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Product for preventing and/or treating salmonella infection

A Salmonella, product technology, applied in the field of biomedicine, can solve the problems of increased antibiotic content, unsatisfactory effect, intestinal dysfunction, etc., to achieve strong effect, strong effect of prevention and/or treatment of Salmonella infection

Active Publication Date: 2020-03-06
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, antibiotics are the main drugs used clinically to treat Salmonella infection. However, the use of antibiotics to treat Salmonella infection will kill a large number of intestinal commensal bacteria that are beneficial to the human body, causing side effects such as intestinal dysfunction; and , long-term use of antibiotics leads to Salmonella resistance to commonly used antibiotics, and the emergence of multi-drug resistant Salmonella has become a common phenomenon; in addition, excessive use of antibiotics will increase the amount of antibiotics in the environment, resulting in ecological imbalance
In addition to antibiotics, some traditional Chinese medicine formulas can also relieve symptoms after infection, but the effect is not ideal

Method used

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  • Product for preventing and/or treating salmonella infection
  • Product for preventing and/or treating salmonella infection
  • Product for preventing and/or treating salmonella infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1: The effect of eugenol on the growth of Lactobacillus plantarum ZS2058 and Salmonella

[0059] The bacterial suspension of Lactobacillus plantarum ZS2058 and the bacterial suspension of Salmonella were used MRS - Dilute the medium to a concentration of 2×10 6 CFU / mL, get the dilution of Lactobacillus plantarum ZS2058 and Salmonella; add the concentration of 0mmol / L, 0.0125mmol / L, 0.025mmol / to the dilution of Lactobacillus plantarum ZS2058 and Salmonella respectively. L, 0.05mmol / L, 0.1mmol / L, 0.2mmol / L, 0.4mmol / L, 0.8mmol / L, 1.6mmol / L, 3.2mmol / L, 6.4mmol / L, 12.8mmol / L eugenol, Incubate statically at 37°C. During the incubation period, measure the OD of the diluted solution with a microplate reader every 1 hour during the first 8 hours. 600 , Measure the OD of the diluent every 2h at 8-12h 600 To detect the growth curve of Lactobacillus plantarum ZS2058 and Salmonella under the influence of different concentrations of eugenol, each group has 4 parallel (test resul...

Embodiment 2

[0063] Example 2: Effect of Eugenol and / or Lactobacillus plantarum ZS2058 on Salmonella adhesion, invasion ability and virulence gene expression

[0064] Use RMPI-1640 medium containing 5% (v / v) CO 2 Cultivate HT-29 cells in a 37°C incubator until the cell density of HT-29 cells reaches 80%, and transfer to a 6-well plate to obtain a well plate full of HT-29 cells; transfer a well plate full of HT-29 cells Divided into four groups, namely Ctrl group, eugenol group (E), Lactobacillus plantarum ZS2058 group (Z) and Lactobacillus plantarum ZS2058 combined with eugenol group (ZE), among which, 1× is added to the well plate of the Ctrl group 10 9 CFU / well of Salmonella, add 0.2mmol / L eugenol and 1×10 to the well plate of eugenol group (E) 9 CFU / well of Salmonella, add 1×10 to the well plate of Lactobacillus plantarum ZS2058 group (Z) 9 CFU / hole of Lactobacillus plantarum ZS2058 and 1×10 9 CFU / well of Salmonella, add 0.2mmol / L eugenol, 1×10 to the well plate of Lactobacillus plantarum Z...

Embodiment 3

[0068] Example 3: The effect of eugenol and / or Lactobacillus plantarum ZS2058 on the survival rate of mice infected with Salmonella

[0069] Thirty 7-week-old female C57BL / 6 mice were reared at a room temperature of 20~26℃, a humidity of 40~70%, 12h / 12h alternate day and night, free eating and drinking, and adaptive feeding for 1 week. Divided into 5 groups, each with 6 animals. The 5 groups are blank control group (C), model group (M), eugenol group (E), Lactobacillus plantarum ZS2058 group (Z), and Lactobacillus plantarum ZS2058 combined with eugenol Group (ZE).

[0070] Dilute the Salmonella suspension with PBS buffer to a concentration of 1×10 7 CFU / mL to obtain Salmonella dilution; Dilute Lactobacillus plantarum ZS2058 with PBS buffer to a concentration of 1×10 7 CFU / mL to obtain Lactobacillus plantarum ZS2058 dilution; dilute eugenol with PBS buffer to a concentration of 300 mg / mL to obtain eugenol dilution.

[0071] The experiment was 25 days in total: the blank control group...

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Abstract

The invention relates to a product for preventing and / or treating salmonella infection, which belongs to the technical field of biological medicines. The product provided by the invention can inhibitthe growth of salmonella; the growth amount of salmonella is reduced by 90.4 percent; according to the product disclosed by the invention, the adhesion and invasion capabilities of salmonella can be reduced; the salmonella adhered to and invading HT-29 cells is obviously reduced; moreover, the product provided by the invention can inhibit the expression of salmonella virulence genes; the expression quantities of salmonella virulence genes InvA, AvrA, SsrB, HilA and SopD are respectively reduced by 69.3 percent, 62.4 percent, 57.4 percent, 53.4 percent and 31.3 percent; the inhibition effect isobviously superior to that of single use of ZS2058 or eugenol, so that the product provided by the invention has a strong effect of preventing and / or treating salmonella infection.

Description

Technical field [0001] The invention relates to a product that can be used to prevent and / or treat Salmonella infection, belonging to the technical field of biomedicine. Background technique [0002] Salmonella belongs to the Enterobacteriaceae family. It is a global zoonotic pathogen and an important food-borne pathogen. The incubation period of salmonella infection is generally 12 to 36 hours. Salmonella caused by it ( Salmonellosis) clinical manifestations include abdominal cramps, diarrhea, fever, vomiting, gastroenteritis, etc., and can even cause sepsis or death of newborns in severe cases. [0003] Salmonella has a wide range of transmission vectors. Food-borne infections include water contaminated with Salmonella, fresh or processed meat, dairy, eggs, shellfish, vegetables, etc. The number of people infected with Salmonella in developing countries is about 20 million each year. People in developed countries in Europe and the United States are also troubled by Salmonella. T...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61P31/04A23L33/135A61K31/085
CPCA61K35/747A61K31/085A61P31/04A23L33/135A23V2002/00A23V2400/169A61K2300/00A23V2200/30Y02A50/30
Inventor 顾震南宋凡芬刘俊生黄宏萱陈卫张灏
Owner JIANGNAN UNIV
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