Collagen peptide composition for enhancing immunostimulation osteoblast synthesis and preparation method thereof

Abstract

The invention belongs to the technical field of biological medicines, and particularly discloses a collagen peptide composition for enhancing immunostimulation osteoblast synthesis and a preparation method thereof, the collagen peptide composition comprises the following components by mass: 0.05-0.2% of taurine, 0.1-1% of vitamin C, 5-20% of type II collagen peptide, 5-20% of type III collagen peptide, and the balance of bovine bone collagen peptide. On the basis of the bovine bone collagen peptide, under the action of the taurine, the vitamin C, the II type collagen peptide, the III type collagen peptide and the red date extract, immunity can be effectively improved, osteoblasts are stimulated to synthesize the collagen peptide, and therefore the bone mineral density is effectively increased, and the osteoporosis is reduced. Wherein the red date extract and the bovine bone collagen peptide have a remarkable synergistic effect.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedicine, and in particular to a composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis and a preparation method thereof. Background Art

[0002] Collagen peptides are functional peptides with a triple helix structure and a short sequence of 2 to 20 amino acid residues, which are made from collagen after enzymatic and thermal hydrolysis. Due to its good compatibility and degradability, it has gradually become a research hotspot in the fields of medicine, biomaterials, and beauty and skin care in recent years. A large number of studies have confirmed that collagen peptides have many functions such as delaying skin aging, preventing and treating osteoporosis, anti-oxidation, alleviating liver damage, promoting wound healing, enhancing immunity, and improving learning and memory, and have great application potential.

[0003] Collagen peptides have a significant effect in enhancing immunity. Collagen peptides also show potential in promoting osteoblast synthesis. Bovine collagen peptide composition is one of the representative products, which usually contains bovine collagen peptides, glucosamine chondroitin and plant extracts.

[0004] In view of this, this application is filed. Summary of the invention

[0005] The present invention provides a composition of collagen peptides synthesized by enhancing immunity and stimulating osteoblasts and a preparation method thereof. The composition of the present invention can effectively increase immunity, stimulate the collagen peptides synthesized by osteoblasts, and thus improve bone density.

[0006] The present invention solves the technical problem by adopting the following technical solutions: A composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis comprises the following components in percentage by mass: 0.05-0.2% taurine, 0.1%-1% vitamin C, 5%-20% type II collagen peptides, 5%-20% type III collagen peptides, and the remainder bovine bone collagen peptides.

[0007] The present invention creatively combines the above-mentioned raw materials, based on bovine bone collagen peptide, and under the action of taurine, vitamin C, type II collagen peptide, type III collagen peptide, and red date extract, it can effectively increase immunity, stimulate osteoblasts to synthesize collagen peptides, and thus effectively improve bone density, wherein the red date extract and the bovine bone collagen peptide have a significant synergistic effect.

[0008] As a preferred embodiment of the present invention, the following components are included in mass percentage: 0.01-0.15% taurine, 0.4%-0.6% vitamin C, 10%-15% type II collagen peptide, 10%-15% type III collagen peptide, and the remainder bovine bone collagen peptide.

[0009] As a preferred embodiment of the present invention, the preparation method of the bovine collagen peptide is: (1) Selecting cattle shank bones, removing tendons and meat, washing, and crushing to obtain cattle shank bone powder; (2) adding the cattle shank bone powder into an acetic acid solution for soaking and decalcification to obtain decalcified cattle shank bone powder; (3) Add decalcified bovine bone powder, papain, neutral protease, and β-glucanase into water, perform enzymatic hydrolysis at 45-55°C for 2-6 hours, filter, and dry to obtain bovine bone collagen peptide.

[0010] The bovine collagen peptide synthesized by the present invention has a molecular weight of 500-3000DA. The bovine collagen peptide can promote the regeneration of skin collagen, increase skin elasticity, reduce the formation of wrinkles, make the skin more firm, smooth and soft, improve the skin's water retention capacity, help improve skin dryness, sagging and other problems, effectively increase bone density, and prevent osteoporosis.

[0011] As a preferred embodiment of the present invention, the concentration of the acetic acid solution is 0.05-0.2 mol / L.

