30 results about "Argininic acid" patented technology

The invention relates to a Rabdosia rubescens (Hemsl.) Hara callus induction medium capable of increasing the content of oridonin and rosmarinic acid. The medium is characterized by comprising the following components: in percent by mass, 3%-5% of an MS medium, 2%-2.5% of 1,5-dehydrated-D-glucitol, 2%-3% of a mixture of KNO3 and (NH4)2SO4, 0.1%-2% of plant polyamine, 2%-5% of oxymatrine, 0.002%-0.004% of ethyl lauroyl arginate hydrochloride, 0.3%-0.5% of agar and the balance deionized water. The medium has the advantage of simultaneously increasing the content of oridonin and rosmarinic acid in the callus of Rabdosia rubescens (Hemsl.) Hara.

Dry syrup preparations comprising loratadine as a hydrophobic medicinal drug are provided. The loratadine dry syrup preparations can be produced using a cellulose material or an argininic acid salt together with sugar.

The invention discloses a polypeptide and a synthesis method thereof. The amino acid sequence of the polypeptide is shown in SEQ ID NO.1; the synthesis method of the polypeptide consists of the following steps: D PMI β Flip along the X axis with Cartesian coordinates to get the mirror counterpart L PMI β ;Will L PMI β Topologically grafted onto the α-helical region of Apamin, thereby getting the grafted L PMI β Apamin; will L PMI β Apamin is converted to D PMI β Apamin; will D PMI β The asparagine at position 2 in the Apamin ring is mutated to arginine, which self-assembles into D-enantiomer small protein supramolecular nanoparticles under the action of hydrophobic interactions and hydrogen bonds; after two-step disulfide bond oxidation, The target peptide is then chemically synthesized by Fmoc D MSN, target peptide D The amino acid sequence of MSN is shown in SEQ ID NO.1; the polypeptide is used to inhibit the growth of cancer cells; the polypeptide of the present invention has a long-acting circulation time in the body, and compared with the traditional small molecule drug of this target, the polypeptide drug has strong specificity , high targeting, and less side effects in the body.

The invention discloses a method for reducing the content of urea and ethyl carbamate in the rice wine brewing process, adding distiller's grain glutenin to the rice wine brewing system for fermentation, and the amount of distiller's grain glutenin added is 0.5g / 100g-8g based on the mass of raw rice / 100g. The present invention effectively extracts gluten by reusing waste rice wine distiller's grains, and adds distiller's grains glutenin at the initial stage of rice wine fermentation to regulate the nitrogen source composition available to yeast in the brewing process, effectively inhibiting the urea cycle and reducing arginine Acid degradation, thereby reducing EC formation.

Provided are a photodegradable hydrogel in which cells can be embedded in the photodegradable gel without causing cytotoxicity when the cells are embedded in the photodegradable gel by allowing the cells to coexist at the time of preparation of the photodegradable gel, and which contains a protein as one of the main components; a culture device using the same; a method for forming tissue; and a method for separating cells. A photodegradable hydrogel is obtained by condensation of an alkyne group contained in a cyclooctyne ring or an azacyclooctyne ring of the following compound A with an azido group of the following compound B. (Compound A) A compound is a photocleavable crosslinker which contains a main chain having a linear type- or a branched type- (of three or more branches) polyethylene glycol structure, a photodegradable nitrobenzyl group disposed at both terminals or a branched terminal of the main chain, and a group having a cyclooctyne ring or an azacyclooctyne ring disposed at a terminal side of the nitrobenzyl group. (Compound B) A compound is an azide-modified protein in which a main chain is a protein and at least some of an amino group present at lysine and arginine side chains of the main chain and an amino group present at a terminal of the main chain are modified with the azido group.

The invention relates to a method for detecting the content of 3-amino-2-piperidone, in particular to a method for detecting the content of 3-amino-2-piperidone in compound amino acid injection. The present invention utilizes high-performance liquid chromatography to detect the content of 3-amino-2-piperidone in the compound amino acid injection, and the chromatographic conditions overcome the weak retention of the C18 chromatographic column, the difficult balance of the amino column and the HILIC chromatographic column, and the ion-pair chromatography It has the advantages of fast column equilibration, excellent specificity, high sensitivity, good accuracy, good reproducibility, low limit of quantitation, etc., and the method is simple to operate and fast in analysis. It uses a strong cation exchange column Compared with the traditional method of manually processing samples with cation exchange resins, it is simpler and faster to directly separate and analyze the diluted samples online. The detection method of the present invention can be used for qualitative or quantitative analysis of 3-amino-2-piperidone in other amino acid products or raw materials such as ornithine hydrochloride and arginine.

