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Method for detecting quercetin through fluorescent micro-probe and application

A fluorescent microsphere, fluorescence technology, applied in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of slow response time and poor sensitivity of fluorescent probes, and improve the stability of fluorescent signals and sensing performance. , low cost, good specificity effect

Active Publication Date: 2021-12-03
SHANGHAI UNIV OF ENG SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most fluorescent probes have slow response times and poor sensitivity

Method used

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  • Method for detecting quercetin through fluorescent micro-probe and application
  • Method for detecting quercetin through fluorescent micro-probe and application
  • Method for detecting quercetin through fluorescent micro-probe and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The preparation of embodiment 1 fluorescent microsphere and fluorescent microprobe

[0043] (1) Preparation of fluorescent microspheres

[0044] In a 250mL round bottom flask, dissolve 0.10g (0.30mmol) hexachlorocyclotriphosphazene (HCCP) and 0.32g (0.90mmol) curcumin in 50mL acetonitrile until hexachlorocyclotriphosphazene and curcumin are completely dissolved in After the acetonitrile solution, 2 mL of triethylamine (about 27 mmol) was added and stirred at room temperature for 8 h. After the reaction was complete, centrifuge to obtain the lower precipitate, which was washed with ethanol and purified water until the supernatant was clear. Vacuum-dry the obtained precipitate at 50°C for 12 hours to obtain a light yellow powder with a particle size of 0.5-2 μm, which is fluorescent microspheres. The technical route is:

[0045]

[0046] (2) Preparation of fluorescent microprobe PC-Al(III)

[0047] A PC solution with a concentration of 12 mg / mL was prepared with eth...

Embodiment 2

[0050] Embodiment 2 Fluorescence-specific selectivity test

[0051]Prepare 12mg / mL PC ethanol solution and 100mg / ml Al(III) aqueous solution, add 2mL ethanol solution, 10μL PC solution and 10μL Al(III) aqueous solution to the cuvette, and use a fluorescence spectrometer to investigate the fluorescence micrometer Selectivity of probe PC-Al(III) for the small molecule quercetin.

[0052] The result is as figure 1 As shown, under the excitation condition at 372nm, the single fluorescent microprobe PC-Al(III) has weak fluorescence emission intensity at 510nm in ethanol solution, after adding quercetin, the fluorescence emission intensity at 510nm It is obviously enhanced, but when other substances are added, the fluorescence emission intensity of the solution system has no obvious change compared with the fluorescence emission intensity of the individual probe system, indicating that the fluorescent microprobe PC-Al(III) has the effect on quercetin in ethanol solution. Better fl...

Embodiment 3

[0053] Embodiment 3 anti-interference test

[0054] Prepare 12mg / mL PC ethanol solution and 100mg / mL Al(III) aqueous solution, add 2mL ethanol solution, 10μL PC solution and 10μL Al(III) solution to 26 clean fluorescent cuvettes respectively , and then add 10 μM quercetin and 100 μM other analytes, such as:

[0055] Other small molecules: cysteine ​​(Cys), histidine (His), glucose (Glu), ascorbic acid (AA), tannin (Tannin), catechol (Catechol), rutin (Rutin), soybean Daidzein, Baicalein;

[0056] Cation: Na + 、K + , Ca 2+ 、Cu 2+ ,Zn 2+ 、Cd 2+ , Sn 2+ 、Co 2+ 、Ni 2+ , Mg 2+ , Fe 3+ ;

[0057] Anion: Cl - 、ACO - 、H 2 PO 4 - 、HCO 3 - , CO 3 2- , SO 4 2- .

[0058] Detect on the fluorescence spectrometer, draw the histogram of the highest fluorescence intensity corresponding to different analytes, and obtain the fluorescence emission histogram, such as figure 2 As shown, it is proved that the recognition of quercetin in ethanol solution by the fluorescent...

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Abstract

The invention discloses a method for detecting quercetin through a fluorescent micro-probe and application. According to the invention, curcumin is taken as a target and phosphonitrilic chloride trimer is taken as a linking group to form a polycyclotriphosphazene co-curcumin fluorescent microsphere; the fluorescent micro-probe is formed on the basis of complexing of the fluorescent microsphere and an aluminum ion; and by virtue of good coordination capability of the quercetin and the metal ion, the quercetin is combined with the aluminum ion in the fluorescent micro-probe to release a fluorescence signal so as to enhance the fluorescence of a system, so rapid detection of the quercetin is realized. The fluorescent micro-probe is a polyphosphazene hyperbranched micron-sized fluorescent micro-probe, fluorescence signal stability and sensing performance under complex background interference are improved by constructing a curcumin-metal ion-quercetin micron-sized reactor, the lowest detection limit of quercetin micromolecules is 2.32 * 10<-8> mol / L, rapid response within 1 min is achieved, high sensitivity is realized, anti-interference capability is strong, identification is rapid, and a detection result is accurate.

Description

technical field [0001] The invention relates to quercetin detection technology, in particular to a quercetin detection method and application of a fluorescent micro-probe. Background technique [0002] The flavonoid quercetin (3′,3,4′,5,7-pentahydroxyflavone) widely exists in many Chinese herbal medicines and daily foods, and has anti-inflammatory, anti-oxidation, anti-tumor, and anti-cancer effects. Studies have proved that foods rich in flavonoids have important biological activities, which are beneficial to human cells by enhancing the activity of various enzymes, such as anti-inflammatory, anti-oxidation, liver protection, anti-tumor and antibacterial effects, and are also related to reducing the risk of certain cancers , the detection of quercetin is beneficial for evaluating the nutritional quality in food chemistry and the antioxidant activity of quercetin in pharmacology. [0003] In the prior art, there are many methods for detecting quercetin, such as spectrophoto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G79/025C09K11/06G01N21/64
CPCC08G79/025C09K11/06G01N21/643C09K2211/1491
Inventor 王乐苏稀琪瞿祎罗芳芳
Owner SHANGHAI UNIV OF ENG SCI
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