[0012] As a preferred embodiment of the present invention, the mass ratio of the decalcified cattle bone meal, papain, neutral protease, β-glucanase and water is 1: (0.005-0.02): (0.005-0.02): (0.005-0.02): (4-10).

[0013] As a preferred embodiment of the present invention, the following components are also included in mass percentage: 20-30% red date extract; preferably 24-28% red date extract.

[0014] As a preferred embodiment of the present invention, the preparation method of the red date extract is: (1) crushing red dates to obtain red date powder; (2) adding red date powder, cellulase, alkaline protease and β-glucanase into water, performing ultrasonic enzymolysis at 45-55°C for 2-6 hours to obtain an enzymolysis solution; (3) The pH value of the enzymatic hydrolyzate was adjusted to 6.2-6.8, and the macroporous adsorption resin was applied at a rate of 0.5-1.5 BV / h and an elution rate of 1-2 BV / h. First, 1-2 BV was washed with deionized water, and then 1-3 BV was washed with 40-60 wt% ethanol solution. The ethanol washing liquid was collected and dried to obtain the red date extract.

[0015] The red date extract prepared by the present invention can promote the proliferation and differentiation of osteoblasts, inhibit the survival rate of osteoclasts, and induce their apoptosis, thereby slowing down the bone absorption process, protecting bone health, improving osteoporosis, and increasing bone density.

[0016] As a preferred embodiment of the present invention, the mass ratio of the red date powder, cellulase, alkaline protease, β-glucanase and water is 1: (0.005~0.02): (0.005~0.02): (0.005~0.02): (4~10).

[0017] As a preferred embodiment of the present invention, the power of the ultrasound is 300~600W.

[0018] As a preferred embodiment of the present invention, the macroporous adsorption resin is D101 macroporous adsorption resin.

[0019] The present invention also provides a method for preparing a composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis, comprising the following steps: uniformly mixing taurine, vitamin C, type II collagen peptides, type III collagen peptides, red date extracts, and bovine bone collagen peptides to obtain the composition.

[0020] Beneficial effects of the present invention: The present invention is based on bovine collagen peptide, and under the action of taurine, vitamin C, type II collagen peptide, type III collagen peptide, and red date extract, it can effectively increase immunity, stimulate osteoblasts to synthesize collagen peptides, and thus effectively improve bone density, wherein the red date extract and the bovine collagen peptide have a significant synergistic effect. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] In the present invention, the technical features described in an open manner include closed technical solutions composed of the listed features, and also include open technical solutions containing the listed features.

[0023] In the present invention, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are deemed to be continuous and include the minimum and maximum values ​​of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges included therein.

[0024] In the present invention, there is no particular limitation on the specific dispersion and stirring treatment methods.

[0025] The reagents or instruments used in the present invention without indicating the manufacturer are all conventional products that can be obtained commercially. The raw materials used in the comparative examples and the raw materials used in the parallel experiments of the embodiments are the same commercially available products unless otherwise specified.

[0026] The type III collagen peptides of the present invention and comparative examples were purchased from Shengminuo and Cuiermei ® Type III collagen peptides.

[0027] Type II collagen peptide was purchased from SEMFLEX-II ® Non-denatured type II collagen.

[0028] Example 1 A composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis comprises the following components in percentage by mass: 0.0125% taurine, 0.5% vitamin C, 12% type II collagen peptide, 12% type III collagen peptide, 25% red date extract, and the remainder bovine bone collagen peptide.

[0029] The preparation method of the bovine collagen peptide is as follows: (1) Selecting cattle shank bones, removing tendons and meat, washing, and crushing to 40 meshes to obtain cattle shank bone powder; (2) adding the cattle shank bone powder into a 0.1 mol / L acetic acid solution and soaking for 12 hours to decalcify, thereby obtaining decalcified cattle shank bone powder; (3) Adding decalcified bovine shank bone powder, papain, neutral protease, and β-glucanase into water, performing enzymatic hydrolysis at 50° C. for 5 hours, filtering, and drying to obtain bovine bone collagen peptide; the mass ratio of the decalcified bovine shank bone powder, papain, neutral protease, β-glucanase, and water is 1:0.01:0.01:0.01:6.

