Cellobiohydrolase mutant and its production method and application

A technology of cellobiose and hydrolase, which is applied to the composition containing enzyme, the mutant of cellobiohydrolase, and the application field of cellulose degradation, can solve the problem of low efficiency of cellulase hydrolysis and accelerate cellobiohydrolase Problems such as hydrolysis efficiency, to achieve the effect of improving hydrolysis effect, improving hydrolysis efficiency and high activity

Active Publication Date: 2021-08-17
NANKAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Another object of the present invention is to provide a cellobiohydrolase mutant to solve the problem of low hydrolysis efficiency of cellulase and accelerate the hydrolysis efficiency of cellobiohydrolase

Method used

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  • Cellobiohydrolase mutant and its production method and application
  • Cellobiohydrolase mutant and its production method and application
  • Cellobiohydrolase mutant and its production method and application

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Embodiment Construction

[0029] The present invention will be further described in detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the description.

[0030] It should be understood that terms such as "having", "comprising" and "including" as used herein do not entail the presence or addition of one or more other elements or combinations thereof.

[0031] The present invention provides a cellobiohydrolase mutant, the enzyme is the following (a) or (b) protein:

[0032] (a) Threonine at position 393 of the amino acid sequence shown in SEQ ID NO: 1 is replaced with other amino acids, the other amino acids being lysine, arginine or glutamic acid,

[0033] (b) A protein derived from (a) in which the amino acid sequence in (a) has been substituted, deleted or added by one or several amino acids and has cellobiohydrolase activity; activity of said cellobiohydrolase mutant Provides a degradation function in cellulose degradation....

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Abstract

The present invention discloses a cellobiohydrolase mutant, whose activity provides the degradation function in cellulose degradation, and the enzyme is the following (a) or (b) protein: (a) as shown in SEQ ID NO: 1 Threonine at position 393 of the amino acid sequence is replaced by other amino acids, the other amino acids are lysine, arginine or glutamic acid, (b) the amino acid sequence in (a) has been substituted, deleted or added or a protein derived from (a) of several amino acids and having cellobiohydrolase activity. The invention also discloses a composition containing one or several enzymes. The invention also discloses the application of cellobiohydrolase mutant or composition in cellulose hydrolysis. The cellobiohydrolase mutant of the present invention has the activity of hydrolyzing cellulose, and a mutant with improved activity is obtained, and the effect of hydrolyzing cellulose is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a cellobiohydrolase mutant, an enzyme-containing composition, a DNA molecule, a recombinant vector, a host cell and a production method of the mutant, as well as its application in cellulose degradation . Background technique [0002] At present, clean and renewable lignocellulosic ethanol fuels are attracting more and more attention. The use of cellulase to degrade lignocellulose to produce bio-based products including ethanol is crucial for sustainable human development. However, the cost of cellulase to degrade lignocellulose is still high, and the cost is mainly limited by the hydrolysis efficiency of cellulase. Therefore, in the process of cellulase degrading lignocellulose, the hydrolysis efficiency of cellulase is a major bottleneck of cellulose conversion. Therefore, scientific researchers have been diligently exploring the research direction of obtaining cellu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12N15/56C12N15/81C12N1/19C12R1/84
CPCC12N9/2437C12N15/815C12Y302/01091
Inventor 蔡文生宗志友邵学广
Owner NANKAI UNIV
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