Compound preparation for preventing necrotic enteritis of broilers and application of compound preparation

A technology of necrotic enteritis and compound preparations, which is applied in the field of microecology, can solve the problems of reduced number of beneficial bacteria in chicken intestines, enhanced drug resistance of pathogens, and decreased production performance of chickens, so as to improve the body's immunity and reduce the incidence of disease and mortality effects

Inactive Publication Date: 2016-07-20
QINGDAO GENYUAN BIOLOGICAL TECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional prevention and treatment method is to add antibiotics to drinking water or feed, but excessive reliance on antibiotics will lead to increased drug resistance of bacteria, reduced the number of beneficial bacteria in the intestinal tract of chickens, and then caused a decline in chicken production performance

Method used

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  • Compound preparation for preventing necrotic enteritis of broilers and application of compound preparation
  • Compound preparation for preventing necrotic enteritis of broilers and application of compound preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, the screening of Lactobacillus salivarius

[0019] 1. Isolation and screening of Lactobacillus salivarius XJP2

[0020] Taking healthy broiler chickens as samples, scrape the cecal mucosa with sterile glass slides and put it in sterile water, shake and mix well for gradient dilution, pour the MRS medium into the culture and screen the colonies with obvious "calcium dissolution circle", and obtain a single colony after multiple purifications , name it XJP2, and save it.

[0021] The colony morphology of described bacterial strain XJP2 on MRS plate is as follows figure 1 As shown, its biological characteristics: Gram-positive, short rod-shaped under the microscope; the colony is round, smooth and flat, with neat edges, moist, and milky white. Physiological and biochemical indicators showed that the strain XJP2 could not reduce nitrate, liquefy gelatin, produce indole and hydrogen sulfide, and the contact enzyme test was negative.

[0022] 2. Determination...

Embodiment 2

[0025] Embodiment 2, the preparation of described composite preparation

[0026] The compound preparation for preventing broiler necrotic enteritis described in the present invention comprises the following components: the bacterial content is 2.3×10 9 The content of butyric acid bacteria and bacteria in CFU / g is 5.7×10 9 CFU / g Lactobacillus salivarius, garlic extract (10:1) accounting for 10% by mass of the compound preparation, mulberry leaf extract (10:1) accounting for 5% by mass of the compound preparation and carrier, the carrier is corncob powder . The garlic extract (10:1) and mulberry leaf extract (10:1) are commercially available.

Embodiment 3

[0027] The bacteriostasis of embodiment 3, described composite preparation

[0028] After taking 20 g of the compound preparation and soaking it in sterile water for 30 min, it was centrifuged to get the supernatant to measure its inhibitory effect on Escherichia coli, Clostridium perfringens and Salmonella. Use 96-well plates to dilute the complex, add the corresponding bacterial solution (about 10 6 CFU / mL) were incubated at 37°C for 16 hours, and the OD630nm was measured with a microplate reader. From figure 1 It can be seen that the compound has good inhibitory effect on three kinds of pathogenic bacteria, and the dilution factor increases, and the antibacterial ability decreases.

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Abstract

The invention provides a compound preparation for preventing necrotic enteritis of broilers and application of the compound preparation. Different bacterial strains and carriers are compounded with one another to obtain the compound preparation. The compound preparation comprises lactobacillus salivarius, garlic extract and folium mori . The content of butyric acid bacteria in the butyric acid bacteria, is not lower than 2*10<9> CFU / g, a preservation number of the lactobacillus salivarius is CGMCC No.11386, and the content of the lactobacillus salivarius with is not lower than 5*10<9> CFU / g. The compound preparation and the application have the advantages that obvious effects of inhibiting avian Escherichia coli, clostridium perfringens, salmonella and the like can be realized by the compound preparation, the compound preparation can be applied to raising the broilers, accordingly, the necrotic enteritis morbidity and mortality due to the clostridium perfringens can be obviously reduced, and growth of the broilers can be promoted.

Description

technical field [0001] The invention belongs to the technical field of microecology, and in particular relates to a compound preparation for preventing broiler necrotic enteritis and application thereof. Background technique [0002] Chicken necrotic enteritis is a common enterotoxic disease in the poultry industry. The disease is characterized by sudden onset and acute death, with a high fatality rate, causing huge economic losses to the chicken industry. There are many cases, most of which occur in chickens aged 2 to 8 weeks, especially broiler chickens, and the incidence rate is showing an increasing trend year by year in our country. The excessive proliferation of Clostridium perfringens in the intestinal tract is one of the important factors causing necrotic enteritis in poultry. [0003] The traditional prevention and treatment method is to add antibiotics to drinking water or feed, but excessive reliance on antibiotics will lead to enhanced drug resistance of bacteri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8962A61P31/04A61P1/00A23K20/00A23K50/75A61K35/747A61K35/742A61K36/605
CPCA61K35/747A61K35/742A61K36/605A61K36/8962A61K2300/00
Inventor 王娇于刚王欣志赵世伟张大伟赵志强王政
Owner QINGDAO GENYUAN BIOLOGICAL TECH GRP
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