[0030] The preparation method of the red date extract is as follows: (1) Wash the red dates, remove the pits and grind them into 100 mesh to obtain red date powder; (2) Add red date powder, cellulase, alkaline protease, and β-glucanase into water, perform ultrasonic enzymolysis at 50°C for 5 h, and obtain an enzymolysis solution; the ultrasonic power is 500 W; (3) The pH value of the enzymatic hydrolyzate is adjusted to 6.6, and the macroporous adsorption resin is applied at a rate of 1 BV / h and an elution rate of 1.5 BV / h. First, 1.5 BV is washed with deionized water, and then 2 BV is washed with a 50 wt% ethanol solution. The ethanol washing liquid is collected and dried to obtain a red date extract. The mass ratio of the red date powder, cellulase, alkaline protease, β-glucanase and water is 1:0.01:0.01:0.01:6.

[0031] The preparation method of the composition of collagen peptides for enhancing immunity and stimulating osteoblast synthesis comprises the following steps: taurine, vitamin C, type II collagen peptide, type III collagen peptide, red date extract and bovine bone collagen peptide are uniformly mixed to obtain the composition.

[0032] Example 2 A composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis comprises the following components in percentage by mass: 0.01% taurine, 0.4% vitamin C, 10% type II collagen peptides, 15% type III collagen peptides, 24% red date extract, and the remainder bovine bone collagen peptides.

[0033] The preparation method of the bovine collagen peptide is as follows: (1) Selecting cattle shank bones, removing tendons and meat, washing, and crushing to 40 meshes to obtain cattle shank bone powder; (2) adding the cattle shank bone powder into a 0.1 mol / L acetic acid solution and soaking for 12 hours to decalcify, thereby obtaining decalcified cattle shank bone powder; (3) Adding decalcified bovine shank bone powder, papain, neutral protease, and β-glucanase into water, performing enzymatic hydrolysis at 50° C. for 5 hours, filtering, and drying to obtain bovine bone collagen peptide; the mass ratio of the decalcified bovine shank bone powder, papain, neutral protease, β-glucanase, and water is 1:0.01:0.01:0.01:6.

[0034] The preparation method of the red date extract is as follows: (1) Wash the red dates, remove the pits and grind them into 100 mesh to obtain red date powder; (2) Add red date powder, cellulase, alkaline protease, and β-glucanase into water, perform ultrasonic enzymolysis at 50°C for 5 h, and obtain an enzymolysis solution; the ultrasonic power is 500 W; (3) The pH value of the enzymatic hydrolyzate is adjusted to 6.6, and the macroporous adsorption resin is applied at a rate of 1 BV / h and an elution rate of 1.5 BV / h. First, 1.5 BV is washed with deionized water, and then 2 BV is washed with a 50 wt% ethanol solution. The ethanol washing liquid is collected and dried to obtain a red date extract. The mass ratio of the red date powder, cellulase, alkaline protease, β-glucanase and water is 1:0.01:0.01:0.01:6.

[0035] The preparation method of the composition of collagen peptides for enhancing immunity and stimulating osteoblast synthesis comprises the following steps: taurine, vitamin C, type II collagen peptide, type III collagen peptide, red date extract and bovine bone collagen peptide are uniformly mixed to obtain the composition.

[0036] Example 3 A composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis comprises the following components in percentage by mass: 0.15% taurine, 0.6% vitamin C, 15% type II collagen peptide, 10% type III collagen peptide, 28% red date extract, and the remainder bovine bone collagen peptide.

[0037] The preparation method of the bovine collagen peptide is as follows: (1) Selecting cattle shank bones, removing tendons and meat, washing, and crushing to 40 meshes to obtain cattle shank bone powder; (2) adding the cattle shank bone powder into a 0.1 mol / L acetic acid solution and soaking for 12 hours to decalcify, thereby obtaining decalcified cattle shank bone powder; (3) Adding decalcified bovine shank bone powder, papain, neutral protease, and β-glucanase into water, performing enzymatic hydrolysis at 50° C. for 5 hours, filtering, and drying to obtain bovine bone collagen peptide; the mass ratio of the decalcified bovine shank bone powder, papain, neutral protease, β-glucanase, and water is 1:0.01:0.01:0.01:6.

