Application of arginine in preparation of preservative for improving generation capacity and biocontrol effect of Metschnikowia citriensis pulcherriminic acid

A technology of generating capacity and arginine, applied in the preservation and application of food ingredients as antimicrobials, preservation of fruits and vegetables, etc., can solve the problems of large difference in effect, high production cost, short shelf life, etc., to solve the problem of agricultural residues and environmental pollution problems, good application potential, effect of enhancing efficacy

Active Publication Date: 2021-04-02
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with the traditional general-purpose chemical fungicides, there is still a certain gap in the effect of these commercial biocontrol agents and the antagonistic yeast with potential commercial value reported by research.
The main reasons are: the effect of antagonistic yeasts in the actual application process is quite different from that in the laboratory research; the stability is not good due to the influence of factors such as the concentration of pathogenic bacteria, the physiological state of the fruit, and the storage environment; the shelf life is short, and it is difficult to develop long-term effective formulations ; high production costs, etc.
These problems lead to slow progress in the large-scale commercial application of antagonistic yeast

Method used

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  • Application of arginine in preparation of preservative for improving generation capacity and biocontrol effect of Metschnikowia citriensis pulcherriminic acid
  • Application of arginine in preparation of preservative for improving generation capacity and biocontrol effect of Metschnikowia citriensis pulcherriminic acid
  • Application of arginine in preparation of preservative for improving generation capacity and biocontrol effect of Metschnikowia citriensis pulcherriminic acid

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Embodiment Construction

[0024] In order to make the object, technical solution and beneficial effect of the present invention clearer, preferred embodiments of the present invention will be described in detail below.

[0025] 1. Experimental method

[0026] 1. Effects of different amino acids on the production of M.citriensis pucherin

[0027] With different concentrations of amino acids (0, 1, 5, 10, 100mmol·L -1 ) PDA plate to test the effect of amino acids on the production of pucherin by M.citriensis. Fixed content of 35mg·L -1 FeCl 3 The volume of PDA medium on each plate is 10 mL. After the medium is fully solidified, take 20 μL of 1×10 8 cells mL -1 The M.citriensis bacterial suspension was evenly spread on the central axis of the PDA plate, and the spreading range was 60mm×2.6mm. After the PDA plate was cultured at 28°C for 3 days, the width of the pucemin pigment circle was measured. A total of 20 amino acids were tested: alanine (Ala), valine (Val), leucine (Leu), isoleucine (Ile), ...

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Abstract

The invention discloses an application of arginine in preparation of a preservative for improving the pulcherriminic acid production capacity of Metschnikowia citriensis, and a method for improving the pulcherriminic acid production capacity of the Metschnikowia citriensis by using the arginine. The invention further provides application of the arginine in preparation of a preservative for improving the biological prevention and treatment effect of the Metschnikowia citriensis on postharvest diseases of fruits, and a method for improving the biological prevention and treatment effect of the Metschnikowia citriensis on the postharvest diseases of the fruits by utilizing the arginine. The effect is clear and remarkable, the problems of agricultural residues and environmental pollution causedby the use of pesticide can be effectively solved, good application potential is realized in the aspect of biological prevention and treatment of the postharvest diseases of the fruits, and meanwhile, an excellent method is provided for researching and developing microbial pesticides and enhancing the efficacy of the microbial pesticides.

Description

technical field [0001] The invention belongs to the technical field of post-harvest disease biological control of fruits, and relates to the application of arginine in the preparation of a preservative for improving the ability of Metschnikowia citriensis to produce clementine acid and its biocontrol efficacy. Background technique [0002] Since Wilson and Pusey reviewed the application potential of using antagonistic bacteria to carry out postharvest biological control of postharvest fruits in 1985, the research reports on antagonistic yeasts have shown exponential growth, and the research on using antagonistic yeasts to control postharvest fruit diseases has also made great progress. progress, some antagonistic yeasts with good comprehensive properties have also achieved commercial applications, such as Candida oleophila (Aspire TM , Ecogen, Langhorne, PA, US), Cryptococcus albidus (YieldPlus TM , Lallemand, Montreal, Canada), Candida sake (Candifruit TM , IRTA, Lleida, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/155C12N1/38C12R1/645
CPCA23B7/155C12N1/38A23V2002/00A23V2200/10Y02A40/90
Inventor 曾凯芳王淑培易兰花邓丽莉姚世响阮长晴
Owner SOUTHWEST UNIVERSITY
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