Bioartificial liver based on human iPSCs-induced hepatocyte-like cells and multilayer porous bioreactor
A bioartificial and liver cell technology, applied in the field of bioartificial liver, can solve the problems of animal-derived virus infection, no therapeutic effect on ALF mice, low metabolic function, etc., to promote 3D aggregate growth, promote liquid exchange efficiency, and carry cells The effect of a large amount
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[0026] In order to enable those skilled in the art to better understand the technical solution of the present invention, the embodiments of the present invention will be further described in detail below in conjunction with the accompanying drawings.
[0027] Such as Figure 1~4 As shown, a bioartificial liver based on human iPSCs induced hepatocytes and multilayer porous bioreactor, including plasma separation / reinfusion circulation part and cell reactor / plasma component exchange double circulation part, the two parts pass through the circulation pipeline Connected to each other, the connecting pipeline is made of medical grade polyvinyl chloride;
[0028] The plasma separation / reinfusion cycle part includes a blood input tube 1, a blood input peristaltic pump 2, a heparin pump 3, a plasma separation column 4, a first pressure monitor 5 and a heater 6, which are sequentially connected through a connecting pipeline, and the blood input tube 1 An exhaust pipe spring clip 7 is ...
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