[0038] The preparation method of the red date extract is as follows: (1) Wash the red dates, remove the pits and grind them into 100 mesh to obtain red date powder; (2) Add red date powder, cellulase, alkaline protease, and β-glucanase into water, perform ultrasonic enzymolysis at 50°C for 5 h, and obtain an enzymolysis solution; the ultrasonic power is 500 W; (3) The pH value of the enzymatic hydrolyzate is adjusted to 6.6, and the macroporous adsorption resin is applied at a rate of 1 BV / h and an elution rate of 1.5 BV / h. First, 1.5 BV is washed with deionized water, and then 2 BV is washed with a 50 wt% ethanol solution. The ethanol washing liquid is collected and dried to obtain a red date extract. The mass ratio of the red date powder, cellulase, alkaline protease, β-glucanase and water is 1:0.01:0.01:0.01:6.

[0039] The preparation method of the composition of collagen peptides for enhancing immunity and stimulating osteoblast synthesis comprises the following steps: taurine, vitamin C, type II collagen peptide, type III collagen peptide, red date extract and bovine bone collagen peptide are uniformly mixed to obtain the composition.

[0040] Example 4 A composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis comprises the following components in percentage by mass: 0.05% taurine, 0.1% vitamin C, 5% type II collagen peptide, 20% type III collagen peptide, 20% red date extract, and the remainder bovine bone collagen peptide.

[0041] The preparation method of the bovine collagen peptide is as follows: (1) Selecting cattle shank bones, removing tendons and meat, washing, and crushing to 40 meshes to obtain cattle shank bone powder; (2) adding the cattle shank bone powder into a 0.1 mol / L acetic acid solution and soaking for 12 hours to decalcify, thereby obtaining decalcified cattle shank bone powder; (3) Adding decalcified bovine shank bone powder, papain, neutral protease, and β-glucanase into water, performing enzymatic hydrolysis at 50° C. for 5 hours, filtering, and drying to obtain bovine bone collagen peptide; the mass ratio of the decalcified bovine shank bone powder, papain, neutral protease, β-glucanase, and water is 1:0.01:0.01:0.01:6.

[0042] The preparation method of the red date extract is as follows: (1) Wash the red dates, remove the pits and grind them into 100 mesh to obtain red date powder; (2) Add red date powder, cellulase, alkaline protease, and β-glucanase into water, perform ultrasonic enzymolysis at 50°C for 5 h, and obtain an enzymolysis solution; the ultrasonic power is 500 W; (3) The pH value of the enzymatic hydrolyzate is adjusted to 6.6, and the macroporous adsorption resin is applied at a rate of 1 BV / h and an elution rate of 1.5 BV / h. First, 1.5 BV is washed with deionized water, and then 2 BV is washed with a 50 wt% ethanol solution. The ethanol washing liquid is collected and dried to obtain a red date extract. The mass ratio of the red date powder, cellulase, alkaline protease, β-glucanase and water is 1:0.01:0.01:0.01:6.

[0043] The preparation method of the composition of collagen peptides for enhancing immunity and stimulating osteoblast synthesis comprises the following steps: taurine, vitamin C, type II collagen peptide, type III collagen peptide, red date extract and bovine bone collagen peptide are uniformly mixed to obtain the composition.

[0044] Example 5 A composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis comprises the following components in percentage by mass: 0.2% taurine, 1% vitamin C, 20% type II collagen peptide, 5% type III collagen peptide, 30% red date extract, and the remainder bovine bone collagen peptide.

[0045] The preparation method of the bovine collagen peptide is as follows: (1) Selecting cattle shank bones, removing tendons and meat, washing, and crushing to 40 meshes to obtain cattle shank bone powder; (2) adding the cattle shank bone powder into a 0.1 mol / L acetic acid solution and soaking for 12 hours to decalcify, thereby obtaining decalcified cattle shank bone powder; (3) Adding decalcified bovine shank bone powder, papain, neutral protease, and β-glucanase into water, performing enzymatic hydrolysis at 50° C. for 5 hours, filtering, and drying to obtain bovine bone collagen peptide; the mass ratio of the decalcified bovine shank bone powder, papain, neutral protease, β-glucanase, and water is 1:0.01:0.01:0.01:6.

