Culture medium for enriching salmonella, shigella and staphylococcus aureus in composite way and preparation method thereof

A Shigella, Salmonella technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of unsuitable high background microorganisms, lack of inhibitors, etc.

Inactive Publication Date: 2015-06-03
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Universal enrichment media such as nutrient broth can amplify a variety of bacteria at the same time, but lack inhibitors to select target bacteria, and are not suitable for raw materials with high background microorganisms and untreated samples. , Shigella, and Staphylococcus aureus for enrichment while inhibiting other non-target bacteria has become a current necessity

Method used

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  • Culture medium for enriching salmonella, shigella and staphylococcus aureus in composite way and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Weigh tryptone 15g, soybean peptone 5g, sodium dihydrogen phosphate 2.5g, glucose 2.5g, sodium chloride 35g, lithium chloride 0.5g, ox bile salt 0.1g, mannitol 2g, add distilled water to 990ml, heat to dissolve, After cooling to room temperature, correct the acidity with 1mol / L NaOH to adjust the pH value to 7.2; mix well, and sterilize at 121°C for 15 minutes; weigh 0.3mg of potassium tellurite and add it to 10mL of sterilized distilled water to prepare a Potassium tellurate solution. Under sterile conditions, 10 mL of potassium tellurite solution was added; the aliquots were stored at 4°C for later use, and a culture medium for compound bacterial growth of Salmonella, Shigella and Staphylococcus aureus was prepared.

[0022] The bacteria concentration is 10 9 Salmonella, Shigella, Staphylococcus aureus and miscellaneous bacteria (see the ten species in Table 1) culture solution of cfu / mL were diluted 10 times respectively. 5 Then take 0.01mL and insert into 10mL pre...

Embodiment 2

[0027] Weigh tryptone 15g, soybean peptone 5g, sodium dihydrogen phosphate 2.5g, glucose 2.5g, sodium chloride 30g, lithium chloride 1g, ox bile salt 0.07g, mannitol 2.5g, add distilled water to 990ml, heat to dissolve, After cooling to room temperature, correct the acidity with 1mol / L NaOH to adjust the pH value to 7.2; mix well, and sterilize at 121°C for 15 minutes; weigh 0.25mg of potassium tellurite and add it to 10mL of sterilized distilled water to form a Potassium tellurate solution. Under sterile conditions, 10 mL of potassium tellurite solution was added; the aliquots were stored at 4°C for later use, and a culture medium for compound bacterial growth of Salmonella, Shigella and Staphylococcus aureus was prepared.

[0028] The bacteria concentration is 10 9 Salmonella, Shigella, Staphylococcus aureus and miscellaneous bacteria (see the ten species in Table 2) culture solution of cfu / mL were diluted 10 times respectively. 5 Then take 0.01mL and insert into 10mL prep...

Embodiment 3

[0033] Weigh tryptone 15g, soybean peptone 5g, sodium dihydrogen phosphate 2.5g, glucose 2.5g, sodium chloride 25g, lithium chloride 0.8g, ox bile salt 0.1g, mannitol 3g, add distilled water to 990ml, heat to dissolve, After cooling to room temperature, correct the acidity with 1mol / L NaOH, adjust the pH value to 7.2; mix well, sterilize at 121°C for 15 minutes; Potassium tellurate solution. Under sterile conditions, 10 mL of potassium tellurite solution was added; the aliquots were stored at 4°C for later use, and a culture medium for compound bacterial growth of Salmonella, Shigella and Staphylococcus aureus was prepared.

[0034] The bacteria concentration is 10 9 Salmonella, Shigella, Staphylococcus aureus and miscellaneous bacteria (see the ten species in Table 3) culture solution of cfu / mL were diluted 10 times respectively. 5Then take 0.01mL and insert into 10mL prepared culture medium for Salmonella, Shigella and Staphylococcus aureus, so that the bacterial concentra...

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Abstract

The invention discloses a culture medium for enriching salmonella, shigella and staphylococcus aureus in a composite way and a preparation method thereof. The culture medium comprises 13-16 parts of tryptone, 4-7 parts of soy peptone, 2-3 parts of monosodium orthophosphate, 2-3 parts of glucose, 1,000 parts of distilled water, 0.07-0.13 part of bile salt, 25-40 parts of sodium chloride, 0.4-1 part of lithium chloride, 1.5-3.5 parts of mannite and 0.0002-0.0004 part of potassium tellurite, and the pH of the culture medium is 7.1-7.3. The culture medium can be used for restraining the growth of other pathogenic microorganisms while enriching three target pathogenic bacteria simultaneously, can be directly applied to separate culturing of target bacteria and biological assay experiments, and can be directly applied to a detection technology for a plurality of pathogenic bacteria based on one detection platform such as multiple PCRs (Polymerase Chain Reactions) and the like for making a diagnosis report.

Description

technical field [0001] The invention belongs to a pre-enrichment culture medium for pathogenic bacteria, in particular to a culture medium for compound enrichment of Salmonella, Shigella and Staphylococcus aureus at the same time and a preparation method thereof. technical background [0002] Globally, the number of deaths from gastrointestinal diseases caused by Salmonella and Shigella infection is as high as one million every year, which brings a large burden to all countries. Staphylococcus aureus can not only contaminate food, but also is one of the main sources of clinical patient infection. my country's survey shows that Salmonella, Shigella and Staphylococcus aureus are among the foodborne pathogens with the highest infection rate, among which Salmonella accounts for the largest proportion of the entire infection cases. [0003] Gene-based detection methods have now been widely used and become one of the main methods for detecting foodborne pathogens in the world. T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/42C12R1/445C12R1/01
Inventor 余以刚肖性龙王永志吴晖
Owner SOUTH CHINA UNIV OF TECH
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