The invention belongs to the technical field of fine chemicals, and particularly relates to a preparation method of an environment-friendly high-temperature leveling agent for fabric. Arginine and ethylene oxide are polymerized to prepare leveling agent mother liquor for the first time, and then a final product, the environment-friendly high-temperature leveling agent for the fabric, is prepared from the leveling agent mother liquor, urea and sulfamic acid through a one-pot method. Raw materials are green and environmentally friendly, production conditions are simpler and more convenient, and production is facilitated. The prepared environment-friendly high-temperature leveling agent for the fabric is green and environmentally friendly, and is excellent in transfer dyeing performance (the transfer dyeing rate is as high as 42.6%).

The present invention discloses a cellobiohydrolase mutant, whose activity provides the degradation function in cellulose degradation, and the enzyme is the following (a) or (b) protein: (a) as shown in SEQ ID NO: 1 Threonine at position 393 of the amino acid sequence is replaced by other amino acids, the other amino acids are lysine, arginine or glutamic acid, (b) the amino acid sequence in (a) has been substituted, deleted or added or a protein derived from (a) of several amino acids and having cellobiohydrolase activity. The invention also discloses a composition containing one or several enzymes. The invention also discloses the application of cellobiohydrolase mutant or composition in cellulose hydrolysis. The cellobiohydrolase mutant of the present invention has the activity of hydrolyzing cellulose, and a mutant with improved activity is obtained, and the effect of hydrolyzing cellulose is improved.

Provided are a composition for cell transplant and a method for cell transplant that allow myocytes and / or cardiac progenitor cells to be suitably maintained in myocardial tissue and allow the survival and proliferation of transplanted cells to be improved. This composition for cell transplant comprises cells and an aqueous solution of a protein (A), and is characterized in that: the cells are myocardial cells and / or cardiac progenitor cells; protein (A) has a hydrophobicity of 0.2-1.2; the protein (A) has a polypeptide chain (Y) and / or a polypeptide chain (Y'); the total number of polypeptidechains (Y) and polypeptide chains (Y') in the protein (A) is 1-100; the polypeptide chain (Y) is an amino acid sequence (X) repeated 2-100 times, the amino acid sequence being any one of a VPGVG sequence (1) which is an amino acid sequence represented by SEQ ID NO:1, a GVGVP sequence (2) which is an amino acid sequence represented by SEQ ID NO:2, a GPP sequence, a GAP sequence, and a GAHGPAGPK sequence (3), which is an amino acid sequence represented by SEQ ID NO:3; the polypeptide chain (Y') is the polypeptide chain (Y) where 0.1-5% of the amino acid residues thereof are substituted with lysine residues and / or arginine residues; and the total number of the lysine residues and arginine residues is 1-100.

The invention discloses a recombinant corynebacterium crenatum expressed by Vitreoscilla hemoglobin genes and the applications thereof, pertaining to the technical field of genetic engineering. The recombinant corynebacterium crenatum expressed by Vitreoscilla hemoglobin genes is systemically named as SYPA / pJC1-tac-vgb with the preservation number of CCTCC NO: M 208132. Vgb coming from the Vitreoscilla hemoglobin genes is adopted to construct an expression vector pJC1-tac-vgb of the corynebacterium crenatum; the expression vector pJC1-tac-vgb is electrically shifted into a corynebacterium crenatum SYPA to obtain the recombinant corynebacterium crenatum SYPA / pJC1-tac-vgb; and the Vitreoscilla hemoglobin is successfully expressed, thus improving the oxygen utilization rate of the recombinant corynebacterium crenatum so as to increase the output of L-arginine by fermentation method. The result shows that the acid production capacity of the recombinant bacterium is improved by 13.0 percent than that of an original bacterium, and the bacteria concentration is improved by 10.9 percent.

