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Screening method of feed probiotics capable of preventing porcine salmonella infection

A Salmonella, screening method technology, applied in biochemical equipment and methods, resistance to vector-borne diseases, determination/inspection of microorganisms, etc. To achieve the effect of saving manpower, avoiding discomfort, and avoiding blindness

Inactive Publication Date: 2018-04-10
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although a large number of probiotics have been screened in my country and widely used in production practice, these probiotics are mainly used to promote the digestion and absorption of nutrients in the intestinal tract of animals and promote growth, and many probiotics do not come from the body. Animals, after entering the body of the host, it is difficult to adapt to the host, and it is difficult to exert its exact effect
Moreover, the existing probiotic screening technologies mainly include in vitro antibacterial methods and in vivo bacterial attack methods, which have the disadvantages of blindness, low efficiency, poor pertinence, and mostly exogenous bacteria.

Method used

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  • Screening method of feed probiotics capable of preventing porcine salmonella infection
  • Screening method of feed probiotics capable of preventing porcine salmonella infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] (1) Select 5 pig farms where Salmonella infection broke out, and observe the typical clinical symptoms of piglets with abdominal pain and diarrhea, high fever and dehydration, flatulence, nausea and vomiting, dyspnea, sepsis, purpura under the abdomen and chest of the ears and even death, and the piglets Necropsy of pathological lesions with undigested food and a small amount of mucus mixed in feces, mesenteric pus and blood, lymph node edema, and small intestinal villus shrinkage, combined with qualitative PCR detection of Salmonella, from the group of piglets that were positive in qualitative PCR detection, select piglets with typical 25 diseased piglets with clinical symptoms and obvious pathological damage and 25 disease-resistant piglets without typical clinical symptoms and obvious pathological damage; each intestinal digesta sample was collected from the diseased piglets and resistant piglets, and stored at -80°C for later use .

[0085] (2) Extract the genomes o...

Embodiment 2

[0091] (1) Forty 3-day-old Salmonella-negative piglets purchased from pig farms that had not been immunized against Salmonella were fed with artificial milk replacer as test piglets, and were randomly divided into groups Ⅰ, Ⅱ, Ⅲ and Ⅳ group, 10 piglets in each group, wherein, group Ⅰ is the control group, group Ⅱ, group Ⅲ and group Ⅳ are probiotic groups;

[0092] (2) After the probiotics (including Lactobacillus pentosus and Clostridium butyricum) screened in Example 1 were cultured in vitro, the test piglets were inoculated with probiotics from the morning of the 3rd day of age in a 10-fold increasing manner. bacteria, until the test piglets grow to 7 days old, the inoculation dose on the first day is 5×10 2 cfu / 100uL, and then the subsequent daily inoculation doses were 5×10 3 cfu / 100uL, 5×10 4 cfu / 100uL, 5×10 5 cfu / 100uL, 5×10 6 cfu / 100uL; Among them, piglets in group I were inoculated with 100uL of normal saline, piglets in group II were inoculated with Lactobacillus ...

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Abstract

The invention discloses a screening method of feed probiotics capable of preventing porcine salmonella infection. The screening method comprises the following steps: screening piglets infected by salmonella and disease resistant piglets, collecting intestinal content samples of piglets infected by salmonella and disease resistant piglets; carrying out high-throughput sequencing on intestinal microbial floras of piglets infected by salmonella and disease resistant piglets; performing meta-genomic comparison and analysis on intestinal microbes of piglets infected by salmonella and disease resistant piglets; and screening and identifying probiotics capable of preventing salmonella infection. The provided screening method screens out probiotics capable of preventing salmonella infection from agreat amount of microbial floras of disease resistant piglets, and is strongly targeted, the blindness of screening is avoided, the screening efficiency is improved, and a large amount of labor, materials, and time is saved.

Description

technical field [0001] The invention relates to the technical field of feed additives for piglets, in particular to a screening method for feeding probiotics resistant to Salmonella infection in pigs. Background technique [0002] Probiotics are playing an increasingly important role in safeguarding human and animal health or preventing certain diseases, especially under the premise that antibiotics will be completely banned in my country, intestinal health and diarrhea will be bottlenecks that threaten the development of animal husbandry in my country. Among them, salmonellosis, also known as paratyphoid fever, is a general term for various animal diseases caused by Salmonella bacteria. Clinically, it is often manifested as sepsis and enteritis, and it can also cause abortion in pregnant female animals. Therefore, research and development of new antibiotic substitutes, screening of feed probiotics with specific effects such as prevention and treatment of animal salmonellosi...

Claims

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Application Information

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IPC IPC(8): C12Q1/6869C12Q1/04
CPCC12Q1/6869C12Q2535/122C12Q2537/165Y02A50/30
Inventor 吴涛侯永清丁斌鹰易丹赵迪
Owner WUHAN POLYTECHNIC UNIVERSITY
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