Peste des petits ruminant virus HN protein epitope peptide as well as determination and preparation method and application thereof

Abstract

The invention relates to peste des petits ruminant virus HN protein epitope peptide. The amino acid sequences of the epitope peptide are as follows: H123: <123>KFLNPDREYDFRDLR<137>, or/and H185: <185>GTGCLGRTVTRA<196>, or/and H487: <487>IRGPRGRCH<495>, or/and H569: <569>ECFPWYHKVWCYHDCLI<585>. B cell epitopes of a target protein are predicted by virtue of multiple immunoinformatic software, the different predicted epitopes are respectively artificially synthesized, the reactogenicity of the predicted epitopes is verified by virtue of an indirect ELISA method, an aminated ELISA Plate is coated with different polypeptides, and the reactogenicity with an antibody of HN protein is detected, and therefore, the B cell epitopes of the PPRV HN protein are authenticated.

Description

technical field [0001] The invention belongs to the technical fields of bioinformatics and immunology, and in particular relates to an epitope peptide of HN protein antigen of Peste des petits ruminants virus and its determination, preparation method and application. Background technique [0002] Peste des petits ruminants virus (PPRV) belongs to the genus Morbillivirus and is the pathogen that causes acute infectious diseases of small ruminants such as goats and sheep. The disease is characterized by high morbidity and mortality, and is recognized by the World Organization for Animal Health (OIE) Listed as a legally notifiable animal infectious disease, my country classifies it as a Class I animal disease. Peste des petits ruminants virus was initially reported to only infect goats and sheep, but in recent years, cases of interspecies transmission of the virus have been continuously reported. At present, the disease is mainly distributed in Africa and Asia, and is threatenin...

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Problems solved by technology

[0003] At present, the main prevention method of the disease is attenuated vaccine immunization, but the vaccine has problems such as thermal stability and strong virulence, and is not conducive to the global eradication plan of Peste des petits ruminants. HN is a glycoprotein embedded in the PPRV envelope , constituting the fibrils on the surface of virus particles, HN protein can interact with SLAM receptors on lymphocytes to mediate virus invasion, so the HN protein determines the host tropism; On cells, it may be the main reason for the immune suppression caused by Peste des petits ruminants virus. Peste des pets ruminants virus can cause strong cellular and humoral immune responses in the body, and HN protein is the main protective antigen. HN protein is the vaccine design and An important target established by the detection method, so the mapping of the epitope map of the HN protein and the preparation of antibodies are of great significance

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