A kind of antigenic epitope peptide of F protein of Peste des petits ruminants virus and its determination, preparation method and application

A technology of PPR and protein antigens, applied in the fields of bioinformatics and immunology, can solve problems such as unfavorable PPR global eradication plan, vaccine thermal stability and virulence reversion

Active Publication Date: 2020-09-04
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

[0003] At present, the main prevention method of the disease is attenuated vaccine immunization, but the vaccine has problems such as thermal stability and strong virulence, and is not conducive to the global eradication plan of Peste des petits ruminants. F is a glycoprotein embedded in the PPRV envelope , constituting the fibrils on the surface of virus particles, HN protein can interact with SLAM receptors on lymphocytes to mediate virus invasion, but the virus invasion process needs the help of F protein to complete, Peste des petits ruminants virus can cause strong Cellular immunity and humoral immune response, and F protein is the main protective antigen; in addition, F protein may be the main reason for the cross-protective properties of measles virus, and F protein is an important target in vaccine design and detection method establishment , so the F protein epitope mapping and antibody preparation are of great significance

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  • A kind of antigenic epitope peptide of F protein of Peste des petits ruminants virus and its determination, preparation method and application
  • A kind of antigenic epitope peptide of F protein of Peste des petits ruminants virus and its determination, preparation method and application
  • A kind of antigenic epitope peptide of F protein of Peste des petits ruminants virus and its determination, preparation method and application

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Embodiment Construction

[0083] The present invention will be described in further detail below in conjunction with the examples.

[0084] A kind of Peste des petits ruminant virus F protein epitope peptide of the present invention, the amino acid sequence of its epitope peptide is:

[0085] F176: 176 DYINNELVPSVHRMSCEL 193 (SEQ ID NO.1)

[0086] or / and F347: 347 MSPLLQECFRGSTKS 361 (SEQ ID NO.2)

[0087] or / and F391: 391 CKCYTTETVINQDPDKLL 408 (SEQ ID NO.3)

[0088] or / and F417: 417 TVINQDPDKGPVGSREYPD 435 (SEQ ID NO.4)

[0089] or / and F430: 430 SREYPDSVYLH 440 (SEQ ID NO.5)

[0090] or / and F502: 502 VSLGLVTLICCCKGRCRNK520 (SEQ ID NO. 6).

[0091] The invention uses a variety of immune informatics software to predict the B cell epitope of the target protein, and then artificially synthesizes the predicted different epitopes, and uses the indirect ELISA method to verify its reactogenicity, and different polypeptides are coated with amination A microtiter plate is used to detect the rea...

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Abstract

The invention relates to peste des petits ruminant virus F protein epitope peptide. The amino acid sequences of the epitope peptide are respectively as follows: F176: <176>DYINNELVPSVHRMSCEL<193>, or / and F347: <347>MSPLLQECFRGSTKS<361>, or / and F391: <391>CKCYTTETVINQDPDKLL<408>, or / and F417: <417>TVINQDPDKGPVGSREYPD<435>, or / and F430: <430>SREYPDSVYLH<440>, or / and F502: <502>VSLGLVTLICCCKGRCRNK<520>. B cell epitopes of a target protein are predicted, the different predicted epitopes are respectively artificially synthesized, the reactogenicity with an antibody of F protein is detected, and therefore, the B cell epitopes of the PPRV F protein are authenticated.

Description

technical field [0001] The invention belongs to the technical fields of bioinformatics and immunology, and in particular relates to an antigenic epitope peptide of F protein of Peste des petits ruminants virus and its determination, preparation method and application. Background technique [0002] Peste des petits ruminants virus (PPRV) belongs to the genus Morbillivirus and is the pathogen that causes acute infectious diseases of small ruminants such as goats and sheep. The disease is characterized by high morbidity and mortality, and is recognized by the World Organization for Animal Health (OIE) Listed as a legally notifiable animal infectious disease, my country classifies it as a Class I animal disease. Peste des petits ruminants virus was initially reported to only infect goats and sheep, but in recent years, cases of interspecies transmission of the virus have been continuously reported. At present, the disease is mainly distributed in Africa and Asia, and is threateni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/12A61K39/165A61P31/14G01N33/569G01N33/68
CPCA61K39/12A61K2039/552C07K14/005C12N2760/18322C12N2760/18334G01N33/56983G01N33/6854G01N2469/20
Inventor 窦永喜梁忠祥朱学亮蒙学莲张志东才学鹏
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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