Biological compound seed coating agent and application

A biological and seed technology, applied in the direction of biocide, application, biocide, etc., to achieve the effect of uniform coating, operator and environment safety, and easy operation

Inactive Publication Date: 2016-04-20
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the biological compound seed coati

Method used

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  • Biological compound seed coating agent and application
  • Biological compound seed coating agent and application
  • Biological compound seed coating agent and application

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0027] Example 1 Acquisition, identification and preservation of Bacillus amyloliquefaciens YN2010-KC

[0028] 1. Obtainment of Bacillus amyloliquefaciens YN2010-KC of the present invention

[0029] 1.1 Material

[0030] Collected from the soil around the rhizosphere of maize at Houshan Farm of Yunnan Agricultural University.

[0031] 1.2 Medium preparation

[0032] Melt LB solid medium and PSA medium (potato sucrose agar medium), pour them into sterilized petri dishes, and cool them to make LB and PSA plates.

[0033] 1.3 separation culture

[0034] Using dilution plate separation and plate streaking separation, a strain obtained by separation and purification was named YN2010-KC.

[0035] 2. Identification of Bacillus amyloliquefaciens YN2010-KC

[0036] 2.1 Morphological identification

[0037] The strain YN2010-KC was cultured on LB medium and cultured at 37°C for 36 hours. Morphological observation and identification were carried out. The bacterial cell was rod-shaped, round-end, periph...

Example Embodiment

[0042] Example 2: Preparation of No. 1 biological composite seed coating agent of the present invention

[0043] Formula 1 of the biological composite seed coating agent of the present invention: in terms of mass fraction, 30% of the fermentation broth of Bacillusamyloliquefaciens YN2010-KCCGMCCNo.5722, and the fermentation broth contains Bacillusamyloliquefaciens YN2010-KCCGMCCNo.5722 The effective number of viable bacteria is 1×10 8 cfu / ml, imidacloprid original drug 1%, tebuconazole original drug 2%, agricultural milk 6022%, xanthan gum 0.5%, the balance is water.

[0044] The preparation of the biological composite seed coating agent No. 1 of the present invention: According to the formula, heat the water to 50°C, stir and add 0.5% of xanthan gum to completely dissolve it into a film-forming solvent, and then add 1% of imidacloprid technical and tebuconazole Mix 2% of the original medicine and 6022% of the agricultural milk evenly, mill in a sand mill for 4 hours to prepare a s...

Example Embodiment

[0049] Example 3 Preparation of No. 2 biological composite seed coating agent of the present invention

[0050] No. 2 formula of the biological composite seed coating agent of the present invention: in terms of mass fraction, 30% of the fermentation broth of Bacillusamyloliquefaciens YN2010-KCCGMCCNo.5722, and Bacillusamyloliquefaciens YN2010-KCCGMCCNo.5722 in the fermentation broth The effective number of viable bacteria is 1×10 8 cfu / ml, carbofuran original drug 3%, thiram original drug 2%, NNO2%, xanthan gum 0.5%, sodium carboxymethyl cellulose 0.5%, the balance is water.

[0051] The preparation of the biological composite seed coating agent No. 2 of the present invention: According to the formula, heat water to 50°C, stir and add 0.5% xanthan gum and 0.5% sodium carboxymethyl cellulose to completely dissolve into a film-forming solvent, and then add Carbofuran original drug 3%, thiram original drug 2% and NNO2% were mixed uniformly, and then milled in a sand mill for 4 hours t...

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Abstract

The invention discloses a biological compound seed coating agent and an application. The biological compound seed coating agent comprises bacillus amyloliquefaciens YN2010-KC CGMCC No.5722 fermentation liquor, insecticide, bactericide, a dispersing agent and a film-forming agent. The biological compound seed coating agent has multiple functions of preventing diseases, preventing insects, enduring drought, promoting growth and increasing the yield, and the problem of pathogenic bacterium insecticide resistance and environmental pollution caused by a traditional chemical seed coating agent can be relieved. The preventing effect of the traditional biological seed coating agent and stability under the adverse conditions can be improved. The biological compound seed coating agent is good in suspension property and dispersing performance, does not precipitate, is even in coating, does not fall off and is safe for operators and environment.

Description

technical field [0001] The invention relates to the technical field of seed treatment, in particular to a biological compound seed coating agent compounded and processed with Bacillus amyloliquefaciens as an active ingredient, plus low-toxicity insecticides, fungicides and related auxiliaries, suitable for Seed treatment of corn, rice, wheat and other gramineous crops. Background technique [0002] Seed coating agents use insecticides, fungicides, herbicides, compound fertilizers, plant growth regulators, trace elements and microorganisms as active ingredients, and film-forming agents, suspending agents, antifreeze agents, preservatives and stabilizers, etc. As an auxiliary component, it is made through special processing technology, and can be directly or diluted and wrapped on the surface of seeds to form a pesticide preparation with a certain strength and permeability protective film. [0003] In the 1930s, the United Kingdom developed seed coating agents for the first t...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N51/00A01N47/22A01N47/26A01N43/653A01P21/00A01P3/00A01P7/04
CPCA01N43/653A01N47/22A01N47/26A01N51/00A01N63/10A01N2300/00
Inventor 何月秋吴毅歆何鹏搏陈丽华张晓梅
Owner YUNNAN AGRICULTURAL UNIVERSITY
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