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Method for measuring content of mycotoxins in araliaceae plants by liquid chromatography-tandem mass spectrometry

A technology of mycotoxins and liquid chromatography, which is applied in the field of determination of mycotoxins in Araliaceae plants, can solve the problem that there is no simultaneous detection of multi-component residues, etc., and achieve the effects of good accuracy, strong specificity and high sensitivity

Inactive Publication Date: 2012-09-05
SHANGHAI INST FOR FOOD & DRUG CONTROL
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  • Abstract
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Problems solved by technology

In terms of food, my country has successively promulgated many statutory standards on mycotoxins (see GB2715-2005 Mycotoxin Limits in Food [S] and GB2761-2005 Mycotoxin Limits in Food [S]), but in the field of pharmaceuticals, except for the Chinese Pharmacopoeia Except for the detection method of aflatoxin (see Pharmacopoeia of the People's Republic of China 2010 Edition [S]), no other standards have been issued
However, the existing food standards are all for the detection of a single mycotoxin, and there is no standard for simultaneous detection of multi-component residues

Method used

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  • Method for measuring content of mycotoxins in araliaceae plants by liquid chromatography-tandem mass spectrometry
  • Method for measuring content of mycotoxins in araliaceae plants by liquid chromatography-tandem mass spectrometry
  • Method for measuring content of mycotoxins in araliaceae plants by liquid chromatography-tandem mass spectrometry

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Experimental program
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Effect test

Embodiment 1

[0030] 1. Materials and methods

[0031] 1.1 Main instruments and reagents

[0032] API 4000 tandem quadrupole mass spectrometer (Applied Biosystems, USA), ACQUITY Ultra Performance LC liquid chromatograph (Waters, USA), MIKRO 200R centrifuge (Hettich, Germany), ultrapure water machine (Millipore, USA).

[0033] Aflatoxin, deoxynivalenol, zearalenone, and T-2 toxin standard (SUPELCO, USA); acetonitrile, formic acid, and ammonium formate were all chromatographically pure. Mycosep 226 multifunctional purification column (Romer company). Ginseng was purchased from Shanghai Huayu Medicinal Materials Co., Ltd., place of origin: Jilin, batch number: 20040404.

[0034] 1.2 Experimental method

[0035] 1.2.1 Chromatographic conditions

[0036] ACQUITY UPLC BEH C18 (1.7μm, 2.1×100mm); 100% methanol as mobile phase A, 0.01% formic acid as mobile phase B, flow rate 0.3ml / min; gradient elution according to the following table:

[0037] Table 1. Mobile phase gradient

[0038]

[0...

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Abstract

The invention discloses a method for measuring the content of mycotoxins in araliaceae plants by liquid chromatography-tandem mass spectrometry, comprising the following steps of: (1) preprocessing an araliaceae plant sample to be measured by a solid-phase extraction column; and (2) measuring the content of one or more mycotoxins in the preprocessed araliaceae plant sample through the liquid chromatography-tandem mass spectrometry. The measurement method is high in sensitivity, good in specificity and good in accuracy, and applied to measurement of multiple component residuals of the mycotoxins in the araliaceae plants.

Description

technical field [0001] The invention relates to a method for determining the content of mycotoxins in Araliaceae plants, in particular to a method for determining the content of mycotoxins in Araliaceae plants by liquid chromatography-tandem mass spectrometry. Background technique [0002] Mycotoxin, also known as mycotoxin, is a secondary metabolite produced by fungi, which generally has the characteristics of strong toxicity and high pollution frequency. Due to the parasitism of fungi and the production of mycotoxins, the yield of crops is seriously affected, the quality of agricultural products and feed is reduced, and huge economic losses are caused. Ingestion of agricultural and animal products contaminated by mycotoxins by humans or animals, or contact with mycotoxins through inhalation and skin can cause a variety of poisoning symptoms. Such as hallucinations, vomiting, hemorrhage, dermatitis, damage to the central nervous system, and even death. The results of anim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 郑荣季申王少敏简龙海王柯毛丹许勇毛秀红胡青夏晶李丽敏张道广
Owner SHANGHAI INST FOR FOOD & DRUG CONTROL
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