Preparation method and application of feed additive N-carbamylglutamic acid

A technology of carbamoylglutamic acid and feed additive, which is applied in the field of preparation of feed additive N-carbamylglutamic acid, can solve the problem of insufficient synthesis of N-acetylglutamic acid, high price of arginine, and improvement of feed Cost and other issues, to achieve the effect of low cost, easy access to raw materials, and increased daily weight gain

Inactive Publication Date: 2010-06-30
INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its absorption is antagonistic to lysine, and side effects such as diarrhea occur when excessive intake of exogenous arginine, and the price of arginine is expensive, and the price per ton is as high as more than 100,000 yuan. The cost of feed has been increased, so it is of great significance to find another way to increase the supply of endogenous arginine in pigs
[0003] Some data show that carbamoyl phosphate is necessary for the synthesis of citrulline from ornithine, while N-acetylglutamic acid is necessary for the synthesis of N-acetylglutamate synthase (NAGS) and 5-carboxy-pyrroline Metabolic allosteric activators of the enzyme (pyrroline-5-carboxylate synthase, P5CS) (Susumuk et al., Effects of in vitro addition of disulfide or thiol compounds on the activity of the arginine activator N-acetylglutamate synthase, Molecular Catalysis Substance B; Enzyme, 2000, 10; 191-197), but in the small intestinal mucosa and liver mitochondria of suckling piglets, the synthesis of N-acetylglutamic acid is insufficient due to the deficiency of acetylglutamate synthetase (Uchiama.C et al., Subcellular localization and characteristics of N-acetylglutamate synthetase in mouse small intestine mucosa, Biochemistry, 1981, 89; 1777-1786), therefore, N-acetylglutamate may play a role in regulating cucurbit synthesis in the small intestine of suckling piglets. amino acid and arginine play an important role in the process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A preparation method of feed additive N-carbamylglutamic acid, the steps are:

[0024] a. Take 20L of methanol, 0.30kg of glutamic acid, mix with 2L of sulfuric acid, react at room temperature (the same as below 20-25°C) for 4 hours, and adjust the pH value to 8.5 with triethylamine to obtain methyl L-γ-glutamate;

[0025] b. Add the L-γ-glutamic acid methyl ester obtained in step a dropwise into the mixed solution of 2L methanol and 2L carbon disulfide, while adding dropwise, pass through ammonia gas to saturation, and place it in a sealed place at 32°C for 42h, and then place it in a closed place at 1500Pa Gamma-methyl ester-L-glutamic acid-N-amino acid diamine salt concentrate is obtained after concentration and removal of ammonia under pressure;

[0026] c. heating the concentrated solution of γ-methyl ester-L-glutamic acid-N-amino acid diamine salt obtained in step b to 50° C., adding acetic acid until the pH value is 6.5, and removing carbon disulfide under reduce...

Embodiment 2

[0029] A preparation method of feed additive N-carbamylglutamic acid, the steps are:

[0030] a. Take 15 L of methanol, 0.22 kg of glutamic acid, add 1.5 L of sulfuric acid, react at room temperature for 3 hours, and adjust the pH value to 8.0 with triethylamine to obtain methyl L-γ-glutamate;

[0031] b. Add the L-γ-glutamic acid methyl ester obtained in step a dropwise into the mixed solution of 1.5L methanol and 1.5L carbon disulfide, while adding dropwise, feed ammonia gas to saturation, and place it in a sealed place at 30°C for 40h, After concentration and removal of ammonia under the pressure of 2000Pa, the concentrated solution of γ-methyl ester-L-glutamic acid-N-amino acid diamine salt is obtained;

[0032] c. heating the γ-methyl ester-L-glutamic acid-N-amino acid diamine salt concentrated solution obtained in step b to 45° C., adding acetic acid until the pH value is 6.8, and removing carbon disulfide under reduced pressure under a pressure of 3000 Pa. When a large...

Embodiment 3

[0035] A preparation method of feed additive N-carbamylglutamic acid, the steps are:

[0036] a. Mix 10 L of methanol, 0.15 kg of L-glutamic acid, and 1 L of sulfuric acid, react at room temperature for 2 hours, and adjust the pH value to 7.5 with triethylamine to obtain a solution of methyl L-γ-glutamate;

[0037] b. Add the L-γ-glutamic acid methyl ester solution obtained in step a dropwise into the mixed solution of 1L methanol and 1L carbon disulfide, then pass ammonia gas to saturation, seal it at 29°C, and place it for 39h. Gamma-methyl ester-L-glutamic acid-N-amino acid salt concentrate is obtained after concentration and removal of ammonia under the pressure of 2500Pa;

[0038] c. heating the γ-methyl ester-L-glutamic acid-N-amino acid diamine salt concentrated solution obtained in step b to 42° C., adding acetic acid until the pH value is 7.0, and removing carbon disulfide under 4000 Pa pressure conditions to obtain N- Crude carbamoylglutamic acid;

[0039] d. The c...

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Abstract

The invention discloses a preparation method and application of feed additive N-carbamylglutamic acid. The steps of the preparation method are that: a. L-glutamic acid, methanol and sulfuric acid are mixed and react with each other, and triethylamine is used to regulate the pH value of the mixed solution to obtain L-alpha-methyl glutamate; the obtained L-alpha-methyl glutamate is added into the mixed solution of methanol and carbon disulfide, ammonia is fed in till the mixed solution is saturated, the obtained solution is placed under closed condition, and alpha-methyl ester-L-glutamic acid-N-amino-acid salt concentrated solution is obtained after concentration and ammonia removal; the concentrated solution is heated, acetic acid is added, the pressure is reduced to remove carbon disulfide to obtain crude N-carbamylglutamic acid, the crude N-carbamylglutamic acid is decolorized by active carbon and is recrystallized to obtain the finished product; and b. the N-carbamylglutamic acid is crushed and passes through a sieve, the obtained product is mixed with premix, and the obtained product is put and agitated in an agitator and is prepared into particles. The feed additive N-carbamylglutamic acid can promote the synthesis of endogenous arginine in bodies of piglets and sows. The method is simple, the operation is simple and convenient, the raw materials can be obtained easily and the cost is low. The produced feed can promote the synthesis of piglet proteins, relieve weanling stress of the piglets and improve the productivity of the sows.

Description

technical field [0001] The invention relates to the technical field of pig feed additives, more specifically to a preparation method of feed additive N-carbamylglutamic acid, and also to the use of the feed additive in promoting the growth performance of piglets and the reproductive performance of sows. It is suitable for In animal husbandry production, it can increase the growth rate of piglets, relieve weaning stress, increase the number of piglets born alive in sow litters, and increase the birth weight of piglets. Background technique [0002] Arginine is an essential amino acid during fetal and lactation periods in mammals (Wu et al., Arginine requirements of piglets, Journal of Nutrition, 2004). The amount of arginine in breast milk and the endogenous synthesis of suckling piglets can not meet the needs of their growth. The lack of arginine is a major factor limiting the growth of suckling piglets. The production of suckling piglets is very important to the whole pig ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C275/16C07C273/18A23K1/16A23K1/18
Inventor 印遇龙黄瑞林谭碧娥
Owner INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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