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A kind of gene and amino acid technology, applied in the application field of Arabidopsis thaliana gene FERONIA in plant response to amino acids
Active Publication Date: 2020-06-12
HUNAN UNIV
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Embodiment 1
[0033] The Arabidopsis wild-type Col-0 and mutant fer-4 (seeds are all derived from ABRC seed bank) seeds were sterilized with 75% alcohol for 5 min, rinsed with sterile water twice, then sterilized with 15% sodium hypochlorite for 5 min, sterile Rinse with water 4 times. Put the sterilized seeds at 4°C and vernalize for 3 days. The vernalized seeds were planted on normal 1 / 2MS medium, inorganic nitrogen-deficient 1 / 2MS medium, and inorganic nitrogen-deficient 1 / 2MS solid medium containing 3mM 16 kinds of amino acids respectively, and placed horizontally at 22°C for a long time. Grow in the sunlight incubator for 10 days, take pictures (see figure 1 ). (16 kinds of amino acids are: alanine (Ala), arginine (Arg), asparagine (Asn), aspartic acid (Asp), cysteine (Cys), glutamine (Gln) , glutamic acid (Glu), glycine (Gly), histidine (His), isoleucine (Ile), leucine (Leu), lysine (Lys), methionine (Met), proline (Pro), threonine (Thr), valine (Val).)
[0034] Table 1 Inorgan...
Embodiment 2
[0048] The Arabidopsis wild-type Col-0 and mutant fer-4 (derived from ABRC) seeds were sterilized with 75% alcohol for 5 minutes, washed twice with sterile water, then sterilized with 15% sodium hypochlorite for 5 minutes, and washed 4 times with sterile water . Put the sterilized seeds at 4°C and vernalize for 3 days. Plant the vernalized seeds in 1 / 50 nitrogen-free th B5 medium, after 4 days, the seedlings were transplanted into nitrogen-free 1 / 50 medium containing 0.5mM aspartic acid (Asp), glutamine (Gln) and glycine (Gly) respectively. th In the B5 medium, vertically placed in a long-day incubator at 22°C for 10 days, and the root length was counted.
[0049] B5 medium formula:
[0050]
[0051] Prepare 1 / 50 th In the B5 medium, the concentration of nutrient elements (macroelements, trace elements and iron salts) used is 1 / 50 of the normal B5 medium, the sucrose concentration is 0.5%, and the phytogel is 1%. Then add the final concentration of 1mM MgCl 2 and Ca...
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Abstract
The invention discloses an application of a gene FERONIA, and belongs to the technical field of gene regulation and control. The invention discloses a method for adding amino acid into a nitrogen-deficient culture medium. Wild type arabidopsis thaliana Col-0 and FERONIA gene deletion mutants fer-4 are dibbled on a culture medium of the arabidopsis thaliana; amino acid capable of restoring the nitrogen deficiency phenotype of wild arabidopsis thaliana is found, but the nitrogen deficiency phenotype of the arabidopsis thaliana fer-4 mutant cannot be restored or only part of the amino acid can berestored, and it is shown that FERONIA participates in the utilization process of organic nitrogen by plants, that is, the FERONIA gene of arabidopsis thaliana participates in the response process ofplant roots. The invention provides a theoretical and method basis for functional research of the FERONIA gene. The FERONIA disclosed by the invention can provide theoretical basis and genetic resources for cultivating new crop varieties.
Description
Technical field: [0001] The invention belongs to the technical field of gene regulation, and in particular relates to the application of Arabidopsis gene FERONIA in plant response amino acids. Background technique: [0002] Nitrogen is one of the most important macroelements for plant growth and development. It is not only an important component of biological macromolecules such as proteins, nucleic acids, and phospholipids, but also participates in the formation of important compounds such as enzymes, chlorophyll, and plant hormones. The nitrogen used by plants mainly comes from soluble organic nitrogen compounds and inorganic nitrogen compounds in soil or water. [0003] In recent years, as the most important member of the subfamily CrRLK1L, the receptor protein kinase FERONIA (abbreviated as FER, the same below) and its signaling network have received a lot of attention. Previous studies have shown that the cell membrane receptor protein kinase FERONIA signaling pathway ...
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