Listeria monocytogenes monoclonal antibody hybridoma cell strain and application thereof

A technology for Listeria monocytogenes and hybridoma cell lines, which is applied in the directions of antibacterial immunoglobulins, biochemical equipment and methods, instruments, etc., can solve the problems of high detection cost, dependence, and high requirements for operators

CN104178458AActive Publication Date: 2014-12-03无锡迪腾敏生物科技有限公司 +1

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Patent Information

Authority / Receiving Office: CN · China
Current Assignee / Owner: 无锡迪腾敏生物科技有限公司
Publication Date: 2014-12-03

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Abstract

The invention relates to a Listeria monocytogenes monoclonal antibody hybridoma cell strain and an application thereof and belongs to the technical field of food safety immunological detection. According to the invention, a complete antigen of a specific polypeptide of Listeria monocytogenes is uniformly mixed with an equal volume of Freund adjuvant (Sigma), BALB / c mice are immunized by subcutaneous injection, after the BALB / c mice are immunized for four times, spleen cells of immunized mice are fused with mice myeloma cells of immunized mice by virtue of a PEG method, an indirect ELISA screening and three times of subcloning are carried out to obtain a monoclonal cell strain A. The monoclonal antibody secreted by virtue of the monoclonal cell strain A has cross reaction with different Listeria monocytogenes culture supernatants and no cross reaction with listeria except Listeria monocytogenes, E. coliO 157, Salmonella and Campylobacter jejuni and thus the Listeria monocytogenes monoclonal antibody hybridoma cell strain can be used in the specific detection of Listeria monocytogenes in a food.

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Description

technical field

[0001] The present invention relates to a Listeria monocytogenes monoclonal antibody hybridoma cell line and its application, in particular to the monoclonal cell line A and the application of the Listeria monocytogenes monoclonal antibody it produces, which belongs to food safety immunology detection technology field. Background technique

[0002] Listeria monocytogenes (L.monocytohenes) is a Gram-positive bacterium that widely exists in the environment and is an important zoonotic pathogen in the world. Listeria monocytogenes is a type of Listeria. Other Listeria include L.iuanuii, L.innocua, L.welshimeri ), L.seeligeri, L.grayi, L.murrayi, but L.monocytogenes is the only Listeria that causes human disease bacteria.

[0003] The susceptible population of Listeria monocytogenes is mainly children, the elderly, pregnant women, and patients with chronic diseases with poor immunity. After infection, the main manifestations are sepsis, meningitis, and mononuc...

Claims

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