Nanometer antibody for resisting salmonella enteritidis and application thereof
A Salmonella Enteritidis, nanobody technology, applied in application, antibacterial immunoglobulin, biochemical equipment and methods, etc., to achieve the effect of rapid detection method, strong specificity and strong stability
Active Publication Date: 2018-11-23
NORTHWEST A & F UNIV
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Problems solved by technology
However, so far, nanobodies against Salmonella Enteritidis have not been reported
Method used
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Embodiment 1
[0110] Example 1. Detection of Salmonella enteritidis in milk
[0111] Buy milk from the market, use the plate counting method to identify the absence of Salmonella Enteritidis, add 1 CFU / mL of Salmonella, after 24 hours of enrichment, use the Nanobody NB13 of the present invention to detect using the established double-antibody sandwich enzyme-linked immunoassay . It can be seen from Table 1 that the established enzyme-linked immunoassay method can detect Salmonella Enteritidis in milk samples after 24 hours of enrichment.
[0112] Table 1 Enzyme-linked immunoassay method for detection of Salmonella enteritidis in enriched milk
[0113]
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The invention relates to a nanometer antibody for resisting salmonella enteritidis and application thereof. The antibody has the function of being combined with the salmonella enteritidis. The invention also discloses the nanometer antibody and a gene sequence for coding the nanometer antibody, an expression carrier and a host cell of the nanometer antibody, and a method for producing the nanometer antibody. The nanometer antibody for resisting the salmonella enteritidis has the advantages that the size is small; the expression efficiency is high; the dissolution performance is good; the stability is high, and the like. The invention also provides the application of the nanometer antibody for resisting the salmonella enteritidis.
Description
technical field [0001] The invention relates to the fields of molecular biology, phage display technology and proteomics, in particular to a nanobody against Salmonella enteritidis and its application. Background technique [0002] Salmonella is a Gram-negative short bacillus. There are more than one thousand species found so far. It can cause animal diseases such as livestock, rodents and poultry. Once it contaminates human food, it will cause human food poisoning. Salmonella mainly includes A. paratyphi, B. paratyphi, Bacillus typhimurium, C. paratyphi, Bacillus choleraesuis, Bacillus typhi and Bacillus enteritidis. Among them, Salmonella enteritidis is highly invasive, mainly contaminating meat, eggs, poultry and aquatic products, and can cause human enteritis and food poisoning. According to statistics, in developed countries such as Japan and the United States, nearly 80% of the food poisoning incidents caused by Salmonella enteritidis have caused serious impacts on hu...
Claims
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IPC IPC(8): C07K16/12C12N15/13C12P21/02G01N33/569
CPCC07K16/1235C07K2317/565C07K2317/567C07K2317/569C07K2317/94G01N33/56916
Inventor 王妍入何一欣王建龙张道宏
Owner NORTHWEST A & F UNIV
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