Caenorhabditis elegans infection model for high-throughput rapid detection of salmonella virulence and preparation method and application thereof

Abstract

The invention discloses a caenorhabditis elegans infection model for high-throughput rapid detection of salmonella virulence and a preparation method and application thereof, and belongs to the technical field of biology. The model is characterized in that: the caenorhabditis elegans is sek-1, glp-1 gene defect type caenorhabditis elegans; the culture medium for the salmonella infected caenorhabditis elegans is an S-medium buffer solution with a pH value of 7.0 + / -0.2; and the infection concentration of the salmonella is 10 < 7 >-10 < 11 > CFU / mL. The survival rates of the nematodes are obviously different when the nematodes are infected at the second to the fourth time, and the pathogenicity is preliminarily judged. Therefore, by establishing a salmonella infected caenorhabditis elegans model, the salmonella virulence can be quickly, simply, conveniently and efficiently detected, and a theoretical basis is provided for salmonella pathogenesis research and related drug screening.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Caenorhabditis elegans infection model for high-throughput rapid detection of Salmonella virulence, a preparation method and application thereof. Background technique [0002] Salmonella is the pathogen of salmonellosis, belonging to Enterobacteriaceae, Gram-negative enterobacteriaceae, there are nearly a thousand species. Bacterial size (0.6-0.9) × (1-3) μm, no spores, generally no capsule, except for Salmonella pullorum and Salmonella gallinarum, most of them have flagella all over the body. Nutritional requirements are not high, and intestinal selection and identification medium are often used for isolation and culture. The biochemical reaction has important reference significance for the identification of this bacteria. Salmonella has strong pathogenicity and is one of the important pathogens causing zoonotic diseases, including bacteria that cause food poisoning, gastroenter...

AI Technical Summary

Problems solved by technology

[0004] At present, most of the C. elegans infection models use the classic solid plate nematode killing method, but the solid plate nematode killing method has many shortcomings: high cost, time-consuming and laborious; nematodes will avoid pathogenic bacteria according to the different odors of pathogenic bacteria However, if they refuse to eat, the purpose of infection cannot be achieved; the number of nematodes that climb to the wall of the plate to avoid pathogenic bacteria is not included in the lethality of pathogenic bacteria, resulting in experimental errors.

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