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A kind of polypeptide that specifically targets triple negative breast cancer and its application

A technology of radionuclide and conjugates, which is applied to peptides specifically targeting triple-negative breast cancer and its application fields, can solve problems such as side effects, damage to normal functions of the body, and damage to the body's own immunity.

Active Publication Date: 2021-09-10
TSINGHUA BERKELEY SHENZHEN INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional cancer treatment methods often have serious side effects. While treating cancer, they damage the normal functions of the body and destroy the body's own immunity.

Method used

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  • A kind of polypeptide that specifically targets triple negative breast cancer and its application
  • A kind of polypeptide that specifically targets triple negative breast cancer and its application
  • A kind of polypeptide that specifically targets triple negative breast cancer and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Screening of peptides

[0036] Cells were screened using the Negative- / Positive+ method using the phage peptide library, MCF-7 (negative screen), MDA-MB-231 (positive screen), a total of three rounds. The steps of each round of screening are as follows:

[0037] 1) Add 0.5% BSA to the adherent MCF-7 cells to block for 1 h;

[0038] 2) The phage random dodecapeptide library (Ph.D.-12 Phage Display Peptide Library Kit, New England Bio-LABS, USA) was mixed with 5×10 10 Add the titer of PFU (5 μL) to the MCF-7 cell culture dish and incubate at 37°C for 1 hour (negative sieve);

[0039] 3) Centrifuge at 3000rpm / min for 3min, collect the supernatant (containing phages not bound to non-triple-negative breast cancer cells), transfer to the immunotube where the MDA-MB-231 cells are located, and incubate with shaking at 37°C for 1h (positive screening);

[0040] 4) After the incubation is completed, centrifuge at 3000rpm / min for 3min;

[0041] 5) Discard the supernatant, w...

Embodiment 2

[0060] Fluorescent probe targeting triple-negative breast cancer cells

[0061] A fluorescent probe specifically targeting triple-negative breast cancer cells, comprising amino acid sequence in embodiment 1 as shown in SEQID No.1 polypeptide of number 7 and the fluorophore FAM (carboxyl group) coupled on the peptide chain fluorescein).

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Abstract

The invention discloses a polypeptide specifically targeting triple-negative breast cancer and its application. The polypeptide has the amino acid sequence shown in SEQ ID No.1. The polypeptide has homology with Decorin, and can bind to Decorin receptors on the surface of triple-negative breast cancer cancer cells to play a targeting role. At the same time, the polypeptide has a weak binding ability to other types of breast cancer cells, showing Higher specificity and selectivity can be used for targeted therapy or imaging diagnosis of triple-negative breast cancer.

Description

technical field [0001] The invention relates to the field of medicinal chemistry, in particular to a polypeptide specifically targeting triple-negative breast cancer and its application. Background technique [0002] With the improvement of people's living standards and the extension of average life expectancy, the incidence of cancer worldwide has been increasing year by year, becoming a major factor threatening human health. According to the World Cancer Report, there were 14 million cancer cases worldwide in 2012, and the number will rise to 24 million by 2035. Breast cancer is the second most common cancer in the world and the most common type of cancer among women. In 2012, there were 1.67 million new cases of breast cancer worldwide, accounting for 25% of all new cancer cases that year. According to different molecular types, breast cancer can be divided into: estrogen / progesterone positive type (ER / PR+), HER2 high expression type (HER2+) and triple negative type. F...

Claims

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Application Information

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IPC IPC(8): C07K7/08C12N15/11A61K38/10A61P35/00
CPCA61K38/00A61P35/00C07K7/08
Inventor 马岚胡羽童
Owner TSINGHUA BERKELEY SHENZHEN INST
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