Device and method for preparing chromatographic column made of magnetic filling material

A technology of magnetic packing and preparation device, applied in the field of chromatographic columns, can solve the problems of difficult packing, high back pressure, serious particle aggregation, etc., and achieve the effects of easy configuration and operation, uniform stationary phase, and high chromatographic column efficiency

Inactive Publication Date: 2010-06-09
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, for small particle size fillers, due to the electrostatic effect and the increase of surface energy, the aggregation of particles is serious, and it is difficult to install a uniform and compact column bed.
[0005] However, the above methods are not very ideal for the filling of magnetic fillers. For magnetic materials with small particle sizes, especially magnetic nanomaterials, the homogenization method will

Method used

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  • Device and method for preparing chromatographic column made of magnetic filling material
  • Device and method for preparing chromatographic column made of magnetic filling material
  • Device and method for preparing chromatographic column made of magnetic filling material

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Embodiment 1

[0041] 300mg SBF-Fe 3o 4 -SiO 2 -NH 2 For the core-shell magnetic material, add 1mL of methanol, sonicate for 10min, and put it into the capillary column by the method of electrofilling. Put the pre-filled chromatographic column into the enamelled copper electromagnetic coil (1000 turns) → connect to 220V AC, the AC is boosted by the rectifier bridge to become 450V DC to charge the capacitor → capacitor C (select 450V, 990uF) is fully charged, Cut off the 220V power supply → close the power switch K → discharge the capacitor C (select 450V, 990uF) → the current passes through the enamelled copper coil (radius 0.15mm, number of turns 1000 turns) to generate a strong magnetic field in the chromatographic column → pre-filled in the chromatographic column The magnetic or core-shell magnetic filler is accelerated by a strong external magnetic field, and is ejected from one end of the chromatographic column to the other end. ①Repeat the above process of charging and discharging ...

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Abstract

The invention relates to a device and a method for preparing a chromatographic column made of a magnetic filling material. In the preparation method, magnetic materials or magnetic materials with a shell-core structure are used as a substrate of a chromatographic fixed phase to prepare the chromatographic fixed phase by surface modification, and the chromatographic fixed phase can be used for separation of a capillary electrochromatography, a liquid phase chromatography or a gas phase chromatography; for the filling materials with a bigger grain size, the pneumatic and slurry pole assembling methods and other pole assembling methods are adopted to complete the pole assembly, and an electromagnetic gun device designed in the invention is further used for tamping; and for the fixed phase with the smaller grain size or nanometer grain size, the electric filling method is used for filling, and the electromagnetic gun device is further used for tamping. The invention has the advantages that: the filling device is convenient in use, simple in structure and easy to configure and operate; and the chromatographic column fixed phase of the magnetic filling materials prepared by the method is uniform and dense and has a high chromatographic column efficiency.

Description

【Technical field】 [0001] The invention relates to the technical field of chromatographic columns, in particular to a preparation device and method for a magnetic packing chromatographic column. 【Background technique】 [0002] With the deepening of proteomics research, magnetic materials have been widely used in the research of enriching trace proteins and peptides. Magnetic materials mainly refer to substances that can directly or indirectly generate magnetism from excessive elements such as iron, cobalt, nickel and their alloys. Core-shell magnetic materials mainly refer to composite nano-magnetic materials with a core / shell structure and an insulating shell (such as SiO 2 、Al 2 o 3 , C-SiO 2 etc.) or semiconductor shell (such as ZnO, etc.) composite materials and cores are magnetic materials, because its structure and composition can be designed and tailored on the nanometer scale, so it has many unique optical, electrical, magnetic, catalytic and other physical and ma...

Claims

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Application Information

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IPC IPC(8): B01D15/10
Inventor 张维冰马飞雷雯朱亚仙张凌怡
Owner EAST CHINA UNIV OF SCI & TECH
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