[0046] The preparation method of the red date extract is as follows: (1) Wash the red dates, remove the pits and grind them into 100 mesh to obtain red date powder; (2) Add red date powder, cellulase, alkaline protease, and β-glucanase into water, perform ultrasonic enzymolysis at 50°C for 5 h, and obtain an enzymolysis solution; the ultrasonic power is 500 W; (3) The pH value of the enzymatic hydrolyzate is adjusted to 6.6, and the macroporous adsorption resin is applied at a rate of 1 BV / h and an elution rate of 1.5 BV / h. First, 1.5 BV is washed with deionized water, and then 2 BV is washed with a 50 wt% ethanol solution. The ethanol washing liquid is collected and dried to obtain a red date extract. The mass ratio of the red date powder, cellulase, alkaline protease, β-glucanase and water is 1:0.01:0.01:0.01:6.

[0047] The preparation method of the composition of collagen peptides for enhancing immunity and stimulating osteoblast synthesis comprises the following steps: taurine, vitamin C, type II collagen peptide, type III collagen peptide, red date extract and bovine bone collagen peptide are uniformly mixed to obtain the composition.

[0048] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that Comparative Example 1 does not contain red date extract (replaced by an equal amount of bovine collagen peptide), and the rest is the same.

[0049] A composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis comprises the following components in percentage by mass: 0.0125% taurine, 0.5% vitamin C, 12% type II collagen peptides, 12% type III collagen peptides, and the remainder bovine bone collagen peptides.

[0050] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that Comparative Example 2 does not contain red date extract (replaced by an equal amount of bovine collagen peptide), and the rest is the same.

[0051] A composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis comprises the following components in percentage by mass: 0.0125% taurine, 0.5% vitamin C, 12% type II collagen peptides, 12% type III collagen peptides, and the remainder red date extract.

[0052] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that Comparative Example 3 uses an equal amount of hydrolyzed collagen to replace bovine bone collagen peptide.

[0053] A composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis comprises the following components in percentage by mass: 0.0125% taurine, 0.5% vitamin C, 12% type II collagen peptide, 12% type III collagen peptide, 25% red date extract, and the balance hydrolyzed collagen.

[0054] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that Comparative Example 4 uses an equal amount of hydrolyzed collagen to replace the red date extract.

[0055] A composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis comprises the following components in percentage by mass: 0.0125% taurine, 0.5% vitamin C, 12% type II collagen peptide, 12% type III collagen peptide, 25% hydrolyzed collagen, and the remainder bovine bone collagen peptide.

[0056] Comparative Example 5 The difference between Comparative Example 5 and Example 1 is that the amount of each raw material is different, and the others are the same.

[0057] A composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis comprises the following components in percentage by mass: 0.01% taurine, 2% vitamin C, 30% type II collagen peptide, 2% type III collagen peptide, 10% red date extract, and the remainder bovine bone collagen peptide.

[0058] Comparative Example 6 The difference between Comparative Example 6 and Example 1 is that the amount of each raw material is different, and the others are the same.

[0059] A composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis comprises the following components in percentage by mass: 0.5% taurine, 0.05% vitamin C, 2% type II collagen peptide, 30% type III collagen peptide, 40% red date extract, and the remainder bovine bone collagen peptide.

[0060] Test Case 1. Weaned SPF female rats weighing 75-85g were randomly divided into 14 groups, 12 in each group. They were blank control group, model control group, positive control group and test group, and the test groups were the compositions of the embodiments and comparative examples, respectively. Except for the blank control group, other groups were continuously fed with low calcium feed to prepare low bone mass model rats, and each group was fed with the corresponding test product. The positive control group was fed with 150mg / kg calcium carbonate, the test group was fed with 150mg / kg of the corresponding composition, the model control group was given a corresponding volume of distilled water, and the normal control group was fed with normal feed and a corresponding volume of distilled water, all for 10 weeks.

