Glycine diagnosis/measuring reagent kit and glycine concentration determination method
A technology for concentration determination and glycine, which is applied in the field of medical/food inspection and determination, and can solve problems such as affecting health and nutritional imbalance.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0027] The glycine diagnosis / measurement reagent of this embodiment is a single reagent, including:
[0028] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0029] Stabilizer 500mmol / L
[0030] Coenzyme 3mmol / L
[0031] Glycine dehydrogenase 10000U / L
[0032] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.
[0033] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested glycine sample to the reagent is 1 / 25, the reaction The direction is positive reaction (rising reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.
[0034] After adding the sample and reagent, let them mix and react, and finally put the reactant under the biochemical ana...
Embodiment 2
[0036] The glycine diagnosis / measurement reagent of this embodiment is a double reagent, including:
[0037] Reagent 1
[0038] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0039] Stabilizer 50mmol / L
[0040] Coenzyme 3mmol / L
[0041] Reagent 2
[0042] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0043] Stabilizer 50mmol / L
[0044] Glycine dehydrogenase 10000U / L
[0045] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.
[0046] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested glycine sample to reagent 1 and reagent 2 is 2 / 20 / 5, the reaction direction is positive reaction (rising reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.
[0047] A...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com