Pressurized capillary electrochromatography electrochemistry detecting method

A technology of capillary electrochromatography and detection device, which is used in measurement devices, material electrochemical variables, scientific instruments, etc., to achieve the effects of pressurized capillary electrochromatography separation and electrochemical detection, fast response speed, and selective separation

Inactive Publication Date: 2007-09-19
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently, electrochemical detection has not been applied to p-CEC

Method used

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  • Pressurized capillary electrochromatography electrochemistry detecting method
  • Pressurized capillary electrochromatography electrochemistry detecting method
  • Pressurized capillary electrochromatography electrochemistry detecting method

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Using the electrochemical detection device of the pressurized capillary electrochromatography, the experimental process is carried out according to the experimental process in the specific embodiment, and the detection of 2.0 × 10 -4 mol / L of dienestrol, diethylstilbestrol and hexestrol.

[0020] The experimental conditions are: 30 cm long, 50 μm inner diameter filled with 20 cm, 3 μm particle size ODS capillary, the mobile phase is 31% acetonitrile: 69% buffer, the buffer contains 5mmol / L Tris, 4mmol / L SDS, pH=4.5. The separation voltage is 12kv, the back pressure valve is 1000psi, the pump flow rate is 0.05ml / min, and the working electrode potential is 0.9V. In the figure 1. Dienestrol 2. Diethylstilbestrol 3. Diethylstilbestrol.

[0021] The test results are shown in Figure 2.

Embodiment 2

[0023] Using the electrochemical detection device of the pressurized capillary electrochromatography, the experimental process is carried out according to the experimental process in the specific embodiment, and the detection of 2.0 × 10 -4 mol / L of Sudan Red I, Sudan Red II, Sudan Red III and Sudan Red IV.

[0024] The experimental conditions are: a quartz capillary with a total length of 20 cm and an inner diameter of 50 μm filled with a 1.5 μm particle size ODS filler, the mobile phase is 95% acetonitrile: 5% water, and the water phase contains formic acid (pH=4.3), 5% acetone and 0.002% Tris Ethylamine. The separation voltage is 12kv, the back pressure valve is 500psi, the pump flow rate is 0.1ml / min, and the working electrode potential is 0.95V. In the figure 1. Sudan Red I 2. Sudan Red II 3. Sudan Red III 4. Sudan Red IV.

[0025] The test results are shown in Figure 3.

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Abstract

The present invention provides a pressurized capillary electrochromatography electrochemical detection device comprising a liquid storage tank of mobile phase, a high pressure pump used for carrying the mobile phase and providing liquid to a separation capillary electroosmotic chromatography connected to a detection cell through a microflow controller in which a sample can be input, a high voltage power supply which both ends are applied respectively to a liquid intake terminal of the capillary electrochromatography and a detection cell connected to an electrochemical detector which output terminal signals are connected to a computer through a signal gathering analysis meter. By combining the capillary electrochromatography separation technology and the electrochemical detection technology, the present invention is capable of separating, quickly and efficiently, and detecting, with a high sensitivity, species having electrochemical activity.

Description

technical field [0001] The invention relates to a chemical analysis instrument, in particular to a pressurized capillary electrochromatographic electrochemical detection device. Background technique [0002] Pressurized Capillary Electrochromatography (p-CEC) is a new type of high-efficiency micro-separation technology that gradually emerges on the basis of the continuous development of Capillary Electrophoresis (CE) technology and the increasingly perfect liquid chromatography theory. . Compared with traditional capillary electrophoresis and high-performance liquid chromatography, p-CEC has outstanding advantages such as high efficiency, high separation rate, high selectivity, fast analysis speed, and low reagent consumption, so it has attracted more and more attention from the analytical chemistry community. The detection methods currently used are mainly ultraviolet absorption method, laser-induced fluorescence method, mass spectrometry and nuclear magnetic resonance met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/64G01N27/26G01N30/00
Inventor 谢增鸿刘绍锋吴晓苹林旭聪
Owner FUZHOU UNIV
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