Heat-resisting beta-1, 3-1, 4-dextranase and encoding gene thereof

A coding gene and gene technology, applied in the field of β-1, can solve the problems of large differences in glucanase activity and achieve the effects of high expression level, heat resistance characteristics of expression level, and great potential for popularization and application
CN101775385AInactive Publication Date: 2010-07-14CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2010-07-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a beta-1, 3-1, 4-dextranase and an encoding gene thereof. The beta-1, 3-1, 4-dextranase provided by the invention is protein in 1 or 2 or 3 as follows: 1. the protein which is formed from an amino acid sequence shown in 19th-314th bit of a sequence 2 in a sequence table; 2. the protein which is formed from the amino acid sequence shown in the sequence 2 in the sequence table; and 3. the protein which is formed from an amino acid residue sequence of the sequence 2 in the sequence table through the substitution and / or deletion and / or addition of one or more amino acid residues, has the activity of the beta-1, 3-1, 4-dextranase and is derived from 1. A recombined pichia formed by leading the encoding gene of the protein into pichia also belongs to the protecting range of the invention. Experiments prove that the highest enzyme activity of a recombined pichia fermented liquid supernatant can be 55,300U / mL, and crude protein is 9.1g / L. The optimum reaction temperature of the beta-1, 3-1, 4-dextranase of the invention is 70 DEG C, and the optimum pH value is 7.0. The beta-1, 3-1, 4-dextranase has great generalization and application potential in production.
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Description

technical field

[0001] The present invention relates to beta-1,3-1,4-glucanase and its coding gene and high-efficiency expression. Background technique

[0002] Dextran is a class of polymers linked by glucose through different glycosidic bonds, and is the most abundant polysaccharide in nature. β-1,3-1,4-D-glucan is a naturally synthesized polysaccharide, which belongs to the soluble structural non-starch in plant cell walls together with arabinoxylan, pentosan, cellulose, and pectin polysaccharides. Most grains contain β-1,3-1,4-D-glucan, and the highest content in barley is 5-8%, which mainly exists in the cell wall of barley milk. β-1,3-1,4-D-glucan is a linear structure formed by glucose in the β configuration through β-1,3- and β-1,4-mixed bonds, containing about 70% β- 1,4-bond and 30% β-1,3-bond, their molecular weight and configuration vary with barley varieties, natural environment, growth stage and other factors.

[0003] According to the specificity of hydrol...

Claims

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