The invention discloses a catalytic chemistry reaction method which is a technical method employing an alternating electric field to directly catalyze a hydrolysis reaction of ester and amide with high steric hindrance. The technical method is a hydrolysis reaction conducted in an electrolyzer, and a voltage of 20V peak and 1-100 Hz frequency is effected on a hydrolysis system of cedryl acetate, linalyl acetate and N-alpha-benzoyl-DL-arginine-4-nitroaniline. Substrate molecules are polarized in a Helmholtz layer to facilitate fractures of weak bonds, so as to catalyze the hydrolysis reaction without using a catalyst. The method is simply operated, at low costs and without catalyst pollution, and can be used for degradation of organics and pollutants with high steric hindrance.

The present invention addresses the problem of providing a bronchial embolization material that enables tissue repair and replacement. This bronchial embolization material contains a protein (A), and is characterized in that: the protein (A) has a polypeptide chain (Y) and / or a polypeptide chain (Y '); the total number of the polypeptide chains (Y) and the polypeptide chains (Y ') in the protein (A) is 1-100; the polypeptide chain (Y) comprises 2-200 consecutive amino acid sequences (X) of at least one type of amino acid sequence (X) selected from the group consisting of a VPGVG sequence (1), a GVGVP sequence (4), a GPP sequence, a GAP sequence, and a GAHGPAGPK sequence (3), wherein the VPGVG sequence (1) is an amino acid sequence represented by SEQ ID NO: 1; the GVGVP sequence (4) is an amino acid sequence represented by SEQ ID NO: 4; the polypeptide chain (Y ') is a polypeptide chain in which 5% or less of the amino acids in the polypeptide chain (Y) are substituted with lysine and / or arginine, and the total number of lysine and arginine is 1-100; the protein (A) satisfies the following relational expression (1); the density of the bronchial embolism material is 90-450 mg / cm < 3 >; the bronchial embolization material satisfies the following relational expression (2). 0.50 < = [the total number of amino acids constituting the amino acid sequence (X) included in the protein (A) and amino acids constituting the amino acid sequence (X ') included in the protein (A)] / [the total number of amino acids constituting the protein (A)] < = 0.80 (1). The amino acid sequence (X ') is an amino acid sequence in which 60% or less of the amino acids in the amino acid sequence (X) are substituted with lysine and / or arginine. ] 0.01 < = Q / P (2) [In relational expression (2), P is the weight of a dried product (DP) obtained by drying the bronchial embolization material at 1 atmospheric pressure 100 DEG C for 3 hours and then drying at 1 atmospheric pressure 40 DEG C for 15 hours; q is the weight of a substance (DQ) obtained by immersing the dried product (DP) in water at 1,000 times the weight of P at 25 DEG C for 72 hours, then taking out the dried product (DP), drying the dried product (DP) at 100 DEG C for 3 hours, and then drying the dried product (DP) at 40 DEG C for 15 hours.

The invention discloses a 19 The preparation method of the magnetic resonance imaging imaging contrast agent of F-pointan microemulsion, the steps are: (1) the preparation of nanoemulsion: by phospholipid, Pluronic F-68, 19 F‑Point, 19 F-porphyrin and 1,1,1-tris(perfluoro-tert-butoxymethyl)ethane obtain nanoemulsion through ultrasonic emulsification; (2) 19 Preparation of F-Xuefan: Compound obtained by reacting with F-polyethylene glycol 19 F‑Xue Fan. (3) 19 Preparation of F-porphyrin: Reaction of porphyrin with F-polyethylene glycol to obtain compound 19 F‑Porphyrin. (4) Preparation of targeting molecules: Cholesterol-polyethylene glycol 2000-maleimide reacts with arginine-glycine-aspartic acid to obtain targeting molecules. (5) Preparation of targeted nanoemulsion: adding cholesterol-polyethylene glycol 2000-arginine-glycine-aspartic acid into the nanoemulsion, and shaking to obtain the targeted nanoemulsion. The method is simple and efficient, and the contrast agent has high sensitivity and good biocompatibility, can target lung cancer tumors for MR imaging and optical imaging, and is applied to early diagnosis and treatment of lung cancer.