[0061] The low-calcium feed formula is: methionine 0.4%, choline chloride 0.4%, soybean powder 12%, casein 11%, soybean oil 5%, flour 50%, cellulose 1.5%, vitamin 1%, calcium-free mineral salt 2.6%, and starch as the remainder.

[0062] Determination of bone density: One hour after the end of the experiment, the lumbar spine bone density of rats was measured using a dual-energy X-ray absorptiometry.

[0063] Table 1

[0064] Notes: Compared with the blank control group, P<0.05; # Compared with the model control group, P<0.05.

[0065] 2. Refer to the test method for enhancing immunity in the "Functional Evaluation Procedures and Test Methods for Health Foods" to conduct experiments, and conduct ConA-induced mouse spleen lymphocyte transformation experiments: MTT method, to measure the lymphocyte proliferation ability. The stronger the lymphocyte proliferation ability, the stronger the immunity. The dosage is 50g / kg.

[0066] Table 2

[0067] It can be seen from the table that the composition of the present invention can effectively improve bone density and immunity and has broad application prospects.

[0068] Finally, it should be noted that the above embodiments are intended to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis, characterized in that: The invention comprises the following components in percentage by weight: 0.05-0.2% taurine, 0.1%-1% vitamin C, 5%-20% type II collagen peptide, 5%-20% type III collagen peptide and the remainder bovine bone collagen peptide.

2. The composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis according to claim 1, characterized in that: The invention comprises the following components in percentage by mass: 0.01-0.15% taurine, 0.4-0.6% vitamin C, 10-15% type II collagen peptide, 10-15% type III collagen peptide, 24-28% red date extract and the balance bovine bone collagen peptide.

3. The composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis according to claim 1, characterized in that: The preparation method of the bovine collagen peptide is as follows: (1) Selecting cattle shank bones, removing tendons and meat, washing, and crushing to obtain cattle shank bone powder; (2) adding the cattle shank bone powder into an acetic acid solution for soaking and decalcification to obtain decalcified cattle shank bone powder; (3) Add decalcified bovine bone powder, papain, neutral protease, and β-glucanase into water, perform enzymatic hydrolysis at 45-55°C for 2-6 hours, filter, and dry to obtain bovine bone collagen peptide.

4. The composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis according to claim 3, characterized in that: The concentration of the acetic acid solution is 0.05-0.2 mol / L; The mass ratio of the decalcified cattle bone meal, papain, neutral protease, beta-glucanase and water is 1: (0.005-0.02): (0.005-0.02): (0.005-0.02): (4-10).

5. The composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis according to claim 1, characterized in that: The invention also comprises the following components in the following mass percentages: 20-30% red date extract; preferably 24-28% red date extract.

6. The composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis according to claim 2, characterized in that: The preparation method of the red date extract is as follows: (1) crushing red dates to obtain red date powder; (2) adding red date powder, cellulase, alkaline protease and β-glucanase into water, performing ultrasonic enzymolysis at 45-55°C for 2-6 hours to obtain an enzymolysis solution; (3) The pH value of the enzymatic hydrolyzate was adjusted to 6.2-6.8, and the macroporous adsorption resin was applied at a rate of 0.5-1.5 BV / h and an elution rate of 1-2 BV / h. First, 1-2 BV was washed with deionized water, and then 1-3 BV was washed with 40-60 wt% ethanol solution. The ethanol washing liquid was collected and dried to obtain the red date extract.

7. The composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis according to claim 6, characterized in that: The mass ratio of the red date powder, cellulase, alkaline protease, β-glucanase and water is 1: (0.005-0.02): (0.005-0.02): (0.005-0.02): (4-10).

8. The composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis according to claim 6, characterized in that: The power of the ultrasound is 300-600W.

9. The composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis according to claim 6, characterized in that: The macroporous adsorption resin is D101 macroporous adsorption resin.

10. The method for preparing the composition of collagen peptides for enhancing immune stimulation of osteoblast synthesis according to any one of claims 1 to 9, characterized in that: The method comprises the following steps: evenly mixing taurine, vitamin C, type II collagen peptide, type III collagen peptide, red date extract and bovine bone collagen peptide to obtain the product.

Images