Device and method for preparing chromatographic column made of magnetic filling material

Abstract

The invention relates to a device and a method for preparing a chromatographic column made of a magnetic filling material. In the preparation method, magnetic materials or magnetic materials with a shell-core structure are used as a substrate of a chromatographic fixed phase to prepare the chromatographic fixed phase by surface modification, and the chromatographic fixed phase can be used for separation of a capillary electrochromatography, a liquid phase chromatography or a gas phase chromatography; for the filling materials with a bigger grain size, the pneumatic and slurry pole assembling methods and other pole assembling methods are adopted to complete the pole assembly, and an electromagnetic gun device designed in the invention is further used for tamping; and for the fixed phase with the smaller grain size or nanometer grain size, the electric filling method is used for filling, and the electromagnetic gun device is further used for tamping. The invention has the advantages that: the filling device is convenient in use, simple in structure and easy to configure and operate; and the chromatographic column fixed phase of the magnetic filling materials prepared by the method is uniform and dense and has a high chromatographic column efficiency.

Description

【Technical field】 [0001] The invention relates to the technical field of chromatographic columns, in particular to a preparation device and method for a magnetic packing chromatographic column. 【Background technique】 [0002] With the deepening of proteomics research, magnetic materials have been widely used in the research of enriching trace proteins and peptides. Magnetic materials mainly refer to substances that can directly or indirectly generate magnetism from excessive elements such as iron, cobalt, nickel and their alloys. Core-shell magnetic materials mainly refer to composite nano-magnetic materials with a core / shell structure and an insulating shell (such as SiO 2 、Al 2 o 3 , C-SiO 2 etc.) or semiconductor shell (such as ZnO, etc.) composite materials and cores are magnetic materials, because its structure and composition can be designed and tailored on the nanometer scale, so it has many unique optical, electrical, magnetic, catalytic and other physical and ma...

AI Technical Summary

Problems solved by technology

However, for small particle size fillers, due to the electrostatic effect and the increase of surface energy, the aggregation of particles is serious, and it is difficult to install a uniform and compact column bed.

[0005] However, the above methods are not very ideal for the filling of magnetic fillers. For magnetic materials with small particle sizes, especially magnetic nanomaterials, the homogenization method will have the disadvantages of high back pressure and difficult filling.

Using the pneumatic method, that is, dry packing, the magnetic material is prone to agglomeration, and it is difficult to form a uniform and compact column bed

Using the method of electric filling, due to the mutual repulsion between the magnetic material particles, the particles entering the chromatographic column cannot be packed uniformly and tightly

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