Composite enrichment medium of salmonella, shigella and staphylococcus aureus, preparation method and application thereof

Abstract

The invention discloses a composite enrichment medium of salmonella, shigella and staphylococcus aureus. The composite enrichment medium comprises the following components in parts by weight: 5-15 parts of tryptone, 2.5-7.5 parts of a yeast extract, 5-15 parts of sodium chloride, 1-3 parts of glucose, 1-3 parts of sodium citrate, 1-2.5 parts of magnesium sulfate, 1-2.5 parts of L-phenylalanine, 1-3 parts of mannitol, 1-2.5 parts of sodium pyruvate and 1000 parts of distilled water. In the invention, the three types of the bacteria are the main detection parts of the national food hygienic standard pathogenic bacteria and can form proliferation advantage during the culture process of the composite enrichment medium, and the bacterial enrichment speed is faster and relatively consistent; after bacterial enrichment is carried out for 4 hours, separation culture, a biochemical authentication experiment and PCR authentication are performed; and after bacterial enrichment is carried is carried out for 18-24h, bacteria in an active non-culture status can make an accurate detection report, thus achieving wide application range and large usage amount, effectively lowering cost, simplifyingdetection procedures, enhancing working efficiency and being fast and accurate.

Description

technical field [0001] The invention belongs to the field of food testing, and in particular relates to a compound enrichment medium for Salmonella, Shigella and Staphylococcus aureus at the same time, a preparation method and application thereof. Background technique [0002] my country is the largest producer and consumer of livestock products in the world. Although food safety has attracted much attention in recent years, quality problems of livestock products still exist. Pathogen contamination is currently a prominent problem in the quality and safety of livestock products in my country. Salmonella, Shigellae, and Staphylococcus aureus are the main pathogenic bacteria that cause food poisoning, and are important monitoring items in food safety risk prediction and hazard assessment. , Cooked meat products, egg products, dairy products and other food testing standards must be tested for pathogenic bacteria. For this reason, the establishment of the simultaneous detection...

AI Technical Summary

Problems solved by technology

The SEL selective nutrient broth developed by Hyochin Kim et al. can simultaneously enrich Salmonella, Escherichia coli, and Listeria monocytogenes. The inoculation amount of each bacteria is about 100 CFU / mL. 6 -10 8 CFU / mL; BSB enrichment medium developed by Xu Yiping can simultaneously multiply Salmonella, Escherichia coli and Staphylococcus aureus. The medium components are: peptone, beef extract, sodium chloride, disodium hydrogen phosphate, Potassium dihydrogen phosphate, distilled water, the inoculum amount is about 10CFU / mL, after 18 hours of enrichment culture, the bacterial concentration can reach 10 6 -10 7 CFU / mL, after enrichment can meet multiple PCR detection; the SSL selective enrichment medium developed by Yu Yigang et al. can simultaneously cultivate Salmonella, Listeria monocytogenes and Staphylococcus aureus, and the inoculum volume is about 100CFU / mL mL, after 24 hours of compound culture, the bacterial concentration can reach 10 7 -10 8 CFU / mL, using fluorescent PCR amplification, the amplification curves of the three target bacteria can be obtained at the same time; in addition, there are some general-purpose enrichment media such as BPW (buffered peptone water), NB (nutrient broth), UPB, etc. Simultaneous propagation of multiple bacteria, but these media are used for a variety of bacteria, there is no compound enrichment medium for Salmonella, Shigella and Staphylococcus aureus

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