Detection method for disease resistance of cotton verticillium wilt

Abstract

The invention discloses a detection method for disease resistance of cotton verticillium wilt. The detection method comprises the following steps: inoculating a verticillium wilt bacterium into a cotton seedling, detecting the activity of chitinase and / or activity of dextranase in the inoculated cotton seedling; and judging the disease resistance of cotton verticillium wilt according to the activity of chitinase and / or activity of dextranase in the inoculated cotton seedling. According to the detection method, the disease resistance of cotton verticillium wilt is judged by inoculating the verticillium wilt bacterium into the cotton seedling and detecting the activity of chitinase and / or the activity of dextranase in leaves of the inoculated cotton seedling. The method can be used for quickly and accurately acquiring the disease resistance of cotton verticillium wilt in the seedling growing stage without waiting for the cotton seedling to disease. A plant with good disease resistance or tolerance to diseases can be transplanted according to the detection result of the method. Through verification and screening in field morbidity, the anti-disease cotton plants and lines are obtained. The detection method is capable of conveniently obtaining single strains and materials with good disease resistance or tolerance to diseases from the seedling growing stage for direct high yield and quality breeding so as to accelerate the variety breeding process, so that the detection method is suitable for being popularized and applied.

Description

technical field

[0001] The invention belongs to the technical field of cotton disease resistance detection, and in particular relates to a detection method of cotton verticillium wilt disease resistance. Background technique

[0002] Verticillium wilt is a devastating soil-borne vascular disease that can infect a variety of herbaceous crops, woody crops, flowers and woody ornamental plants. Cotton verticillium wilt is one of the important diseases of cotton worldwide. Generally, it hardly appears in the seedling stage. After cotton buds appear in the middle and late stages of growth, a large number of diseases will occur in the field, which will easily lead to the death or wilting of the whole plant. The pathogens of cotton Verticillium wilt are Verticillium dahlia and Verticillium black and white. Because of the variety of pathogenic factors, rapid variation, and the lack of clear genetic rules for disease resistance, breeding disease-resistant varieties is the most importa...

Examples

Embodiment 1

[0067] The detection method of the cotton verticillium wilt disease resistance of the present embodiment comprises the following steps:

[0068] 1) Prepare seeds: select transgenic varieties and their recipient materials: Zhong24 (transfer bivalent (Chi+Glu) gene cotton variety Zhongmian 24), Zhong 24 (Zhongmian 24), susceptible control Ji cotton 11 (SCK), disease-resistant control Yumian 21 (RCK); select grains with plump and uniform grains for future use;

[0069] 2) Sowing and raising seedlings: take the grains selected in step 1), and carry out single-grain sowing in a seedling raising hole with a single hole diameter of 3 cm and a height of 3 cm. The seedling raising hole has 190 holes per plate and two materials are cultivated. After sowing, cover the Sterilized matrix (peat soil) to the thickness of the matrix layer is 1cm, and then cover the soil until the thickness of the soil layer is 2cm; put the seedling raising tray into the artificial pool with plastic cloth on t...

Embodiment 2

[0104] The detection method of the cotton verticillium wilt disease resistance of the present embodiment comprises the following steps:

[0105] 1) Prepare seeds: select varieties or strains with stable seed quality and quality: Yubao 8 × Zhong 24 hybrid F1 (medium-early maturing material, a hybrid of Yubao 8 and Zhong 24), Zhengzamian 13F6 (early-maturing materials, the 6th-generation line selected for Zhengzamian No. 6 hybrid cotton), Jimian 11 (SCK), and Yumian 21 (RCK); select grains with full and uniform grains for future use;

[0106] 2) Sowing seedlings: take the grains selected in step 1), carry out single grain sowing in a seedling tray (6 holes per tray) with a single hole diameter of 3 cm and a height of 3 cm, and then cover the sterilized substrate (peat soil) to The substrate layer is 1cm thick, and then covered with soil until the soil layer is 2cm thick; put the seedling cultivation tray into a germination box with a water depth of 1.5cm to germinate;

[0107] ...

technical field

[0001] The invention belongs to the technical field of cotton disease resistance detection, and in particular relates to a detection method of cotton verticillium wilt disease resistance. Background technique

[0002] Verticillium wilt is a devastating soil-borne vascular disease that can infect a variety of herbaceous crops, woody crops, flowers and woody ornamental plants. Cotton verticillium wilt is one of the important diseases of cotton worldwide. Generally, it hardly appears in the seedling stage. After cotton buds appear in the middle and late stages of growth, a large number of diseases will occur in the field, which will easily lead to the death or wilting of the whole plant. The pathogens of cotton Verticillium wilt are Verticillium dahlia and Verticillium black and white. Because of the variety of pathogenic factors, rapid variation, and the lack of clear genetic rules for disease resistance, breeding disease-resistant varieties is the most importa...

Examples

Embodiment 1

[0067] The detection method of the cotton verticillium wilt disease resistance of the present embodiment comprises the following steps:

[0068] 1) Prepare seeds: select transgenic varieties and their recipient materials: Zhong24 (transfer bivalent (Chi+Glu) gene cotton variety Zhongmian 24), Zhong 24 (Zhongmian 24), susceptible control Ji cotton 11 (SCK), disease-resistant control Yumian 21 (RCK); select grains with plump and uniform grains for future use;

[0069] 2) Sowing and raising seedlings: take the grains selected in step 1), and carry out single-grain sowing in a seedling raising hole with a single hole diameter of 3 cm and a height of 3 cm. The seedling raising hole has 190 holes per plate and two materials are cultivated. After sowing, cover the Sterilized matrix (peat soil) to the thickness of the matrix layer is 1cm, and then cover the soil until the thickness of the soil layer is 2cm; put the seedling raising tray into the artificial pool with plastic cloth on t...

Embodiment 2

[0104] The detection method of the cotton verticillium wilt disease resistance of the present embodiment comprises the following steps:

[0105] 1) Prepare seeds: select varieties or strains with stable seed quality and quality: Yubao 8 × Zhong 24 hybrid F1 (medium-early maturing material, a hybrid of Yubao 8 and Zhong 24), Zhengzamian 13F6 (early-maturing materials, the 6th-generation line selected for Zhengzamian No. 6 hybrid cotton), Jimian 11 (SCK), and Yumian 21 (RCK); select grains with full and uniform grains for future use;

[0106] 2) Sowing seedlings: take the grains selected in step 1), carry out single grain sowing in a seedling tray (6 holes per tray) with a single hole diameter of 3 cm and a height of 3 cm, and then cover the sterilized substrate (peat soil) to The substrate layer is 1cm thick, and then covered with soil until the soil layer is 2cm thick; put the seedling cultivation tray into a germination box with a water depth of 1.5cm to germinate;

[0107] ...

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