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Carbofuran molecularly imprinted microspheres, preparation and application thereof

A technology of molecularly imprinted microspheres and carbofuran, which is applied in the fields of pesticide residue monitoring and new materials, can solve problems such as the application of molecularly imprinted technology, and achieve the effects of simple preparation process, easy operation and high specific selectivity.

Active Publication Date: 2015-01-21
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the application of molecular imprinting technology in the establishment of analytical methods for carbofuran

Method used

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  • Carbofuran molecularly imprinted microspheres, preparation and application thereof
  • Carbofuran molecularly imprinted microspheres, preparation and application thereof
  • Carbofuran molecularly imprinted microspheres, preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Dissolve 0.5 mmol of the template molecule carbofuran into an acetonitrile-toluene mixture (30 mL, volume ratio: 3:1) to prepare a mixed solution, put the mixed solution in an ice bath and ultrasonically mix for 10-15 minutes, pass nitrogen for 10 minutes to remove oxygen in the system, and place it in a sealed state at 60°C for 24 hours. After the reaction, the polymer solution was centrifuged for 3 min to obtain a white precipitated polymer. The obtained polymer was sequentially subjected to Soxhlet extraction using methanol acetic acid (9:1, v / v) and methanol as the extraction solvent to remove template molecules and interfering substances, and the obtained polymer was dried overnight in a vacuum oven at 50°C The white carbofuran molecularly imprinted microspheres (MIP) were obtained. A control non-imprinted polymer (NIP) was prepared and treated the same except that no template molecule was added.

Embodiment 2

[0034] Dissolve 0.5 mmol of the template molecule carbofuran into acetonitrile (30 mL) containing 2 mmol of functional monomer methacrylic acid, 10 mmol of crosslinking agent divinylbenzene and 0.04 g of initiator azobisisobutyronitrile to prepare a mixed solution, The mixture was placed in an ice bath and ultrasonically mixed for 10-15 minutes, then passed through nitrogen for 10 minutes to remove oxygen in the system, and placed in a sealed state at 60°C for 24 hours. After the reaction, the polymer solution was centrifuged for 3 min to obtain a white precipitated polymer. The obtained polymer was sequentially subjected to Soxhlet extraction using methanol acetic acid (9:1, v / v) and methanol as the extraction solvent to remove template molecules and interfering substances, and the obtained polymer was dried overnight in a vacuum oven at 50°C The white carbofuran molecularly imprinted microspheres were obtained. A control non-imprinted polymer (NIP) was prepared and treated ...

Embodiment 3

[0038] Dissolve 0.5 mmol of template molecule carbofuran into toluene (30 mL) containing 2 mmol of functional monomer methacrylic acid, 10 mmol of crosslinking agent divinylbenzene and 0.04 g of initiator azobisisobutyronitrile to prepare a mixed solution, The mixture was placed in an ice bath and ultrasonically mixed for 10-15 minutes, then passed through nitrogen for 10 minutes to remove oxygen in the system, and placed in a sealed state at 60°C for 24 hours. After the reaction, the polymer solution was centrifuged for 3 min to obtain a white precipitated polymer. The obtained polymer was sequentially subjected to Soxhlet extraction using methanol acetic acid (9:1, v / v) and methanol as the extraction solvent to remove template molecules and interfering substances, and the obtained polymer was dried overnight in a vacuum oven at 50°C The white carbofuran molecularly imprinted microspheres were obtained. Control non-imprinted polymers were prepared and treated identically exc...

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Abstract

The invention discloses a preparation method of carbofuran molecularly imprinted microspheres, comprising the following steps of: dissolving template molecules and a functional monomer into a pore-forming agent solution containing a cross-linking agent and an initiator so as to obtain a reaction mixture, carrying out ultrasonic degassing, sparging nitrogen for deoxygenation, carrying out polymerization in a sealed state, carrying out centrifugation after complete reaction so as to obtain a white powdery polymer, carrying out Soxhlet extraction respectively by the adoption of a methanol acetate mixed liquor and methanol as extraction solvents, drying under vacuum, and removing the solvents so as to obtain the carbofuran molecularly imprinted microspheres. The invention also discloses the carbofuran molecularly imprinted microspheres prepared by the preparation method and an application thereof. The carbofuran molecularly imprinted microspheres obtained in the invention has specific selectivity for carbofuran in agricultural products, can be used to detect residual pesticides on agricultural products rapidly, sensitively, accurately and efficiently, and also can be used as a solid-phase extraction filler.

Description

technical field [0001] The invention relates to the fields of pesticide residue monitoring and new materials, in particular to a molecularly imprinted microsphere for selectively separating carbofuran. Background technique [0002] Carbofuran belongs to carbamate pesticides, is a broad-spectrum insecticide and nematicide, and has contact and stomach poisoning effects. It binds irreversibly to cholinesterase and is therefore highly toxic. It can be absorbed by the roots of plants and transported to various organs of plants, most of which are leaf margins. In recent years, carbofuran has been widely used as the main substitute of organochlorine pesticides, which has brought potential threats to the environment and food safety. Especially in recent years, food safety accidents have occurred frequently. The research on the detection method of Budweiser pesticide residues has also become the focus of research. [0003] At present, the main assay methods are high performance li...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F212/36C08F220/06C08J9/26B01J20/26B01J20/285B01J20/30
Inventor 齐沛沛王新全徐浩章虎王祥云王强李振
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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