultural control methods, the chemical control methods and the biological control methods in the prior art, the method for controlling the conogethes punctiferalis pests has the capability of controlling the infringe of the conogethes punctiferalis by carrying out protection on plants in the whole growth period and the whole plants and is free of pollution and residual, stable and thorough in effect, simple, convenient and economical.

Description

technical field [0001] The invention relates to a method for controlling pests, in particular to a method for using Cry1A protein expressed in plants to control the damage of plants by the peach borer. Background technique [0002] Peach borer (Conogethes punctiferalis) belongs to Lepidoptera Mothidae and is an omnivorous pest. It not only harms corn, sorghum and other crops, but also harms peach, persimmon, chestnut and other fruit trees. It is widely distributed in my country, starting from Heilongjiang and Inner Mongolia in the north. , south to Taiwan, Hainan, Guangdong, Guangxi, and the southern edge of Yunnan, east to the former Soviet Union, North Korea, west from Shanxi, Shaanxi to Ningxia, Gansu, and then to Sichuan, Yunnan, and Tibet. When maize is harmed, it mainly eats the ears and stems, and the plant damage rate reaches 30%-80%. When harming sorghum, the newly hatched larvae eat into the young sorghum grains, and seal the mouth with feces or food residues. Ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N47/44A01P7/04A01N63/50
CPCC07K14/325C12N15/8286A01N63/02A01N37/46C12N15/82Y02A40/146A01N63/50A01N63/23
Inventor 李胜兵康越景程鹏牛瑞琪刘敬宋肖倩刘丽张进峰田康乐刘彦晓
Owner BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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