LAMP (Loop-Mediated Isothermal Amplification) detection kit and detection method for giant salamander iridovirus

A giant salamander iridescent virus and detection kit technology is applied in the giant salamander iridescent virus LAMP detection kit, and the giant salamander iridescent virus LAMP detection kit is used to detect the giant salamander iridescent virus field, which can solve the problems of economic loss of the giant salamander breeding industry, restricting the development of healthy breeding and the like , to achieve the effect of low detection cost, high sensitivity and good specificity
CN103014181AActive Publication Date: 2013-04-03YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Publication Date
2013-04-03

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Abstract

The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) detection kit and a detection method for giant salamander iridovirus. The LAMP detection kit for the andrias advidianus iridescent virus comprises the following ingredients: 10*Thermol Reaction buffer, BstDNA polymerase, dNTPs, outer primers F3 and B3, inner primers FIP and BIP, BEtaine, MGc12 and 1000*SYBRGreenI. The LAMP detection kit and the LAMP detection method have the characteristics of simplicity, convenience, high specificity and sensitivity, the GSIV (Giant Salamander Iridovirus) in a sample can be detected accurately within 2h by a water bath kettle or a metal bath kettle, the sick fish tissues infected by GSIV can be detected, and the cells infected by the GSIV can be detected, so that the detection kit and the detection method are very suitable for field rapid detection of GSIV.
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Description

technical field

[0001] The invention belongs to the technical field of fish virus detection, in particular to a giant salamander iridescent virus LAMP detection kit, and also relates to a method for detecting giant salamander iridescent virus LAMP detection kit. Background technique

[0002] The giant salamander (Andrias davidianus), commonly known as the giant salamander, belongs to Amphibians, Cauracea, Cryptobranchidae, and the genus Giant salamander. It was listed as a second-class national protected animal in 1988. The 14th Conference of the Parties was listed as a Class I endangered species. Giant salamander not only has certain medicinal and scientific value, but also can be used as ornamental animals and edible delicacies. Since the 1980s, artificial breeding and breeding of giant salamanders have been carried out successively in various parts of my country, and it has become one of the excellent species of high-efficiency breeding in aquaculture. However, with the...

Claims

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