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A kind of polypeptide and its synthesis method

A synthesis method, amino acid technology, applied in the preparation method of peptides, peptide/protein components, chemical instruments and methods, etc., can solve the problems of limiting clinical application potential, test failure, etc., achieve excellent in vivo stability, small in vivo side effects, The effect of enhancing performance

Active Publication Date: 2021-09-21
陕西未来多肽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, no MDM2 antagonists targeting this target have been approved for marketing, and some inhibitors have limited their clinical application potential due to limited efficacy in preclinical studies. Potential drugs at the point of failure in clinical trials due to toxic effects

Method used

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  • A kind of polypeptide and its synthesis method
  • A kind of polypeptide and its synthesis method
  • A kind of polypeptide and its synthesis method

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Experimental program
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Effect test

Embodiment 1

[0043] Experimental Materials and Instruments

[0044] Table 1 Experimental reagents and manufacturers

[0045] Reagent manufacturer FBS Gibco Fluorescein isothiocyanate (FITC) Sigma-Aldrich MTT Dojindo EDTA Sigma-Aldrich Sodium azide Sigma-Aldrich HEPES Sigma-Aldrich Tween20 Sigma-Aldrich β-mercaptoethanol Sigma-Aldrich Sodium Dodecyl Sulfate (SDS) Sigma-Aldrich 30% acrylamide Biorad Glycine Sigma-Aldrich Trizma base Sigma-Aldrich TEMED Sigma-Aldrich Sodium chloride Sigma-Aldrich Methanol Sigma-Aldrich Ammonium persulfate Sigma-Aldrich potassium chloride Sigma-Aldrich Na 2 HPO 4

Sigma-Aldrich K H 2 PO 4

Sigma-Aldrich Horseradish-labeled goat anti-mouse secondary antibody Calbiochem ECL substrate Biorad RIPA lysate Sigma-Aldrich BCA Protein Quantification Kit Thermo Fisher Anti-β-catenin anti...

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Abstract

The invention discloses a polypeptide and a synthesis method thereof. The amino acid sequence of the polypeptide is shown in SEQ ID NO.1; the synthesis method of the polypeptide consists of the following steps: D PMI β Flip along the X axis with Cartesian coordinates to get the mirror counterpart L PMI β ;Will L PMI β Topologically grafted onto the α-helical region of Apamin, thereby getting the grafted L PMI β Apamin; will L PMI β Apamin is converted to D PMI β Apamin; will D PMI β The asparagine at position 2 in the Apamin ring is mutated to arginine, which self-assembles into D-enantiomer small protein supramolecular nanoparticles under the action of hydrophobic interactions and hydrogen bonds; after two-step disulfide bond oxidation, The target peptide is then chemically synthesized by Fmoc D MSN, target peptide D The amino acid sequence of MSN is shown in SEQ ID NO.1; the polypeptide is used to inhibit the growth of cancer cells; the polypeptide of the present invention has a long-acting circulation time in the body, and compared with the traditional small molecule drug of this target, the polypeptide drug has strong specificity , high targeting, and less side effects in the body.

Description

technical field [0001] The invention belongs to the field of biomedicine and anticancer, and in particular relates to a polypeptide and a synthesis method thereof. Background technique [0002] Malignant tumors have become one of the major diseases that are increasingly common and seriously threaten human life and quality of life. Primary hepatic carcinoma is a malignant tumor caused by abnormal proliferation of liver cells. It is highly malignant and progresses rapidly, with poor prognosis and often endangers the lives of patients. Its death rate ranks fifth among malignant tumors in my country. Colorectal cancer and liver cancer are both digestive tract tumors, and are among the top 10 malignant tumors in my country. It can be seen that digestive tract tumors closely related to diet have seriously threatened the health of Chinese residents. At present, the clinical treatment methods for liver cancer and colorectal cancer at home and abroad are mainly surgical resection, r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08C07K1/06C07K1/04A61K38/10A61P35/00
CPCA61K38/00A61P35/00C07K7/08
Inventor 何旺骁闫瑾高汝青
Owner 陕西未来多肽生物科技有限公司
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