Detection method for bovine FRAS1 gene insertion/deletion mutation and application thereof

A detection method and deletion mutation technology, which can be used in the determination/inspection of microorganisms, biochemical equipment and methods, etc., and can solve the problems such as FRAS1 gene polymorphism and bovine reproductive traits, and little research and application of InDel markers. , to achieve the effect of improving the breeding speed and low cost of improved varieties

Active Publication Date: 2021-05-14
NORTHWEST A & F UNIV
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Problems solved by technology

And compared with other variations, InDel polymorphisms are easier to be directly identified by PCR amplification and agarose gel electrophoresis, but as genetic identification markers, the research

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  • Detection method for bovine FRAS1 gene insertion/deletion mutation and application thereof
  • Detection method for bovine FRAS1 gene insertion/deletion mutation and application thereof
  • Detection method for bovine FRAS1 gene insertion/deletion mutation and application thereof

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[0022] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments. It should be noted that this embodiment is only used to explain the present invention, but not to limit the protection scope of the present invention.

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Abstract

The invention discloses a detection method for bovine FRAS1 gene insertion/deletion mutation and application thereof. Bovine whole genome DNA is used as a template, a part of fragments of the bovine FRAS1 gene are amplified through PCR, and agarose gel electrophoresis is applied to identify the genotype of 15-bp deletion mutation sites of the individual FRAS1 gene. According to a character correlation analysis result, the genotype of the 15-bp deletion mutation site of the FRAS1 gene is remarkably related to the breeding character of the cow. Therefore, the FRAS1 gene 15-bp deletion mutation site can be used for early marker-assisted selection of cow reproductive traits so as to accelerate establishment of cow populations with excellent genetic resources.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and livestock breeding, and relates to the detection and application of bovine FRAS1 gene insertion / deletion (InDel) polymorphism. Background technique [0002] Improving the fertility of female livestock is the most direct and effective way to make the cattle farming industry profitable. However, with the continuous artificial selection of high-yielding dairy cows, the female fertility gradually decreased synchronously. Therefore, increasing the reproductive performance of cows is an urgent problem to be solved. [0003] The ovary is the source of the cow's gonads and germ cells, such as oocytes. Importantly, the development and morphology of ovaries are closely related to the reproductive performance of cows. The corpus luteum in the ovary is a "temporary gland" whose main task is to synthesize and secrete progesterone, or further atrophy and degenerate into a white body. Progesterone i...

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2600/124C12Q2531/113C12Q2565/125
Inventor 蓝贤勇李洁宋恩亮赵海谕姜富贵潘传英赵佳宁费攀锋王勇胜陈宏
Owner NORTHWEST A & F UNIV
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