Hormone-free tissue culture method for dendrobium candidum

A Dendrobium candidum, tissue culture technology, applied in the field of agriculture, to achieve the effect of reducing production costs, low cost, resource waste and time consumption

Inactive Publication Date: 2012-08-01
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the current problem of using various hormones in the tissue culture process of Dendrobium officinale to provide a more environmentally friendly, green, low-cost, and convenient hormo

Method used

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  • Hormone-free tissue culture method for dendrobium candidum
  • Hormone-free tissue culture method for dendrobium candidum
  • Hormone-free tissue culture method for dendrobium candidum

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Dendrobium officinale hormone-free tissue culture method:

[0021] a) The mature Dendrobium candidum capsules or Dendrobium candidum seeds collected from the field are sterilized, planted in hormone-free MS medium, and cultivated for 25 days. Seed embryos expand and germinate to form green round bulbs; culture conditions: culture temperature 23~ 27℃, illuminance 1500~2000lx, light time 12h / d;

[0022] b) Transfer the bulbs into hormone-free basic MS basic medium + mashed potato 20g / l medium, and cultivate them for 25 days. The bulbs overlap and pile up in the hormone-free medium, and leaf primordia and differentiated seedlings appear; Culture conditions: culture temperature 23~27℃, light intensity 1500~2000lx, light time 12h / d;

[0023] c) transfer the round corm differentiated seedlings into the hormone-free MS basic medium+mashed banana 15g / l+mashed potato 15g / l medium, after 20 to 30 days of induction, leaf primordia and seedlings reaching 1 to 2 cm in height appear...

Embodiment 2

[0031] Dendrobium officinale hormone-free tissue culture method:

[0032] a) The mature Dendrobium candidum capsules or Dendrobium candidum seeds collected from the field are sterilized, planted in hormone-free MS medium, and cultivated for 30 days. Seed embryos expand and germinate to form green round bulbs; culture conditions: culture temperature 23~ 27℃, illuminance 1500~2000lx, light time 12h / d;

[0033] b) Transfer the bulbs into the hormone-free basic MS basic medium + mashed potato 15g / l medium, and cultivate them for 30 days. The bulbs overlap and pile up in the hormone-free medium, and leaf primordia and differentiated seedlings appear; Culture conditions: culture temperature 23~27℃, light intensity 1500~2000lx, light time 12h / d;

[0034] c) transfer the round corm differentiated seedlings into hormone-free MS basic medium+mashed banana 20g / l+mashed potato 20g / l medium, after 20 to 30 days of induction, leaf primordium and a height of 1 to 2cm seedlings appear, and t...

Embodiment 3

[0042] Hormone culture: The medium used for seed germination into spherical bulbs is MS basic medium + sucrose 30g / l + agar 6g / l + 6-BA (benzylaminopurine) 0.5mg / l + NAA (α-naphthaleneacetic acid) 0.2mg / l , The medium used for proliferation and differentiation from round bulbs is MS basic medium + sucrose 30g / l + agar 6g / l + 6-BA (benzylaminopurine) 0.5mg / l + NAA (α-naphthaleneacetic acid) 0.2mg / l, The culture medium used from the differentiated seedling to the stem section is MS basic medium+sucrose 30g / l+agar 6g / l+6-BA (benzylaminopurine) 0.5mg / l+NAA (α-naphthaleneacetic acid) 0.5mg / l), The medium used for rooting and strong seedlings is 1 / 2 MS basic medium + sucrose 30g / l + agar 6g / l + 6-BA (benzylaminopurine) 2mg / l + NAA (α-naphthaleneacetic acid) 0.5mg / l, and the cultivation method is the same as In Example 1, the culture time is 30-35 days, 30-35 days, 60-65 days, and 35-40 days, respectively.

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Abstract

The invention discloses a hormone-free tissue culture method for dendrobium candidum, which comprises four tissue culture stages including germinating of seeds of the dendrobium candidum into spherical stems, multiplication and differentiation, and rootage and sound seedlings at stem sections; culture media used at the four stages include a hormone-free MS (Murashige and Skoog) culture medium, a hormone-free MS basal culture medium plus 15-20 g/l mashed potato culture medium, a hormone-free MS basal culture medium plus 15-20 g/l mashed banana plus 15-20 g/l mashed potato culture medium and a hormone-free 1/2 MS basal culture medium plus 15-20 g/l mashed banana plus 15-20 g/l mashed potato culture medium. The polysaccharide content and total alkaloid content in the culture of the dendrobium candidum obtained by hormone-free culture are increased compared with hormone-containing culture; the growth state of the dendrobium candidum is normal; the genetic diversity of the plant variety is protected; with the adoption of the method, the tissue culture of the dendrobium candidum is more convenient, green and environmentally friendly, and the resources are saved.

Description

technical field [0001] The invention belongs to the field of agricultural technology, and in particular relates to a hormone-free tissue culture method for Dendrobium officinale. Background technique [0002] Dendrobium officinale (Dendrobium officinale) is a perennial herb belonging to epiphytic orchids. It has been listed as the top grade in Materia Medica, and it has been confirmed that it has the functions of nourishing yin and clearing heat, promoting body fluid and benefiting stomach, relieving cough and moistening lung, moistening throat and improving eyesight, improving human immune function, preventing tumor and cardiovascular disease, improving sleep, preventing cataract, and prolonging life. and other effects. However, due to the special growth environment, limited distribution, and long-term mining, the natural resources are becoming increasingly depleted. The country has been listed as a key protected wild medicinal material and encourages artificial large-scal...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 杨岚向增旭
Owner NANJING AGRICULTURAL UNIVERSITY
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