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Biological compound seed coating agent and application

A biological and seed technology, applied in the direction of biocide, application, biocide, etc., to achieve the effect of uniform coating, operator and environment safety, and easy operation

Inactive Publication Date: 2016-04-20
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the biological compound seed coating agent made from Bacillus amyloliquefaciens and chemical pesticides

Method used

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  • Biological compound seed coating agent and application
  • Biological compound seed coating agent and application
  • Biological compound seed coating agent and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Acquisition, Identification and Preservation of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KC

[0028] 1. Obtaining of Bacillus amyloliquefaciens YN2010-KC of the present invention

[0029] 1.1 Materials

[0030] The soil was collected from the soil around the corn rhizosphere of the Houshan Farm of Yunnan Agricultural University.

[0031] 1.2 Culture medium preparation

[0032] Melt LB solid medium and PSA medium (potato sucrose agar medium), pour them into sterilized petri dishes, cool down, and make LB and PSA plates.

[0033] 1.3 Isolation and culture

[0034] A strain isolated and purified was named YN2010-KC by using dilution plate separation method and plate streak separation.

[0035] 2. Identification of Bacillus amyloliquefaciens YN2010-KC

[0036] 2.1. Morphological identification

[0037]The bacterial strain YN2010-KC was cultured on LB medium, cultured at 37°C for 36 hours, and then observed and identified by morphologica...

Embodiment 2

[0042] Embodiment 2: the preparation of No. 1 biological composite seed coating agent of the present invention

[0043] Formula No. 1 of the biological compound seed coating agent of the present invention: in terms of mass fraction, 30% of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KCCGMCCNo. The effective number of viable bacteria is 1×10 8 cfu / ml, imidacloprid technical 1%, tebuconazole technical 2%, agricultural milk 6022%, xanthan gum 0.5%, and the balance is water.

[0044] The preparation of No. 1 biological compound seed coating agent of the present invention: measure according to the formula, heat the water to 50°C, add 0.5% xanthan gum with stirring, completely dissolve into the film-forming solvent, then add 1% imidacloprid original drug and tebuconazole Mix 2% of the original drug and Nongru 6022% evenly, grind in a sand mill for 4 hours to prepare a suspension, and finally add 30% of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN201...

Embodiment 3

[0049] The preparation of embodiment 3 biological composite seed coating agent No. 2 of the present invention

[0050] Formula No. 2 of the biological compound seed coating agent of the present invention: in terms of mass fraction, 30% of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KCCGMCCNo. The effective number of viable bacteria is 1×10 8 cfu / ml, carbofuran 3%, thiram 2%, NNO 2%, xanthan gum 0.5%, carboxymethyl cellulose sodium 0.5%, and the balance is water.

[0051] The preparation of No. 2 biological compound seed coating agent of the present invention: measure according to the formula, heat the water to 50°C, stir and add 0.5% xanthan gum and 0.5% sodium carboxymethyl cellulose, completely dissolve the film-forming solvent, and then add Carbofuran original drug 3%, thiram original drug 2% and NNO2% were mixed evenly, and ground in a sand mill for 4 hours to prepare a suspension, and finally added Bacillus amyloliquefaciens (Bacillus amyloliquefaciens...

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Abstract

The invention discloses a biological compound seed coating agent and an application. The biological compound seed coating agent comprises bacillus amyloliquefaciens YN2010-KC CGMCC No.5722 fermentation liquor, insecticide, bactericide, a dispersing agent and a film-forming agent. The biological compound seed coating agent has multiple functions of preventing diseases, preventing insects, enduring drought, promoting growth and increasing the yield, and the problem of pathogenic bacterium insecticide resistance and environmental pollution caused by a traditional chemical seed coating agent can be relieved. The preventing effect of the traditional biological seed coating agent and stability under the adverse conditions can be improved. The biological compound seed coating agent is good in suspension property and dispersing performance, does not precipitate, is even in coating, does not fall off and is safe for operators and environment.

Description

technical field [0001] The invention relates to the technical field of seed treatment, in particular to a biological compound seed coating agent compounded and processed with Bacillus amyloliquefaciens as an active ingredient, plus low-toxicity insecticides, fungicides and related auxiliaries, suitable for Seed treatment of corn, rice, wheat and other gramineous crops. Background technique [0002] Seed coating agents use insecticides, fungicides, herbicides, compound fertilizers, plant growth regulators, trace elements and microorganisms as active ingredients, and film-forming agents, suspending agents, antifreeze agents, preservatives and stabilizers, etc. As an auxiliary component, it is made through special processing technology, and can be directly or diluted and wrapped on the surface of seeds to form a pesticide preparation with a certain strength and permeability protective film. [0003] In the 1930s, the United Kingdom developed seed coating agents for the first t...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N51/00A01N47/22A01N47/26A01N43/653A01P21/00A01P3/00A01P7/04
CPCA01N43/653A01N47/22A01N47/26A01N51/00A01N63/10A01N2300/00
Inventor 何月秋吴毅歆何鹏搏陈丽华张晓梅
Owner YUNNAN AGRICULTURAL UNIVERSITY
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