Composite micro-ecological feed additive and preparation method and premix thereof

A technology of feed additive and compound microecology, which is applied in the field of compound microecological feed additive and its premix, to achieve the effect of preventing and treating diarrhea, reducing content and strong antibacterial effect

Active Publication Date: 2012-01-25
BEIJING DABEINONG TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many feed microecological preparations on the market, and their effects are mainly focused on improving animal immunity and increasing animal production capa

Method used

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  • Composite micro-ecological feed additive and preparation method and premix thereof
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  • Composite micro-ecological feed additive and preparation method and premix thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the preparation of compound probiotics feed additive

[0023] A. Preparation of Lactobacillus acidophilus bacteria powder: Lactobacillus acidophilus CGMCC NO.5093 was inoculated in MRS medium, cultured statically at 37°C for 12 hours, and the bacterial concentration was adjusted to 2×10 9 CFU / ml, according to the ratio (volume ratio) of bacterial solution: protective agent 1:10, add protective agent (the formula of protective agent is skim milk 3%, trehalose 2%, manganese sulfate 0.5%, sucrose 0.2%, others are water ), after pre-freezing at -80°C for 4 hours, adopt the method of vacuum freeze-drying, and make the viable count of 2×10 8 CFU / g Lactobacillus acidophilus powder.

[0024]B. Preparation of Bacillus pumilus powder: Bacillus pumilus CGMCC NO.4756 was inoculated in LB medium, cultured statically at 37°C for 12 hours, and the bacterial concentration was adjusted to 5×10 11 CFU / ml, according to the ratio (weight ratio) of bacteria solution:carrier ...

Embodiment 2

[0027] Embodiment 2: the preparation of compound probiotics feed additive

[0028] A. Preparation of Lactobacillus acidophilus bacteria powder: Lactobacillus acidophilus CGMCC NO.5093 was inoculated in MRS medium, cultured statically at 37°C for 12 hours, and the bacterial concentration was adjusted to 2×10 9 CFU / ml, according to the ratio (volume ratio) of bacterial solution: protective agent 1:10, add protective agent (the formula of protective agent is skim milk 3%, trehalose 2%, manganese sulfate 0.5%, sucrose 0.2%, others are water ), after pre-freezing at -80°C for 4 hours, use the method of vacuum freeze-drying to make the number of viable bacteria 5×10 at the freezing temperature of -50°C and vacuum degree of 30Pa 8 CFU / g Lactobacillus acidophilus powder.

[0029] B. Preparation of Bacillus pumilus powder: Bacillus pumilus CGMCC NO.4756 was inoculated in LB medium, cultured statically at 37°C for 12 hours, and the bacterial concentration was adjusted to 5×10 11 CFU / m...

Embodiment 3

[0032] Embodiment 3: the preparation of compound probiotics feed additive

[0033] A. Preparation of Lactobacillus acidophilus bacteria powder: Lactobacillus acidophilus CGMCC NO.5093 was inoculated in MRS medium, cultured statically at 37°C for 12 hours, and the bacterial concentration was adjusted to 2×10 9 CFU / ml, according to the ratio (volume ratio) of bacterial solution: protective agent 1:10, add protective agent (the formula of protective agent is skim milk 3%, trehalose 2%, manganese sulfate 0.5%, sucrose 0.2%, others are water ), after pre-freezing at -80°C for 4 hours, using vacuum freeze-drying method, the number of viable bacteria was 6×10 at freezing temperature -50°C and vacuum degree of 30Pa. 8 CFU / g Lactobacillus acidophilus powder.

[0034] B. Preparation of Bacillus pumilus powder: Bacillus pumilus CGMCC NO.4756 was inoculated in LB medium, cultured statically at 37°C for 12 hours, and the bacterial concentration was adjusted to 5×10 11 CFU / ml, according t...

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Abstract

The invention discloses a composite micro-ecological feed additive and a premix thereof, and belongs to the technical field of feed science and feed additive. The feed additive contains multiple probiotics, and has the functions of enhancing the immunity of livestock and poultry and improving the production performance of the livestock and poultry; and meanwhile, the feed additive can be used as a feed mildew-proof agent, has good adhesion identifying and removing capacity on feed mycotoxins, can effectively inhibit the growth of mycete in the feed and adsorb and degrade the mycotoxins, is a novel, safe, reliable and pollution-free pure fermented multifunctional micro-ecological preparation product, and has good application prospect.

Description

technical field [0001] The invention relates to a compound micro-ecological feed additive and a premix thereof, belonging to the technical field of feed science and feed additives. Background technique [0002] With the rapid development of the aquaculture industry, people's research on feed additives has involved various aspects. Among them, the safety of drug feed additives such as antibiotics and the contamination of feed by mold and mycotoxins have become important issues in the current feed industry and animal husbandry production. Two hot spots of research. [0003] According to the estimates of the United Nations Food Organization, about 5% to 7% of agricultural products such as grain and feed are damaged by molds in the world every year, which has caused huge economic losses. The feed contaminated by mold will decompose its nutrients, reduce its palatability, and produce a variety of mycotoxins. Animals will experience acute or chronic poisoning symptoms after eatin...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K10/16A23K10/18A23K20/163A23K20/20
Inventor 刘滢彭子欣王安如谢飞胡婷刘婷
Owner BEIJING DABEINONG TECH GRP CO LTD
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