Flavonoid 3',5'-hydroxylase gene functional marker for screening high-dihydroxyl catechin-content tea plant, as well as application and application method thereof

A high dihydroxy catechin and gene function technology, applied in biochemical equipment and methods, microbial measurement/testing, DNA/RNA fragments, etc., can solve the problems of long time-consuming and low efficiency, and speed up the pace of breeding Effect

Active Publication Date: 2017-05-31
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method requires a certain amount of tea tree leaves, and the identified plants need to grow up to 3-4 years before they can be identified, which takes too long and is inefficient

Method used

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  • Flavonoid 3',5'-hydroxylase gene functional marker for screening high-dihydroxyl catechin-content tea plant, as well as application and application method thereof
  • Flavonoid 3',5'-hydroxylase gene functional marker for screening high-dihydroxyl catechin-content tea plant, as well as application and application method thereof
  • Flavonoid 3',5'-hydroxylase gene functional marker for screening high-dihydroxyl catechin-content tea plant, as well as application and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] F3'5'H Discovery of Allelic Difference Sequence, Primer Pair and Development of Functional Marker dCAPS-F3′5′H

[0022] 1. Test materials

[0023] Choose Longjing 43 and Fenghuang tea tree as research materials.

[0024] 2. Determination of catechin content

[0025] Pick one bud and two leaves of the new shoots of tea trees in spring, dry them with hot air at 120°C for 5 minutes to fix the samples in time, and dry them at 75°C until they are fully dry and store them at low temperature. When it is to be determined, use mechanical grinding, pass through a 40-mesh sieve, and store it at low temperature for future use. Weigh 0.2g sample (accurate to 0.0001g), and place it in a 10mL centrifuge tube. Add 5 mL of 70% methanol solution preheated at 70°C, mix well, and then bathe in 70°C water bath. Water bath for 10 minutes, and stir 2-3 times in the middle. Centrifuge at 3,500r / min for 10min, and transfer the supernatant. Repeat steps 2-4, combine the supernatants coll...

Embodiment 2

[0045] Genotype Analysis of Tea Tree Resources with Functional Marker dCAPS-F3′5′H

[0046] 1. Test materials

[0047] The materials studied in this experiment are listed in Table 2, including 28 tea tree resources.

[0048] Table 2 The 28 tea tree resources studied in this experiment and their dihydroxycatechin content

[0049]

[0050] 2. Functional markers for different tea tree resources F3'5'H Genotype detection of gene SNP848

[0051] Marker genotype analysis of 28 materials using molecular marker dCAPS-F3'5'H. DNA extraction, PCR amplification system, program, enzyme digestion and agarose gel conditions are the same as in Example 1. The result is as image 3 As shown, the genotype of materials 1-10 is AA, the genotype of materials 11-20 is AG, and the genotype of materials 21-28 is GG.

[0052] 3. Identification of catechin content in 28 tea tree materials

[0053] The method for identifying catechins by high-performance liquid chromatography is the same as t...

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PUM

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Abstract

The invention belongs to the technical field of biology, particularly relates to a flavonoid 3',5'-hydroxylase gene functional marker for screening a high-dihydroxyl catechin-content tea plant, as well as application and an application method thereof, and discloses a functional marker for identifying the high-dihydroxyl catechin-content tea plant. The functional marker is applied to molecular marker-assisted selection, and can be used for rapidly screening a tea plant material with high dihydroxyl catechin content, thereby accelerating cultivation of a high-quality team plant variety. The functional marker has great theoretical significance and high economic value for molecular marker-assisted selection of a high-dihydroxyl catechin-content tea plant variety.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a flavonoid 3', 5'-hydroxylase gene functional marker for screening high-dihydroxycatechin tea trees and its application and application method. Background technique [0002] Catechins are the main components of flavonoids in tea tree tea, accounting for 8-26% of the dry weight of green tea (Chen & Zhou, 2005). Almost all the characteristics of finished tea, such as taste, soup color and aroma, etc. Directly or indirectly related to catechins (Wang et al., 2000). Catechin has anti-oxidation, anti-mutagenesis, anti-cancer, anti-cardiovascular disease, ultraviolet radiation protection, anti-diabetes, anti-bacterial and anti-inflammatory, weight loss and treatment of Parkinson's disease (Xia Tao and Gao Liping, 2009), belonging to Huang Alkan-3-ol compounds. Catechin is a derivative of 2-phenylbenzopyran. According to the number of hydroxyl groups in the B ring of catechin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 陈亮金基强姚明哲马建强马春雷
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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