The invention discloses a group of KASP primers for detecting
insecticide resistance mutation of
aphis gossypii glover and green peach
aphid and application of the KASP primers, and relates to the technical field of agricultural
biology. The KASP labeled primer group disclosed by the invention comprises a primer combination which is used for detecting resistance mutations of AgoyV62I, AgoyR81T, AgoyK264E, MperR81T, MperV101I, AgoyM918V, AgoyM918LAC, AgoyL1014FCT, MperM918LAT, MperL1014FCT, MperS431F, MperM918TTC, MperM918I, AgoyA302S, AgoyS431F and MperA302G to
imidacloprid,
deltamethrin,
carbofuran,
pirimicarb and
fipronil. And detecting the
drug resistance mutation of the
aphis gossypii glover or green peach
aphid by using one or more primer combinations. The group of KASP labeled primers is good in specificity and high in sensitivity, and can accurately detect the
mutation frequency of the
drug resistance of the
aphis gossypii glover or green peach
aphid to the insecticide.