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Preparation method of andrias oligopeptide

A technology of oligopeptides and giant salamanders, which is applied in the field of preparation of giant salamander oligopeptides, can solve problems such as unclear functions, and achieve the effects of wide application, less loss of nutrients, and high safety

Inactive Publication Date: 2013-10-23
四川龙王洞生态农业开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, in recent years, domestic research on various peptide biotechnology preparation methods has gradually deepened and developed. Among them, there are also studies on the hydrolysis of giant salamander protease. The preparation of the few giant salamander polypeptides is limited to the optimization of enzymatic hydrolysis conditions. Functions are limited to methods such as relieving physical fatigue; Chinese patent publication "CN102676622A" discloses a patent application for "Giant salamander nanopeptide and giant salamander wine", from which it can be seen that the method is only to obtain giant salamander "by simple enzymolysis of nano giant salamander powder" "Nanopeptides" and more free amino acids, the resulting products are questionable, and their functions are not very clear

Method used

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  • Preparation method of andrias oligopeptide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1 preprocessing

[0029] After the artificial breeding of fresh giant salamanders is slaughtered, the fresh giant salamander meat is freeze-dried and crushed to 60 mesh at 0°C to obtain giant salamander meat powder, which is put into 3 times its mass of edible oil extraction agent n-hexane The fat is removed by leaching, and the solvent is evaporated to obtain defatted giant salamander powder.

[0030] 2 enzymatic hydrolysis

[0031] The animal hydrolytic protease and neutral protease are mixed according to the mass ratio of 3:1 to form a compound enzyme. In the case of a material-to-water ratio of 1:3, 100,000 activity units of compound enzyme are added, and the amount added is 0.2% of the raw material; the compound enzyme powder is made of Hot water pretreatment, the amount of hot water is 20 times the mass of the compound enzyme powder, the temperature is 35°C, and the pretreatment time is 10 minutes; after pretreatment, stir well and filter, add to the enzymatic ...

Embodiment 2

[0041] 1 preprocessing

[0042] After slaughtering the artificially bred fresh giant salamander, take the fresh giant salamander meat, freeze-dry it, and crush it to 80 mesh at -2°C to obtain giant salamander meat powder, which is put into 2.5 times its mass of edible oil extraction agent Zhengji The fat is removed by leaching with alkane, and the solvent is evaporated to obtain defatted giant salamander powder.

[0043] 2 enzymatic hydrolysis

[0044] The animal hydrolytic protease and neutral protease are mixed according to the mass ratio of 3:1.2 to form a compound enzyme. In the case of 1:3.5 material-water ratio, 100,000 activity units of compound enzyme are added, and the amount added is 0.4% of the raw material; the compound enzyme powder adopts Hot water pretreatment, the amount of hot water is 30 times the mass of the compound enzyme powder, the temperature is 40 ° C, the pretreatment time is 12 minutes; after pretreatment, stir well and filter, add to the enzymati...

Embodiment 3

[0054] 1 preprocessing

[0055] After slaughtering the artificially bred fresh giant salamander, take the fresh giant salamander meat, freeze-dry it, and crush it to 80 mesh at -4°C to obtain giant salamander meat powder, which is put into edible oil extractant Zhengji with twice its mass. The fat is removed by leaching with alkane, and the solvent is evaporated to obtain defatted giant salamander powder.

[0056] 2 enzymatic hydrolysis

[0057] The animal hydrolytic protease and neutral protease are mixed according to the mass ratio of 3:1.4 to form a compound enzyme. In the case of a material-to-water ratio of 1:3, 100,000 activity units of the compound enzyme are added, and the amount added is 0.5% of the raw material; the compound enzyme powder is made of Hot water pretreatment, the amount of hot water is 25 times the mass of the compound enzyme powder, the temperature is 45°C, and the pretreatment time is 15 minutes; after pretreatment, stir and filter, add to the enzy...

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Abstract

The invention discloses a preparation method of andrias oligopeptide. The preparation method takes human-bred andrias as the raw material and obtains andrias oligopeptide through enzymolyzing andrias protein by animal protease and neutral protease. The preparation method comprises steps of pre-treating, enzymolyzing, purifying, inactivating enzyme, debittering, decoloring sterilizing, condensing and freeze drying. The preparation method has the advantages that: the hydrolysis conditions are mild, specific polypeptide can be generated by positioning hydrolysis, and the hydrolysis process is easy to control. The product has the advantages of easiness in digestion and absorption, high safety, little nutrition loss during the production and environmental friendliness. The product can be applied to medicine raw material, drug, and functional food. In the same time, the preparation method has the advantages of advanced technology, safety, environmental-friendliness, vast application areas, low cost, high addition value, and suitability for industrial production.

Description

technical field [0001] The invention relates to a preparation method of giant salamander oligopeptide, which belongs to the field of bioactive peptides. Background technique [0002] The giant salamander, commonly known as the giant salamander, belongs to the family Cryptobranchidae of the order Amphibians and is a relatively primitive species among the existing amphibians. It is a living fossil with a long history of 350 million years in my country. It is comparable to giant pandas. It is a traditional medicinal animal in my country. There are also a lot of folk remedies, and the results of modern nutritional composition analysis show that it is rich in nutrition, contains 18 kinds of amino acids, and has a comprehensive composition of ingredients. The giant salamander is a magical and ancient precious animal. The long-term historical practice has proved that it has many specific physiological functions for the human body, such as intelligence development, anti-aging, impro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K4/12C07K1/36C07K1/34
Inventor 阳爱生刘绍邱平
Owner 四川龙王洞生态农业开发有限公司
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