Rabdosia rubescens (Hemsl.) Hara callus induction medium capable of increasing content of oridonin and rosmarinic acid

A technology of oridonin and rosmarinic acid, which is applied in botany equipment and methods, horticulture, gardening tools/equipment, etc., can solve the undiscussed problems such as the correlation between the cultivation process and the accumulation of metabolites, and achieve shortening induction The effect of generating cycle, improving yield and reducing loss

Active Publication Date: 2020-05-12
PROYA COSMETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, in "Study on Suspension Culture Technology of Rubescens japonicus Cells", through the research of various key factors affecting the suspension culture system of Rubescens japonicus cells, the suspension culture system of Rubescens japonicus cells was established, but the culture process and metabolites were not discussed. Correlation between accumulation; "Effects of Nitrogen and Carbon Sources on Callus Growth and Accumulation of Rosmarinic Acid in Rubescens japonicus", by changing the ratio of nitrogen and carbon sources in the medium to increase the growth of rosemary acid production, but the study did not effectively solve the problem of browning, which will seriously affect the survival rate of callus

Method used

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  • Rabdosia rubescens (Hemsl.) Hara callus induction medium capable of increasing content of oridonin and rosmarinic acid
  • Rabdosia rubescens (Hemsl.) Hara callus induction medium capable of increasing content of oridonin and rosmarinic acid
  • Rabdosia rubescens (Hemsl.) Hara callus induction medium capable of increasing content of oridonin and rosmarinic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A callus induction medium for Rubescens chinensis, the specific preparation method is as follows: including 3% MS medium (see Table 1 for composition), 2.5% 1,5-anhydro-D-glucitol, 2.5% KNO 3 and (NH 4 ) 2 SO 4 Mixture, 0.1% plant polyamine, 2% matrine, 0.002% ethyl lauroyl arginine hydrochloride, 0.4% agar, the balance deionized water. where KNO 3 and (NH 4 ) 2 SO 4 The molar ratio is 1.8:1.

Embodiment 2

[0033] A kind of culture medium for callus induction of Rubescens japonicus, the specific preparation method is as follows: including 4% MS medium (see Table 1 for composition), 2% 1,5-anhydro-D-glucitol, 3% KNO 3 and (NH 4 ) 2 SO 4 Mixture, 2% plant polyamines, 5% matrine, 0.004% ethyl lauroyl arginate hydrochloride, 0.35% agar, the balance deionized water. where KNO 3 and (NH 4 ) 2 SO 4 The molar ratio is 3:1.

Embodiment 3

[0035] A callus induction medium for Rubescens chinensis, the specific preparation method is as follows: including 5% MS medium (see Table 1 for composition), 2.5% 1,5-anhydro-D-glucitol, 2% KNO 3 and (NH 4 ) 2 SO 4 Mixture, 0.6% plant polyamines, 3% matrine, 0.003% ethyl lauroyl arginine hydrochloride, 0.35% agar, the balance deionized water. where KNO 3 and (NH 4 ) 2 SO 4 The molar ratio is 2:1.

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Abstract

The invention relates to a Rabdosia rubescens (Hemsl.) Hara callus induction medium capable of increasing the content of oridonin and rosmarinic acid. The medium is characterized by comprising the following components: in percent by mass, 3%-5% of an MS medium, 2%-2.5% of 1,5-dehydrated-D-glucitol, 2%-3% of a mixture of KNO3 and (NH4)2SO4, 0.1%-2% of plant polyamine, 2%-5% of oxymatrine, 0.002%-0.004% of ethyl lauroyl arginate hydrochloride, 0.3%-0.5% of agar and the balance deionized water. The medium has the advantage of simultaneously increasing the content of oridonin and rosmarinic acid in the callus of Rabdosia rubescens (Hemsl.) Hara.

Description

technical field [0001] The invention belongs to the technical field of plant cell culture, and in particular relates to a callus culture medium for Rubescensine A and rosmarinic acid, which can increase the content of oridonin A and rosmarinic acid. Background technique [0002] Donglingcao, also known as Binglingcao and Yanmingcao, is a perennial herbaceous plant belonging to the family Lamiaceae. It is widely grown in central areas such as Henan, Shanxi, and Shaanxi. It is suitable for the growth environment of subtropical and temperate monsoon climates. The aboveground parts of the plant Rich in oridonin and rosmarinic acid, both of which have antioxidant, anti-inflammatory and antibacterial properties. Rubescensine A is a natural organic compound of kaurane-type tetracyclic diterpenoids, which is currently mainly used in clinical anti-tumor drugs with obvious curative effects; rosmarinic acid is a polyphenolic compound, which is widely used in the field of cosmetics and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 崔丽涛黄洁芳陈强李芡芡徐晓明王丽娜毕永贤蒋丽刚
Owner PROYA COSMETICS
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