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Fluorescent sensor for detecting mycotoxins and application method thereof

A mycotoxin and aptamer sensor technology, applied in the field of analytical chemistry, can solve the problems of complex and expensive experimental equipment, long-term detection and analysis of samples, and poor photostability of fluorescent dye groups, achieving low cost, good specificity, The effect of high sensitivity

Inactive Publication Date: 2017-03-22
BEIJING UNIV OF CHEM TECH
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Problems solved by technology

The above detection methods require a certain operation process such as complex detection sample pretreatment, and at the same time it takes a long time to detect and analyze samples, and there are problems such as the complicated and expensive experimental equipment used for detection.
However, the method of constructing fluorescent sensors based on nucleic acid aptamers is limited by the poor photostability of traditional fluorescent dye groups.

Method used

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  • Fluorescent sensor for detecting mycotoxins and application method thereof
  • Fluorescent sensor for detecting mycotoxins and application method thereof
  • Fluorescent sensor for detecting mycotoxins and application method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] A fluorescent aptasensor for detecting ochratoxin A:

[0027] (1) DNA hybridization makes the graphene quantum dots aggregate and the fluorescence signal is quenched: firstly, the powdered ochratoxin A aptamer DNA and the complementary short-chain probe DNA are dissolved by centrifugation in TE buffer, and then the hydrothermal method is used to Graphene quantum dots were synthesized, and ochratoxin A aptamer DNA and complementary short-chain probe DNA were respectively modified on the surface, and the two graphene quantum dot solutions were mixed to generate graphene quantum dots through DNA hybridization. Aggregation occurs and the fluorescence signal is quenched;

[0028] (2) Fluorescence detection of ochratoxin A: Add different concentrations of ochratoxin A into the quenching solution, incubate at room temperature for 1 h, set the excitation emission wavelength and incident emission slit of the fluorescence spectrophotometer, and take The incubated reaction soluti...

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Abstract

A fluorescent sensor for detecting mycotoxins and an application method thereof belong to the field of analytical chemistry. The surfaces of two groups of graphene quantum dots are modified respectively with aptamer DNA corresponding to mycotoxins or probe DNA complementary to the aptamer DNA, the graphene quantum dots are aggregated by DNA hybridization, exciton energy is transferred, and graphene quantum dot fluorescent signals are cancelled at last; mycotoxins are added then and engage in specific binding with the aptamer DNA, structural changes occur, the graphene quantum dot aggregation is disassembled and re-dispersed, and the system fluorescent strength is restored. The preparation method of the fluorescent sensor for detecting mycotoxins has a simple and feasible process, and the fluorescent sensor is green, low in cost, high in sensitivity and good in specificity and is successfully applied to standard added recovery of mycotoxins in actual wine samples.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and specifically relates to a method for detecting mycotoxins by using a nucleic acid aptamer structure conversion strategy to regulate the aggregation state of graphene quantum dots, thereby regulating the quenching and recovery of fluorescent signals. Background technique [0002] Mycotoxins (mycotoxins) mainly refer to the toxic metabolites produced by molds in the food they contaminate. They are anti-nutritional factors present in feed and raw materials, and are secondary metabolites of molds with strong toxins. They can enter human and animal body through feed or food, cause acute or chronic toxicity to human and animal, and damage the liver, kidney, nerve tissue, hematopoietic tissue and skin tissue of the body, etc. [0003] Ochratoxin A (Ochratoxin A), referred to as OTA, is a group of toxic metabolites produced by certain strains of aspergillus and penicillin, which have nephrotoxicit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 张瑛洧汪松
Owner BEIJING UNIV OF CHEM TECH
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