Fibre filled capillary pipe electric chromatographic column preparation method

Abstract

The invention discloses a preparation method of a capillary electrochromatography column filled with fiber, comprising the following steps: one end of the fiber is inserted into the capillary, fluid is conveyed into the connection end of the fiber and the capillary for lubrication, the other end of the capillary is connected with a vacuum pump, vacuum is imposed and the fiber is pumped into the capillary. The adopted lubricate fluid is polar solvents, such as methanol, acetone and water and the like; the filled fiber is silica fiber or glass fiber or fiber with bonding functional groups on the surface, the diameter of the fiber is 5 to 450 Mum and the length thereof is 1cm to 50 cm; the capillary forming the capillary electrochromatography column is a fused silica capillary or polyether ketone capillary or metal capillary or capillary with bonding functional groups on the internal surface and the inner diameter of the capillary is 10 to 500 mum. The method of the invention has simple operation, and the needed material is easy to obtain and can fill the fiber into the capillary electrochromatography column conveniently.

Description

technical field [0001] The invention relates to a preparation method of a fiber-filled capillary electrochromatographic column. Background technique [0002] Chromatography, as a conventional analysis method and means of separation, is widely used in various departments of scientific research and various fields of social production. Chromatography is widely used in factory quality inspection systems because it can provide various effective information, such as the composition of the mixture, the content of each component, etc. As a means of separation, chromatography is often used to separate high value-added products that are difficult to separate by other methods. However, as an analysis method, chromatography has the disadvantages of large solvent consumption and environmental pollution. Today, with miniaturization of instruments and chips, traditional chromatography is gradually developing in the direction of microcolumn chromatography, electrochromatography, and electr...

AI Technical Summary

Problems solved by technology

However, the current filling method of fiber-packed columns is backward, and a hard silk thread is often used to tie a section of the fiber, and then guide it into the capillary.

This filling method is primitive and outdated, and it cannot realize the filling of fibers in capillary tubes with small inner diameters (<100 μm). Currently, the inner diameter of commonly used fiber-filled capillary columns is often greater than 250 μm, which greatly affects the improvement of the column efficiency of fiber-filled electrochromatographic columns. restricts its application in practice