Carbon quantum dot two-photon fluorescent dye for ultrafast cell staining

A two-photon fluorescence, carbon quantum dot technology, applied in the field of nanomaterials, can solve the problem of lack of two-photon fluorescent dyes, and achieve the effects of good biocompatibility, excellent imaging performance, and excellent imaging effect.

Pending Publication Date: 2022-07-12
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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But reliable two-photon fl

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  • Carbon quantum dot two-photon fluorescent dye for ultrafast cell staining
  • Carbon quantum dot two-photon fluorescent dye for ultrafast cell staining
  • Carbon quantum dot two-photon fluorescent dye for ultrafast cell staining

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Example Embodiment

[0034] Example 1

[0035] A carbon quantum dot two-photon fluorescent dye for ultrafast cell dyeing in this embodiment is prepared by the following method:

[0036] 1) Prepare carbon quantum solution by one-step solvothermal method with m-phenylenediamine as precursor:

[0037] 0.2 g of m-phenylenediamine was added to 20 mL of absolute ethanol, then 672 μL of concentrated hydrochloric acid (mass concentration: 36.5%) was added, and the obtained mixture was subjected to hydrothermal treatment at 180° C. in a polytetrafluoroethylene-lined stainless steel autoclave. After 2 hours, the reaction was cooled to room temperature, and the product was centrifuged at 10,000 rpm for 20 minutes to remove non-fluorescent precipitates to obtain a carbon quantum solution;

[0038] 2) Using silica gel column chromatography to separate the green fluorescent component from the carbon quantum prepared in step 1), which is the carbon quantum dot two-photon fluorescent dye:

[0039] A mixture of ...

Example Embodiment

[0041] Example 2

[0042] The relevant performance test of the carbon quantum dot two-photon fluorescent dye prepared in Example 1 was carried out, and the following results were obtained.

[0043] 1. Refer to figure 1 , is the transmission electron microscope (TEM) photo of the carbon quantum dot two-photon fluorescent dye, it can be seen that the carbon dot is spherical, the lattice is obvious, and the lattice spacing is 0.21 nm.

[0044] 2. Refer to figure 2 , is the X-ray diffraction pattern of the carbon quantum dot two-photon fluorescent dye. It can be seen that the X-ray diffraction 2θ angle of the carbon dot is 22°, which is consistent with the TEM.

[0045] 3. Refer to image 3 , is the UV absorption spectrum and fluorescence spectrum of the carbon quantum dot two-photon fluorescent dye. It can be seen from the figure that it has the largest absorption band at 248 nm, which represents the π-π* transition of the aromatic C=C bond ; the weak absorption peak at about ...

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Abstract

The invention discloses a carbon quantum dot two-photon fluorescent dye for ultrafast cell staining. The carbon quantum dot two-photon fluorescent dye is prepared by the following steps: 1) preparing a carbon quantum solution by taking m-phenylenediamine as a precursor and adopting a one-step solvothermal method; and 2) separating a green fluorescent component from the carbon quantum prepared in the step 1) by adopting silica gel column chromatography, namely the carbon quantum dot two-photon fluorescent dye. The absolute quantum efficiency of the carbon quantum dot two-photon fluorescent dye for ultrafast cell staining provided by the invention reaches up to 58.65%, and the carbon quantum dot two-photon fluorescent dye shows good biocompatibility and shows an excellent imaging effect even at a low concentration; the fluorescent probe can enter cells within 10 seconds, so that a solid foundation is provided for rapid fluorescence imaging; in addition, the G-CDs can also be used for two-photon imaging, a near-infrared excitation light source is used, and the G-CDs also shows strong green fluorescence and shows excellent imaging performance in 4T1 cells.

Description

technical field [0001] The invention relates to the field of nanomaterials, in particular to a carbon quantum dot two-photon fluorescent dye used for ultrafast cell dyeing. Background technique [0002] Bioimaging is an important research method to understand the tissue structure of organisms and elucidate various physiological functions of organisms. Due to the advantages of high sensitivity, high resolution, intuitive imaging, fast imaging speed, and non-destructive testing, biological imaging has been widely used in scientific research and biomedical diagnosis. Bioimaging also has important practical application value in exploring the pathogenesis, clinical manifestations and genetic lesions of diseases, understanding the corresponding physiological and pathological information, diagnosing diseases, and developing new medical methods. Currently, biological imaging includes fluorescence imaging, magnetic resonance imaging (MRI), computed tomography (CT), emission computed...

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Application Information

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IPC IPC(8): C09K11/65B82Y20/00B82Y40/00G01N21/64
CPCC09K11/65B82Y20/00B82Y40/00G01N21/6428
Inventor 李力朱彤彤梅茜葛明锋常智敏董文飞
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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