Epitope peptide H362 of HN protein in peste des petits ruminants virus (PPRV), and determination, preparation method and application thereof

A technology of PPR and protein antigens, applied in the fields of bioinformatics and immunology, can solve problems such as unfavorable PPR global eradication plan, vaccine thermal stability and virulence reversion

Active Publication Date: 2017-09-29
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0003] At present, the main prevention method of the disease is the attenuated vaccine immunization, but the vaccine has problems

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  • Epitope peptide H362 of HN protein in peste des petits ruminants virus (PPRV), and determination, preparation method and application thereof
  • Epitope peptide H362 of HN protein in peste des petits ruminants virus (PPRV), and determination, preparation method and application thereof
  • Epitope peptide H362 of HN protein in peste des petits ruminants virus (PPRV), and determination, preparation method and application thereof

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Embodiment Construction

[0056] A kind of Peste des petits ruminants virus HN protein epitope peptide of the present invention, the aminoacid sequence of described epitope peptide is H362: 362 EANWVVPSTDVRDL 375 .

[0057] The method for determining and preparing the antigenic epitope of the present invention comprises the following steps:

[0058] Calculation simulation:

[0059] Step 1: Use molecular simulation software to construct the 3D structure of the virtual HN protein head: Use Discovery Studio V4.5 to perform homology modeling on the two target proteins——PPRV HN of wild strain and PPRV HN protein of vaccine strain; among them, PPRVHw, GenBank The accession number is FJ905304; PPRV Hv, GenBank accession number: X74443;

[0060] Step 2, search for templates: search the PDB database (www.pdb.org), find the structure of homologous proteins resolved through experiments, and select the protein structure with a sequence identity of more than 30% and a relatively long sequence as the template str...

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Abstract

The invention relates to an epitope peptide H362 of an HN protein in PPRV, and determination, a preparation method and application thereof. The amino acid sequence of the epitope peptide is H362: <362>EANWVVPSTDVRDL<375>. The invention detects reactogenicity of a monoclonal antibody and PPRV and specificity of the monoclonal antibody; according to detection results, the monoclonal antibody has good reactogenicity to rPPRV-HN-F protein; immunoinformatic technology is cooperatively used for predicating the B cell epitope of the HN protein; an aminated ELISA plate is employed for detecting candidate epitopes and the monoclonal antibody 10E3, and the epitope peptide H362 corresponding to 10E3 is determined; and determination of the epitope peptide lays a theoretical foundation for preparation of epitope vaccine antigens and diagnostic reagent antigens for PPRV.

Description

technical field [0001] The invention belongs to the technical fields of bioinformatics and immunology, and in particular relates to an antigenic epitope peptide H362 of HN protein of Peste des petits ruminants virus and its determination, preparation method and application. Background technique [0002] Peste des petits ruminants virus (PPRV) belongs to the genus Morbillivirus and is the pathogen that causes acute infectious diseases of small ruminants such as goats and sheep. The disease is characterized by high morbidity and mortality, and is recognized by the World Organization for Animal Health (OIE) Listed as a legally notifiable animal infectious disease, my country classifies it as a Class I animal disease. Peste des petits ruminants virus was initially reported to only infect goats and sheep, but in recent years, cases of interspecies transmission of the virus have been continuously reported. At present, the disease is mainly distributed in Africa and Asia, and is thr...

Claims

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Application Information

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IPC IPC(8): C07K14/12A61K39/165A61P31/14G01N33/569G01N33/68
CPCA61K39/12A61K2039/552C07K14/005C12N2760/18322C12N2760/18334G01N33/56983G01N33/6854G01N2469/20
Inventor 窦永喜梁忠祥朱学亮蒙学莲张志东才学鹏
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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