Capillary electrochromatography enzyme microreactor based on functionalized graphene and preparation method thereof

A technology of capillary electrochromatography and carboxylated graphene, applied in chemical instruments and methods, enzyme production/bioreactors, biochemical equipment and methods, etc., can solve the problems of complex preparation process, poor separation effect and high production cost , to achieve the effect of simple operation steps, good fixing effect and cost saving
CN107519829AInactive Publication Date: 2017-12-29MINZU UNIVERSITY OF CHINA
5 Cites 9 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
MINZU UNIVERSITY OF CHINA
Publication Date
2017-12-29
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a capillary electrochromatography enzyme microreactor based on functionalized graphene and a preparation method thereof. The preparation method comprises the following steps: (1) pretreating a capillary tube, namely pretreating a capillary tube with alkalis and acids; (2) preparing a graphene substrate based capillary electrochromatography, namely fixing graphene oxide or carboxyl graphene on the inner wall of the capillary tube so as to obtain the graphene substrate based capillary electrochromatography, or preferably fixing the graphene oxide or carboxyl graphene on the inner wall of a whole capillary tube; and (3) preparing an enzyme microreactor, namely introducing a protease solution into one end of the graphene substrate based capillary electrochromatography, performing incubation, and washing with a buffer solution, thereby obtaining the capillary electrochromatography enzyme microreactor based on functionalized graphene. The capillary electrochromatography enzyme microreactor based on functionalized graphene, which is disclosed by the invention, integrates biochemical reactions and separation, and furthermore is high in enzyme immobilization activity, high in separation efficiency for substrates, enzymolysis products and the like, and good in effect.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of biochemical microreactor production, and in particular relates to a functionalized graphene-based capillary electrochromatographic enzyme microreactor and a preparation method. Background technique

[0002] As an important part of the post-gene era, proteomics has become the research frontier and focus in the field of life sciences. In view of the high complexity of protein components and the high disparity in the content of each component, it is particularly critical to develop new protein enzymatic hydrolysis technology and separation and detection technology to realize the rapid separation and efficient identification of various proteins in the system.

[0003] As a new type of micro-biochemical reaction tool, the immobilized enzyme microreactor has the advantages of less sample consumption, high enzymolysis efficiency, reusability, and functional assembly of flowers. It is currently used in protein enzymolysis,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More